A5052200323- Pregnancy-related medical research
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive System and Pregnancy
- Adipose Tissue and Metabolism
- Assisted Reproductive Technology and Twin Pregnancy
- Regulation of Appetite and Obesity
- Heat shock proteins research
- Testicular diseases and treatments
- Prenatal Screening and Diagnostics
- Cell death mechanisms and regulation
- Ectopic Pregnancy Diagnosis and Management
- Immune Cell Function and Interaction
- Pluripotent Stem Cells Research
- Renal and related cancers
- Pregnancy and preeclampsia studies
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Insurance, Mortality, Demography, Risk Management
- Maternal and fetal healthcare
- Adipokines, Inflammation, and Metabolic Diseases
- Ovarian cancer diagnosis and treatment
- Psoriasis: Treatment and Pathogenesis
- Nerve injury and regeneration
- Dietary Effects on Health
Chiba University
2016-2023
Iwate Medical University
2018-2020
Chiba University Hospital
2018
Akita University
2006-2017
Chiba Aoba Municipal Hospital
2013
Stanford University
2002-2005
Florey Institute of Neuroscience and Mental Health
2004
The University of Melbourne
2004
University of Turku
2004
Relaxin is a hormone important for the growth and remodeling of reproductive other tissues during pregnancy. Although binding sites relaxin are widely distributed, nature its receptor has been elusive. Here, we demonstrate that two orphan heterotrimeric guanine nucleotide protein (G protein)–coupled receptors, LGR7 LGR8, capable mediating action through an adenosine 3′,5′-monophosphate (cAMP)–dependent pathway distinct from structurally related insulin insulin-like factor family ligand....
Several orphan G protein-coupled receptors homologous to gonadotropin and thyrotropin have recently been identified named as LGR4-8. INSL3, also known Leydig insulin-like peptide or relaxin-like factor, is a relaxin family member expressed in testis cells ovarian theca luteal cells. Male mice mutant for INSL3 exhibit cryptorchidism defects descent due abnormal gubernaculum development whereas overexpression of induces ovary transgenic females. Because missing the LGR8 gene are cryptorchid,...
Mammalian oocytes are arrested at the prophase of meiosis before induction maturation by preovulatory luteinizing hormone (LH) surge. LH also promotes survival meiotic male germ cells in testis. Because binds somatic cells, mechanism underlying its regulation cell function is unclear. We found that stimulates Leydig insulin-like 3 (INSL3) transcripts ovarian theca and testicular cells. INSL3, turn, a G protein-coupled receptor, LGR8 (leucine-rich repeat-containing receptor 8), expressed to...
Leucine-rich repeat-containing, G protein-coupled receptors (LGRs) represent a unique subgroup of with large ectodomain. Recent studies demonstrated that relaxin activates two orphan LGRs, LGR7 and LGR8, whereas INSL3/Leydig insulin-like peptide specifically LGR8. Human 3 (H3 relaxin) was recently discovered as novel ligand for receptors. Here, we demonstrate H3 but not Taking advantage the overlapping specificity these three ligands related chimeric were generated to elucidate mechanism...
The present study was designed to clarify the role of radical oxygen species in testicular germ cell apoptosis induced by heat stress. Testicular cells isolated from immature rats were cultured with or without elevated temperature, and occurrence these defined appearance DNA fragmentation following agarose gel electrophoresis flow cytometric quantification apoptotic cells. At 32.5°C, < 1% showed signs throughout culture period, whereas under stress, proportion increased 5% at 37°C after 24 h...
Although ghrelin acts as a modulator of feeding behavior and energy metabolism in the central nervous system, recent studies have implicated peripheral actions reproductive tissues. Here, we investigated expression its receptor (GHS-R) mouse oocyte preimplantation embryos, examined role regulation early embryo development. Both GHS-R mRNAs were detected morula or more advanced stages. As for origin ghrelin, both mRNA protein identified uterine endometrium. The levels fluid well plasma...
Leptin acts as a modulator of diverse reproductive functions, and recent studies have implicated involvement leptin in the early embryo development mammal. The aim this study was to investigate expression its receptor (OB-R) mouse oocyte preimplantation embryo, examine whether influenced development. mRNA detected blastocyst hatched blastocyst, two splice variants OB-R (OB-Ra OB-Rb) mRNAs were oocytes, 1-cell, 2-cell, morula, blastocyst. As for origin leptin, both protein identified oviduct...
Successful implantation of the blastocyst and subsequent placental development is essential for reproduction. Expression brain-derived neurotrophic factor (BDNF) neurotrophin-4/5, together with their receptor, tyrosine kinase B (TrkB), in trophectoderm cells suggests potential roles development. Here we demonstrated that treatment BDNF promoted outgrowth, but not adhesion, vitro increased levels cell invasion marker matrix metalloproteinase-9 cultured blastocysts through phosphatidylinositol...
