A5058942581- CRISPR and Genetic Engineering
- BRCA gene mutations in cancer
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Reproductive System and Pregnancy
- Genetics, Bioinformatics, and Biomedical Research
- Biotechnology and Related Fields
- Nutrition, Genetics, and Disease
- DNA and Nucleic Acid Chemistry
- Virus-based gene therapy research
- Cancer therapeutics and mechanisms
- Animal Genetics and Reproduction
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- Genomics and Rare Diseases
- Genomics and Chromatin Dynamics
- Phenothiazines and Benzothiazines Synthesis and Activities
- Viral Infectious Diseases and Gene Expression in Insects
- Autophagy in Disease and Therapy
- Fungal and yeast genetics research
- Nuclear Structure and Function
- Cell Adhesion Molecules Research
- Pregnancy and preeclampsia studies
- Hemoglobin structure and function
- Pregnancy and Medication Impact
Tokyo Medical and Dental University
2010-2022
University of the Ryukyus
2019
Tokyo Institute of Technology
1985-2016
Gene Therapy Laboratory
2015
Medical Research Institute
2014
Kyoto Pharmaceutical University
2012
Nara Institute of Science and Technology
2010
Japanese Foundation For Cancer Research
2007-2009
Okayama University
2003-2008
Tokyo Medical University
2007
Abstract Alternative autophagy is an autophagy-related protein 5 (Atg5)-independent type of macroautophagy. Unc51-like kinase 1 (Ulk1) essential initiator not only for Atg5-dependent canonical but also alternative autophagy. However, the mechanism as to how Ulk1 differentially regulates both types has remained unclear. In this study, we identify a phosphorylation site at Ser 746 , which phosphorylated during genotoxic stress-induced Phospho-Ulk1 localizes exclusively on Golgi and required...
ABSTRACT For nuclear entry of large nucleoprotein complexes, it is thought that one key localization signal (NLS) a protein component becomes exposed to mediate importin recognition. We show the simian virus 40 involves dynamic interplay between two distinct interiorly situated capsid NLSs, Vp1 NLS and Vp3 NLS, selective exposure recognition NLS. The NLS-null mutants assembled normally into virion-like particles (VLP) in mutant DNA-transfected cells. When used infect new host, null VLP...
Cytochrome b558 in phagocytes is a transmembrane protein composed of large and small subunits considered to play key role O2- generation during the respiratory burst. The COOH-terminal regions cytochrome protrude cytoplasmic side are assumed be sites for association with cytosolic components form an active O(2-)-generating complex (Imajoh-Ohmi, S., Tokita, K., Ochiai, H., Nakamura, M., Kanegasaki, S. (1992) J. Biol. Chem. 267, 180-184). We show here that two synthetic peptides corresponding...
BRCA2 germline mutations account for the majority of heredity breast and ovarian cancer. Besides its role in DNA damage repair, also plays an important cytokinesis, transcription regulation, cancer cell proliferation. Recently, we reported that localizes to centrosomes as well nuclei dysfunction a centrosome causes abnormalities division. Here, identified nucleolar phosphoprotein, nucleophosmin (NPM), novel BRCA2-associated protein. We detected binding ROCK2, effector Rho small GTPase....
The breast cancer susceptibility gene ( BRCA2 ) is localized mainly in the nucleus where it plays an important role DNA damage repair. Some protein also present centrosome. Here, we demonstrate that interacts with plectin, a cytoskeletal cross‐linker protein, and this interaction controls position of Phosphorylation plectin by cyclin‐dependent kinase 1/cyclin B (CDK1/CycB) has been reported to abolish its cross‐linking function during mitosis. induced phosphorylation prepared fractions HeLa...
Cytokinesis is the critical final step in cell division. BRCA2 disruption during cytokinesis associated with chromosome instability, but mechanistic information lacking that could be used to prevent cancer In this study, we report phosphorylation by mitotic polo-like kinase (PLK1) governs localization of Flemming body at central midbody, permitting an interaction nonmuscle myosin IIC (NM-IIC). Formation NM-IIC ring-like structure shows IIC-ring relies on its ATPase activity stimulated and...
The ability of cancer cells to metastasize is dependent on the interactions between their cell-surface molecules and microenvironment. However, tumor microenvironment, especially cancer-associated stroma, poorly understood. To identify proteins present in we focused phyllodes tumors, rare breast tumors that contain stromal cells. We compared expression normal tissues using an iTRAQ-based quantitative proteomic approach. Decorin was expressed at reduced levels tissues, whereas periostin...
We investigate perovskite/heterojunction crystalline silicon monolithic tandem solar cells by using device simulation. A hydrogenated amorphous and microcrystalline silicon‐based tunnel recombination junction is applied to the cells. The influence of conduction valence band offset between n‐type layer perovskite top cell was investigated. To obtain excellent performance, should be 0–0.6 eV, although does not affect performance. also found that higher fill factor expected when located on...
Abstract Cellular senescence caused by oncogenic stimuli is associated with the development of various age-related pathologies through senescence-associated secretory phenotype (SASP). SASP mediated activation cytoplasmic nucleic acid sensors. However, molecular mechanism underlying accumulation nucleotide ligands in senescent cells unclear. In this study, we revealed that expression RNaseH2A, which removes ribonucleoside monophosphates (rNMPs) from genome, regulated E2F transcription...
On the basis of a self-consistent Einstein model, theoretical treatment for anharmonic lattice vibration metallic fine particles is presented and size dependence melting temperature discussed. The phenomenon related to an instability lattice. A model calculation gives good results in accord with experiment.
FK 506 binding protein 51 ( FKBP 51), a member of the immunophilin family, is involved in multiple signaling pathways, tumorigenesis, and chemoresistance. expression correlates with metastatic potential melanoma prostate cancer. However, functions 51, particularly involving regulation cell motility invasion, are not fully understood. We discovered two novel interacting partner proteins i.e., deleted liver cancer 1 DLC 1) 2 2), using immunoprecipitation mass spectrometry. Rho GTP...
Abstract High estrogen concentration leads to an inflammatory reaction in the mammary gland tissue vivo ; however, detailed mechanism underlying its specific effects on breast duct has not been fully clarified. We used 3D-cultured MCF-10A acini as a model and demonstrated various deleterious of 17-β estradiol (E2), including destruction basement membrane surrounding acini, abnormal adhesion between cells, cell death via apoptosis pyroptosis. Moreover, we clarified these phenomena: E2 binds...
Cytochrome b558 composed of large and small subunits, cytosolic 47- 65-kDa proteins are important constituents the superoxide (O2-) generating system in phagocytes B lymphocytes. In this paper, we describe changes O2- -generating activity expression components during differentiation human monoblastic leukemia U937 cells to macrophage-like cells. Undifferentiated generated no O2-in response a stimulation, although they expressed three other than cytochrome subunit. When were cultured with...
BRCA2 is responsible for familial breast and ovarian cancer has been linked to DNA repair centrosome duplication. Here we analyzed the mechanism by which centrosomal localization signal (CLS) of interacts with cytoplasmic dynein 1 localize centrosome. In vitro pull-down assays demonstrated that directly binds light intermediate chain 2. A dominant-negative HA-CLS-DsRed fusion protein, depletion siRNA, inactivation EHNA, inhibited at centrosomes caused separation pairs during S-phase. The...