A5103278237- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Gastric Cancer Management and Outcomes
- Esophageal Cancer Research and Treatment
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Gastrointestinal Tumor Research and Treatment
- Glycosylation and Glycoproteins Research
- Esophageal and GI Pathology
- Metastasis and carcinoma case studies
- HER2/EGFR in Cancer Research
- T-cell and B-cell Immunology
- Fibroblast Growth Factor Research
- Lung Cancer Treatments and Mutations
- Colorectal Cancer Surgical Treatments
- Colorectal and Anal Carcinomas
- CAR-T cell therapy research
- Helicobacter pylori-related gastroenterology studies
- Cancer Cells and Metastasis
- Peptidase Inhibition and Analysis
- Inflammatory Biomarkers in Disease Prognosis
- PI3K/AKT/mTOR signaling in cancer
Fukushima Medical University
2016-2025
National University of Singapore
2012-2023
Ohta Nishinouchi Hospital
2023
Pediatrics and Genetics
2023
Target (United States)
2022
Naha City Hospital
2022
University of Aizu
2022
National University Cancer Institute, Singapore
2012-2019
University of Yamanashi
2007-2018
Medical Technologies (Czechia)
2018
Accumulating evidence suggests that exogenous cellular stress induces PD-L1 upregulation in cancer. A DNA double-strand break (DSB) is the most critical type of genotoxic stress, but involvement DSB repair expression has not been investigated. Here we show cancer cells upregulated response to DSBs. This requires ATM/ATR/Chk1 kinases. Using an siRNA library targeting genes, discover BRCA2 depletion enhances Chk1-dependent after X-rays or PARP inhibition. In addition, Ku70/80 substantially...
It has been reported that an increased population of regulatory T cells (Tregs) is one the reasons for impaired anti-tumor immunity. Recently, Foxp3 as a reliable marker Tregs. The authors investigated frequency Foxp3(+) Tregs within CD4(+) in TILs, regional lymph nodes and PBLs gastric cancer patients (n = 45). Furthermore, to elucidate mechanisms behind Treg accumulation tumors, they evaluated relationship between CCL17 or CCL22 expression cancer. CD4(+)CD25(+)Foxp3(+) percentage were...
Abstract Natural killer (NK) cells rely on surface receptors to distinguish healthy from cancer cells. We designed a receptor termed NKG2D-DAP10-CD3ζ that is composed of the NK cell activating molecule NKG2D plus 2 key signaling molecules, DAP10 and CD3ζ, evaluated its capacity promote killing. Retroviral transduction markedly increased expression in cells, which became consistently more cytotoxic than mock-transduced against leukemia solid tumor lines. In contrast, there was no increase...
Despite multidisciplinary treatment for patients with advanced gastric cancer, their prognosis remains poor. Therefore, the development of novel therapeutic strategies is urgently needed, and immunotherapy utilizing anti‐programmed death 1/‐programmed ligand‐1 mA b an attractive approach. However, as there limited information on how programmed upregulated tumor cells within microenvironment, we examined mechanism regulation a particular focus interferon gamma in vitro setting clinical...
After publication of the guidelines, Committee on Guidelines for Diagnosis and Treatment Esophageal Cancer Japan Society has taken initiative to continue review contents guidelines conduct public relations dissemination/utilization activities.A revision is planned approximately 5 years after this guideline.In addition, a prompt report will be made when results clinical study(ies) are published or in accordance with changes medical circumstances, such as health insurance coverage.
Abstract Although alterations in CD3‐associated signal‐transducing molecules tumor‐infiltrating T cells of patients with advanced cancer have been previously described, the mechanism behind these changes is not known. We demonstrate that macrophages isolated from metastatic lymph nodes malignant melanoma down‐regulate levels CD3ζ autologous peripheral blood cells. Lipopolysaccharide (LPS)‐ or phorbol 12‐myristate 13‐acetate (PMA)‐stimulated monocytes derived healthy donors also induced...
Overexpression of epidermal growth factor receptor (EGFR) is observed in many cancers, sometimes accompanied by gene amplification. Recently, several clinical therapies targeting EGFR were developed, but the eligibility criteria for these not fully established. To develop such esophageal squamous cell carcinoma (ESCC), we sought to clarify: (i) exact frequency overexpression, (ii) relationship between protein overexpression and amplification, (iii) amplification specific mutations (iv)...
Although it has been shown that chemoradiotherapy may induce immunogenic cell death, which could trigger T-cell immunity mediated by high-mobility group box 1 protein (HMGB1) and calreticulin, there is still limited information to support this theory directly in a clinical setting. In the present study, we evaluated antigen-specific responses against six cancer-testis antigens peripheral blood lymphocytes from patients with esophageal squamous carcinoma (ESCC) receiving chemoradiation....