Relaxin family peptide 1 (RXFP1) receptor (LGR7) and RXFP2 (LGR8) were recently identified as the targets for H2 relaxin insulin-like 3 (INSL3), respectively. In this study, we define pharmacology of these two receptors by using a number chimeras peptides. We have binding sites on receptors: one primary, high-affinity site within ectodomain secondary, lower affinity transmembrane region. The primary was found to dictate characteristics, although also exerts some influence modulates ligand in...
Lynch syndrome (LS) is an autosomal-dominant inherited disorder mainly caused by a germline mutation in the DNA mismatch repair (MMR) genes (MLH1, MSH2, MSH6, and PMS2) associated with increased risk for various cancers, particularly colorectal cancer endometrial (EC). Women LS account 2% to 6% of EC patients; it clinically important identify such individuals predicting and/or preventing additional LS-associated cancers. PMS2 (PMS2-LS) rarest contribution etiology among 4 MMR mutations, its...
Although medical treatment of unruptured ectopic pregnancy using methotrexate has been established, development more potent and safer is needed due to limited indications side effects methotrexate. Brain-derived neurotrophic factor (BDNF) signals through its receptor tyrosine kinase B (TrkB) regulate the growth malignant trophoblastic, choriocarcinoma cell. We investigated possible involvement this signaling system in nonmalignant human trophoblast both intrauterine pregnancy. Here, we...
Does a new system—the chip-sensing embryo respiration monitoring system (CERMs)—enable evaluation of viability for potential application in clinical IVF setting? The enabled the oxygen consumption rate spheroids, bovine embryos and frozen-thawed human to be measured, this corresponded developmental embryos. To date, no reliable clinically suitable objective methods are available, which circumvent differences inter-observer subjective view. Existing systems such as scanning electrochemical...
Abstract The nutritional curcumin (CUR) is beneficial in cell-mediated autoimmune diseases. molecular mechanisms underlying this food-mediated silencing of inflammatory immune responses are poorly understood. By investigating antigen-specific we found that dietary CUR impairs the differentiation Th1/Th17 cells vivo during encephalomyelitis and instead promoted Th2 cells. In contrast, feeding had no inhibitory effect on ovalbumin-induced airway inflammation. Mechanistically, induces an...
Abstract The lung develops an unique epithelial barrier system to protect host from continuous invasion of various harmful particles. Interleukin (IL-)33 released cells in the drives type 2 immune response by activating ST2− expressed allergic diseases. However, involvement memory-type ST2 + CD4 T such inflammation remains unclear. Here we demonstrated that intratracheal administration IL-33 resulted substantial increase numbers tissue-resident lung. Following enhanced production IL-5 and...
Survival and apoptosis of cells in preimplantation embryos are fundamental for successful pregnancy. Relevant to these processes, tumor necrosis factor (TNF) transforming growth alpha (TGFA) produced by mammalian oviducts uteri. In early embryos, TNF induces apoptosis, whereas TGFA could act as a survival factor. Here we investigated the regulation mouse its antagonism TGFA. receptor superfamily, member 1a mRNA was detectable throughout embryonic stages, with an increase after blastocyst...
Uncontrolled type 2 immunity by helper T (Th2) cells causes intractable allergic diseases; however, whether the interaction of CD4 + shapes pathophysiology diseases remains unclear. We identified a subset Th2 that produced serine proteases granzyme A and B early in differentiation. Granzymes cleave protease-activated receptor (Par)-1 induce phosphorylation p38 mitogen-activated protein kinase (MAPK), resulting enhanced production IL-5 IL-13 both mouse human cells. Ubiquitin-specific protease...
Abstract Purpose In microscopic testicular sperm extraction (mTESE) for nonobstructive azoospermia (NOA), can be recovered relatively easily in some cases, and mTESE may retrospectively considered excessive. However, is routinely performed the majority of NOA patients because difficulty predicting tissue status. A minimally invasive comprehensive retrieval method that allows on‐the‐spot assessment needed. We have developed evaluated a novel technique called micromapping (MMTSE). Methods...
Both GnRH-I and its receptor (GnRHR)-I have been shown to be expressed in the mammalian preimplantation embryo. In this study, we investigated molecular mechanisms of regulation early embryonic development mouse. We found that GnRHR-I mRNAs were detectable throughout stages expression levels both increased significantly after blastocyst stage. blastocysts, was detected inner cell mass trophectoderm cells. The pregnant uterus also genes, suggesting embryos could affected by GnRH through...
Women with a history of gestational diabetes mellitus (GDM) are likely to develop postpartum (DM). We examined women in the early stages pregnancy who were at high risk DM progression establish follow-up method for detection.We performed oral glucose tolerance test (OGTT) and identified predictive factors impaired (IGT) or 77 after GDM, 2 years delivery, retrospectively. Cutoff values each factor determined. classified these GDM into four groups using evaluated intolerance (GI) group.In...