A5089025407- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Cancer Research and Treatments
- Telomeres, Telomerase, and Senescence
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Cell Image Analysis Techniques
- RNA and protein synthesis mechanisms
- Circular RNAs in diseases
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Mathematical Biology Tumor Growth
- Virus-based gene therapy research
- Immune cells in cancer
- Esophageal Cancer Research and Treatment
- Chemical Reactions and Isotopes
- RNA Research and Splicing
- Fibroblast Growth Factor Research
- Cancer, Hypoxia, and Metabolism
- Polyomavirus and related diseases
Hiroshima University
2018-2024
Higashihiroshima Medical Center
2020
National Cancer Research Institute
2018
Tokyo Institute of Technology
2006-2008
Research Institute for Bioscience and Biotechnology
2006-2007
Cancer-associated fibroblasts (CAFs), one of the major components a tumour microenvironment, comprise heterogeneous populations involved in progression. However, it remains obscure how CAF heterogeneity is governed by cancer cells. Here, we show that extracellular vesicles (EVs) induce series chemokines activated and contribute to formation heterogeneity. In xenograft model diffuse-type gastric cancer, showed two distinct fibroblast subpopulations with alpha-smooth muscle actin (α-SMA)...
Abstract Background Acquisition of drug-resistance in cancer has led to treatment failure, however, their mechanisms have not been clarified yet. Recent observations indicated that aberrant expressed microRNA (miRNA) caused by chromosomal alterations play a critical role the initiation and progression cancer. Here, we performed an integrated genomic analysis combined with array-based comparative hybridization, miRNA, gene expression microarray elucidate mechanism drug-resistance. Results...
Drug resistance is a major obstacle in the treatment of breast cancer. Surviving cells lead to tumor recurrence and metastasis, which remains main cause cancer-related mortality. Breast cancer also highly heterogeneous, hinders identification individual with capacity survive anticancer treatment. To address this, we performed extensive single-cell gene-expression profiling luminal-type cell line MCF7 its derivatives, including docetaxel-resistant cells. Upregulation epithelial-to-mesenchymal...
Emerging evidence indicates that small RNAs, including microRNAs (miRNAs) and their isoforms (isomiRs), transfer RNA fragments (tRFs), are differently expressed in breast cancer (BC) can be detected blood circulation. Circulating RNAs extracellular vesicles (EVs) have emerged as ideal markers RNA-based applications for detection. In this study, we first undertook sequencing to assess the expression of circulating serum BC patients cancer-free individuals (controls). Expression 3 namely...
Alteration of RNA splicing is a hallmark cellular senescence, which associated with age-related disease and cancer development. However, the roles factors in senescence are not fully understood. In this study, we identified factor PRPF19 as critical regulator normal human diploid fibroblasts. was downregulated during replicative knockdown prematurely induced senescence-like cell cycle arrest through p53-p21 pathway. RNA-sequencing analysis revealed that caused switch MDM4 isoform from stable...
A number of studies report that epithelial to mesenchymal transition (EMT) supports the generation and maintenance cancer stem cells (CSCs), which show tumor seeding ability drug resistance; however, molecular mechanisms underlying induction EMT-associated malignancy remain unclear. The present study reports oral switch from expressing CD44 variant form (CD44v) standard (CD44s) during acquisition cisplatin-resistance, resulted in EMT induction. CD44s induced an phenotype cisplatin resistant...
Drug resistance is a major problem for breast cancer patients. Docetaxel an anti-mitotic agent that serves as first line of treatment in metastatic cancer, however it susceptible to cellular drug resistance. Drug-resistant cells are able spread during treatment, leading failure and eventually metastasis, which remains the main cause cancer-associated death. In previous studies, we used single-cell technologies identified set genes exhibit increased expression drug-resistant cells, they...
Abstract Although functional abnormalities in microRNAs, a class of small non-coding RNAs, have been implicated the pathogenesis various cancer types, nature their dysfunction as an integrated cellular system remains poorly understood. Here, we demonstrate that oncofetal protein IGF2BP3 selectively regulates production 3’-isoforms (3’-isomiRs), including miR-21-5p and members Let-7 family. This regulation induces significant changes seed occupancy structural composition, thereby establishing...
The SV40 capsid is composed primarily of 72 pentamers the VP1 major protein. Although also contains minor protein VP2 and its amino-terminally truncated form VP3, their roles in assembly remain unknown. An vitro system was used to investigate role recombinant pentamers. Under physiological salt pH conditions, alone remained dissociated, at 5.0, it assembled into tubular structures. A stoichiometric amount allowed spherical particles a range 7.0 4.0. Electron microscopy observation, sucrose...