Diabetic nephropathy is a major risk of end-stage kidney disease. Many complex factors relate to the progression diabetic nephropathy. Using nonobese type 2 diabetes model rats, we confirmed that oxidative stress was crucial factor. Because recent studies suggest vitamin D could suppress stress, explored whether active analog, maxacalcitol, also attenuate and prevent rats aged 20 weeks were divided into 3 groups treated with insulin, vehicle. At age 30 weeks, blood urine analyses, renal...
Abstract c‐ erb ‐2 amplification and overexpression are currently attracting a great deal of attention because new adjuvant therapy using an antibody against the B‐2 gene product, trastuzumab (Herceptin; Genentech, Inc., South San Francisco, CA), has proved effective in treating breast cancer with and/or B‐2. Aberrations have also been detected ovarian, endometrial gastric carcinomas at varied frequencies. Amplification locus (17q12‐q21.32), protein (p185) serum levels soluble fragments...
To expand applications for T-cell-based immunotherapy in cancer, we designed a receptor that binds the Fc portion of human immunoglobulins and delivers activation signals. The construct included high-affinity CD16 (FCGR3A) V158 variant, CD8α hinge, transmembrane domains, along with signaling domains from CD3ζ 4-1BB (TNFRSF9), forming chimeric termed CD16V-BB-ζ. After retrovirus-mediated expression T cells, CD16V-BB-ζ bound humanized antibodies higher affinity than control containing more...
Abstract To evaluate the possibility of treatment with antiepidermal growth factor receptor (EGFR) mAb, Cetuximab against esophageal squamous cell carcinoma (SCC), we performed detail analysis antibody‐dependent cellular cytotoxicity (ADCC) mediated by SCC. Esophageal SCC lines various levels EGFR ( n = 8) were evaluated for their Cetuximab‐mediated ADCC 51 Cr‐release assay. As a result, was able to induce EGFR‐expressing and activities reflected degree expression on The patients' PBMC...
Although Th17 cells reportedly play critical roles in the development of autoimmunity and allergic reactions, information on cancer-bearing hosts is still limited. In present study, we investigated distribution relation to regulatory T (Treg) tumor-infiltrating lymphocytes (TILs), regional lymph node lymphocytes, peripheral blood gastric cancer patients. Interleukin (IL)-17-producing CD4(+) as CD4(+)CD25(+)FoxP3(+) Treg were evaluated by flow cytometry expressed a percentage total cells,...
Since a phase I clinical trial using three HLA-A24-binding peptides from TTK protein kinase (TTK), lymphocyte antigen-6 complex locus K (LY6K), and insulin-like growth factor-II mRNA binding protein-3 (IMP3) had been shown to be promising for esophageal squamous cell carcinoma (ESCC), we further performed multicenter, non-randomized II trial. Sixty ESCC patients were enrolled evaluate OS, PFS, immunological response employing ELISPOT pentamer assays. Each of the was administered with IFA...
We previously identified three novel HLA‐A24‐restricted epitope peptides, which were derived from cancer‐testis antigens, TTK protein kinase (TTK), lymphocyte antigen 6 complex locus K (LY6K), and insulin‐like growth factor (IGF)‐II mRNA binding 3 (IMP‐3), as targets for cancer vaccination against esophageal squamous cell carcinoma (ESCC). To examine the safety, immunogenicity, antitumor effect of vaccine treatment using a combination these 10 HLA‐A2402‐positive advanced ESCC patients who...
Purpose There is growing evidence that tumor-specific immune responses play an important role in anti-cancer therapy, including radiotherapy. Using mouse tumor models we demonstrate irradiation-induced anti-tumor immunity essential for the therapeutic efficacy of irradiation and can be augmented by modulation cytotoxic T lymphocyte (CTL) activity. Methods Materials C57BL/6 mice, syngeneic EL4 lymphoma cells, Lewis lung carcinoma (LL/C) cells were used. Cells injected into right femurs mice....
Abstract Immunotherapy against the interaction between programmed cell death 1/programmed ligand 1 (PD-L1) has emerged as a promising strategy for colorectal cancer with mismatch repair deficiency (dMMR) or microsatellite instability-high (MSI-H). The study aimed to identify miRNAs that posttranscriptionally control PD-L1 expression on tumor cells and also regulate immune evasion. A comprehensive miRNA screening using Cancer Genome Atlas (TCGA) dataset (n = 260) combined eight different...
Annexin A1 (ANXA1) is a phospholipid-linked protein involved in inflammation, immune response, and mast cell reactivity. Recently, we reported that ANXA1 associated with aggressive features of triple-negative breast cancer (TNBC); however, its clinical relevance remains controversial. We hypothesized human TNBC high expression mRNA pro-cancerous infiltration, including cells, an phenotype. Clinical RNA-seq data were obtained from The Cancer Genome Atlas (TCGA, n = 1079) Molecular Taxonomy...