A5101694057- Platelet Disorders and Treatments
- Lipid metabolism and disorders
- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Renal Diseases and Glomerulopathies
- Monoclonal and Polyclonal Antibodies Research
- Hematopoietic Stem Cell Transplantation
- Chronic Lymphocytic Leukemia Research
- Pancreatic function and diabetes
- Cardiac Imaging and Diagnostics
- Zebrafish Biomedical Research Applications
- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Cardiovascular Disease and Adiposity
- Erythropoietin and Anemia Treatment
- Cell Adhesion Molecules Research
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Cardiovascular Function and Risk Factors
- Cancer-related molecular mechanisms research
- Renal and related cancers
Waseda University
2015-2024
Japanese Red Cross Nagoya Daiichi Hospital
2009-2023
Maebashi Red Cross Hospital
2022-2023
Dokkyo Medical University
2020-2022
Gifu Prefectural General Medical Center
2011-2022
Toyokawa City Hospital
2022
Meiji University
1995-2022
Nagoya Ekisaikai Hospital
2018-2022
Okinawa Prefectural Chubu Hospital
2021
Kudanzaka Hospital
2020-2021
Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although potential as cancer biomarkers has been promising, the identification quantification of EVs in clinical samples remains challenging. Here we describe a sensitive rapid analytical technique for profiling circulating directly from blood patients with colorectal cancer. are captured by two types antibodies detected photosensitizer-beads, which enables us to detect...
Several cell types, including tumour cells, secrete extracellular vesicles (EVs), and tumour-derived EVs play a role in cancer initiation progression. These include both common set of membrane cytosolic proteins origin-specific subsets that likely correlated to type-associated functions. To confirm the presence preparations, researchers have identified so-called EV marker proteins, tetraspanin family such as heat shock 70 kDa protein 4 (HSP70) susceptibility gene 101 (TSG101). However,...
Silencing of gene expression by small interfering RNAs (siRNAs) is rapidly becoming a powerful tool for genetic analysis and represents potential strategy therapeutic product development. However, there are no reports systemic delivery siRNAs toward treatment bone-metastatic cancer. Accordingly, we report here that i.v. injection GL3 luciferase siRNA complexed with atelocollagen showed effective reduction from prostate tumor cells developed in mouse thorax, jaws, and/or legs. We also show...
Abstract Mesenchymal stem cells (MSCs), largely present in the adult human body, represent an attractive tool for establishment of a cell-based therapy liver diseases. Recently, therapeutic potential and immunomodulatory activity MSCs have been revealed. Adipose tissue-derived mesenchymal (AT-MSCs), so-called adipose-derived or adipose stromal cells, because their high accessibility with minimal invasiveness, are especially context future clinical applications. The goal study was to evaluate...
The asparagine-linked sugar chains of human erythropoietin produced by recombinant Chinese hamster ovary cells and naturally occurring urinary were liberated hydrazinolysis fractionated paper electrophoresis, lectin affinity chromatography, Bio-Gel P-4 column chromatography. Both erythropoietins had three in one molecule, all which acidic complex type. Structural analysis them revealed that the from both are quite similar except for sialyl linkage. All contain only NeuAc alpha 2----3Gal...
Abstract Background and Aim: Multipotential mesenchymal stem cells (MSC), present in many organs tissues, represent an attractive tool for the establishment of a successful cell‐based therapy field regeneration medicine. Adipose tissue (AT‐MSC), known as adipose‐derived (ASC) are especially context future clinical applications because their high accessibility minimal invasiveness during procedure to obtain them. The goal study was induce human ASC into functional hepatocytes vitro within...
A thrombopoietic factor, termed thrombopoietin (TPO), was highly purified directly from the plasma of sublethally irradiated 1,100 rats by measuring production megakaryocytes a enriched population rat megakaryocyte progenitor cells (CFU-MK). The TPO is glycoprotein and strongly hydrophobic. total activity purification yields obtained were about 29% 1.49 x 10(8), respectively. amino acid sequences two peptide fragments prepared 19 kDa analyzed, used for cloning human cDNAs. It found that...
The complete gene for human thrombopoietin (TPO) has been cloned by screening a genomic library using TPO cDNA as probe. This is 6.2 kb in length and contains six exons five introns. It shown that the genome single copy of according to Southern blotting analysis. transcription initiation site was determined S1 nuclease mapping. expressed activity when transfected into COS-1 cells. mapped chromosome 3q27 situ hybridization biotin-labeled
The circulating thrombopoietin (TPO) level in 43 patients with chronic immune thrombocytopenic purpura (ITP) was examined by an ELISA system. TPO (mean +/- SD) ITP mildly elevated (1.86 1.17 fmol/ml) compared to that normal subjects (0.76 0.21), and within the range 30% of patients. In contrast, aplastic anaemia very high, 12.35 6.42 fmol/ml. There no correlation between platelet count Splenectomy performed two patients, after which counts increased levels showed a transient increase. These...