Ribophorin II ( RPN2 ), part of the N ‐oligosaccharyltransferase complex, is highly expressed in breast cancer stem cells and associated with tumor metastasis through interaction mutant p53. The clinicopathological implication expression undetermined. We examined immunohistochemically levels p53 primary tissues surgically resected from 218 patients. correlations intrinsic subtype defined by hormone receptors HRs ) HER2 , parameters, expression, patients’ clinical outcomes were examined. was...
Sarcoma is a rare cancer with several subtypes; therefore, our understanding of the pathogenesis sarcoma limited, and designing effective treatments difficult. Circulating microRNAs (miRNAs), including exosomal miRNAs, have attracted attention as biomarkers in cancer. However, roles miRNAs exosomes remain unclear. The present analysis tissue serum miRNA expression osteosarcoma, Ewing's dedifferentiated liposarcoma (DDLPS) identified miR‑1246, ‑4532, ‑4454, ‑619‑5p ‑6126 for DDLPS. These were...
The simian virus 40 (SV40) particle is mainly composed of the major capsid protein termed VP1. VP1 self‐assembles into virus‐like particles (VLPs) approximately nm in diameter when over‐expressed bacteria or insect cells, but purified does not form such a structure under physiological conditions, and thus, mechanism assembly well understood. Using highly assembly/disassembly system vitro , here we provide evidence that DNA factor contributes to 40‐nm spherical particles. At pH 5, for...
Background: The utility of telomere G-tail length to predict coronary artery disease (CAD) remains controversial. CAD results from narrowing due cholesterol and lipid accumulation, augmented by inflammatory cells other factors. This study explored the significance in suspected patients. Methods Results: In all, 95 patients with or ≥1 cardiac risk factor underwent computed tomography angiography (CCTA). We measured leukocyte using a hybrid protection method, diagnosed presence CCTA....
Cisplatin (cis-diamminedichloroplatinum II [CDDP] ) is a well-known chemotherapeutic drug that has been used for the treatment of various types human cancers, including head and neck cancer. exerts anticancer effects by causing DNA damage, replication defects, transcriptional inhibition, cell cycle arrest, induction apoptosis. However, resistance one most serious problems with cancer chemotherapy, it causes expected therapeutic to not always be achieved. Here, we analyzed global microRNA...
The nature of microRNA (miRNA) dysfunction in carcinogenesis remains controversial because the complex connection between miRNA structural diversity and biological processes. Here, we found that oncofetal IGF2BP3 regulates selective production a subset 3′-isoforms (3′-isomiRs), including miR-21-5p Let-7 family, which induces significant changes their cellular seed occupancy components, establishing cancer-specific gene expression profile. D-score, reflecting dominant representative...
Journal Article Mutational Analysis of the Carboxyl-terminal Region SV40 Major Capsid Protein VP1 Get access Naoki Yokoyama, Yokoyama 1 Graduate School Bioscience and Biotechnology, 3 Integrated Research Institute, Tokyo Institute Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; 2 National Longevity Sciences, 36-3 Gengo, Morioka-machi, Obu 474-8511, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Masa-aki Kawano, Kawano Hiroko Tsukamoto,...
The telomere G-tail (G-tail) plays an essential role in maintaining chromosome stability. In this study, we assessed the leukocyte length of breast cancer (BC) patients and cancer-free individuals evaluated association between presence BC. A significant shortening median was observed BC compared with found early phase Our study indicated that might be a potential biomarker for detection.
ABSTRACT Adeno-associated virus (AAV) integrates site specifically into the AAVS1 locus on human chromosome 19. Although recruitment of AAV nonstructural protein Rep78/68 to R ep b inding s ite (RBS) is thought be an essential step, mechanism site-specific integration, particularly, how integration determined, remains largely unknown. Here we describe identification and characterization a new cellular regulator integration. T AR NA loop binding p rotein 185 (TRP-185), previously reported...
Nuclear factor-κB (NF-κB) is an important transcription factor involved in various biological functions, including tumorigenesis. Hence, NF-κB has attracted attention as a target for cancer treatment, leading to the development of several inhibitors. However, existing inhibitors do not discriminate between its subunits, namely, RelA, RelB, cRel, p50, and p52. Conventional methods used evaluate interactions factors DNA, such electrophoretic mobility shift assay luciferase assays, are...