A sensitive sandwich enzyme‐linked immunosorbent assay (ELISA) has been established to estimate serum thrombopoietin (TPO) concentrations in healthy volunteers and patients with haemopoietic disorders. The ELISA uses a mouse monoclonal antibody (Ab) as the capture Ab biotinylated rabbit polyclonal detector. was reproducible, highly specific for human TPO. coefficients of intra‐ inter‐assay variation were from 3.0% 4.9% 5.9% 6.1%, respectively. quantitative limit 0.09 fmol/ml serum. lower...
We identified that microRNA expression changed dynamically during liver development and found miR-500 is an oncofetal miRNA in cancer. was abundantly expressed several human cancer cell lines 45% of hepatocellular carcinoma (HCC) tissue. Most importantly, increased amount the sera HCC patients. In fact, levels patients returned to normal after surgical treatment three Our findings reveal diverse changes miRNAs occur and, one these, relevant diagnosis HCC.
The present study shows that recombinant human megakaryocyte growth and development factor (r-HuMGDF) behaves both as a colony stimulating differentiation in progenitor cell cultures. Megakaryocyte formation induced with r-HuMGDF is synergistically affected by stem but not interleukin 3. Megakaryocytes stimulated demonstrate progressive cytoplasmic nuclear maturation. Measurable levels of serum from patients undergoing myeloablative therapy transplantation are shown to be elaborated response...
Although thrombopoietin (TPO) is known to play a fundamental role in both megakaryopoiesis and thrombopoiesis, the molecular mechanism of TPO-induced megakaryocytic differentiation not known. In human megakaryoblastic leukemia cell line, CMK, that showed some degree after culture with TPO, cyclin-dependent kinase (Cdk) inhibitor p21WAF1/Cip1, but p27Kip1, p16INK4A, p15INK4B, or p18INK4C, was found be upregulated an immediately early response TPO. The expression p21 sustained over period 5...
The IkappaB kinase (IKK)-related NAK (also known as TBK or T2K) contributes to the activation of NF-kappaB-dependent gene expression. Here we identify NAP1 (for NAK-associated protein 1), a that interacts with and its relative IKK epsilon IKKi). activates facilitates oligomerization. Interestingly, NAK-NAP1 complex itself effectively phosphorylated serine 536 p65/RelA subunit NF-kappaB, this activity was stimulated by tumor necrosis factor alpha (TNF-alpha). Overexpression specifically...
SHPS-1 is a receptor-type glycoprotein that binds and activates the protein-tyrosine phosphatases SHP-1 SHP-2, thereby negatively modulates intracellular signaling initiated by various cell surface receptors coupled to tyrosine kinases. also regulates intercellular communication in neural immune systems through its association with CD47 (integrin-associated protein) on adjacent cells. Furthermore, recent studies fibroblasts derived from mice expressing an mutant lacks most of cytoplasmic...
Abstract The distribution of reactive astrocytes was examined in the cortical gray matter non‐motor and motor regions from cases familial sporadic amyotrophic lateral sclerosis (ALS) compared to that β‐amyloid deposits. By glial fibrillary acidic protein immunocytochemistry, patches astrocytes, characterized by multiple a circular or patch‐like formation, occurred 12 15 ALS examined. These were not restricted cortex but found all brain regions, including frontal, temporal, inferior parietal,...
A simple and rapid method was developed for the determination of 3,4-dihydroxyphenylalanine (dopa) 5-S-cysteinyl-3,4-dihydroxyphenylalanine (5-S-cysteinyldopa) in proteins with use high-pressure liquid chromatography. With this method, it is demonstrated that mushroom tyrosinase can catalyse hydroxylation tyrosine residues to dopa subsequent oxidation dopaquinone residues. The combine cysteine form 5-S-cysteinyldopa bovine serum albumin yeast alcohol dehydrogenase, whereas major product...
Abstract Background Acquisition of drug-resistance in cancer has led to treatment failure, however, their mechanisms have not been clarified yet. Recent observations indicated that aberrant expressed microRNA (miRNA) caused by chromosomal alterations play a critical role the initiation and progression cancer. Here, we performed an integrated genomic analysis combined with array-based comparative hybridization, miRNA, gene expression microarray elucidate mechanism drug-resistance. Results...
Iron is fundamental for sustaining life living organisms, and the iron metabolism finely regulated at different levels. In cancer cells, deregulation of induces oxidative stress drives tumor progression metastasis; however, molecular mechanisms homeostasis are not fully understood. Here we found that deficiency as well hypoxia promoted microRNA-210 (miR-210) expression. A central mediator miR-210 transcriptional activation hypoxia-inducible factor (HIF)-1α, hypoxia-response element in...