A5087689490- Protist diversity and phylogeny
- Autophagy in Disease and Therapy
- Signaling Pathways in Disease
- Glycosylation and Glycoproteins Research
- Protein Kinase Regulation and GTPase Signaling
- Ubiquitin and proteasome pathways
- interferon and immune responses
- PI3K/AKT/mTOR signaling in cancer
- Peptidase Inhibition and Analysis
- Microbial Community Ecology and Physiology
- Calcium signaling and nucleotide metabolism
- Protein Degradation and Inhibitors
- Marine Biology and Ecology Research
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Cellular Mechanics and Interactions
- Protein Tyrosine Phosphatases
- Influenza Virus Research Studies
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Pancreatic and Hepatic Oncology Research
- Cellular transport and secretion
- Cytokine Signaling Pathways and Interactions
Kagawa Prefectural College of Health Sciences
2024-2025
Kagawa University
2022-2025
Hokkaido University
2013-2022
Harvard University
2003-2021
Translational Therapeutics (United States)
2021
Beth Israel Deaconess Medical Center
2003-2021
Cancer Research Institute
2021
Koen Gakuen Women's Junior College
2008
Daido University
2007
Hiroshima University
1996-2002
The possibility of the pandemic spread influenza viruses highlights need for an effective cure this life-threatening disease. Influenza A virus, belonging to a family orthomyxoviruses, is negative-strand RNA virus which encodes 11 viral proteins. numbers intracellular signaling pathways in host cells interact with proteins, affect various stages infection and replication. In study, we investigated how inhibition Akt kinase activity impacts on by using "Akt-in", peptide inhibitor. PR8...
EZH2 inhibition and reactivation of tumor suppressor microRNAs (miRNAs) represent attractive anti-cancer therapeutic strategies. We found that EZH2-suppressed let 7b miR-361, two likely suppressors, inhibited endometrial cancer (EC) cell proliferation invasion, abrogated stem cell-like properties. In EC cells, induced functioned together with YY1 to epigenetically suppress which upregulated Twist, a direct target miR-361. Treating cells GSK343, specific inhibitor, mimicked the effects...
Background and Aims Current treatment with nucleos(t)ide analogs (NUCs) safely controls the replication of hepatitis B virus (HBV) improves prognosis in patients HBV. However, inability to completely clear HBV is problematic, novel therapies are desired. It has been believed that all NUCs have similar functions inhibit reverse transcriptase. our recent findings only acyclic nucleoside phosphonates (ANPs; adefovir dipivoxil tenofovir disoproxil fumarate) had an additional effect inducing...
A novel myosin light chain kinase (MLCK) cDNA was isolated from a HeLa cell library. The deduced amino acid sequence identical to that of zipper‐interacting protein (ZIPK) which mediates apoptosis [Kawai et al. (1998) Mol. Cell. Biol. 18, 1642–1651]. Here we found ZIPK phosphorylated the regulatory II (MRLC) at both serine 19 and threonine 18 in Ca 2+ /calmodulin independent manner. Phosphorylation by resulted activation actin‐activated MgATPase activity II. is first non‐muscle MLCK...
Phosphorylation on Ser/Thr-Pro motifs is a major mechanism regulating many events involved in cell proliferation and transformation, including centrosome duplication, whose defects have been implicated oncogenesis. Certain phosphorylated can exist two distinct conformations conversion certain proteins catalyzed specifically by the prolyl isomerase Pin1. Pin1 prevalently overexpressed human cancers important for activation of multiple oncogenic pathways, its deletion suppresses ability...
Primary Sjögren's syndrome (pSS) is a complex autoimmune disease characterized by dysfunction of secretory epithelia with only palliative therapy. Patients present constellation symptoms, and the diversity symptomatic presentation has made it difficult to understand underlying mechanisms. In this study, aggregation unbiased transcriptome profiling data sets minor salivary gland biopsies from controls patients identified increased expression lysosome-associated membrane protein 3...
Article24 May 2016Open Access Transparent process Phosphorylation-dependent Akt–Inversin interaction at the basal body of primary cilia Futoshi Suizu Division Cancer Biology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan Search more papers by this author Noriyuki Hirata Kohki Kimura Tatsuma Edamura Tsutomu Tanaka Satoko Ishigaki Thoria Donia Chemistry Department, Faculty Science, Tanta Tanta, Egypt Hiroko Noguchi Department Pathology, Teine Keijinkai Hospital, Toshihiko...
Autophagy is an evolutionarily conserved mechanism for the gross disposal of intracellular proteins in mammalian cells and dysfunction this pathway has been associated with human disease. Although serine threonine kinase Akt suggested to play a role process, little known about molecular mechanisms by which induces autophagy. Using yeast two-hybrid screen, Phafin2 (EAPF or PLEKHF2), lysosomal protein unique structure N-terminal PH (pleckstrin homology) domain C-terminal FYVE (Fab 1, YOTB, Vac...
The formation of resting cysts is a strategy for survival in unfavorable environments by single cell organisms such as protists. Here, we show that Colpoda have high salinity tolerance and investigate the changes gene expression underpin this effect. can tolerate saline conditions up to 3.5 % NaCl. A comparative transcriptome analysis vegetative cells showed relative levels genes associated with membrane function increased cysts. These suggest reconstruction plasma tolerance. cyst forms...
The dense stroma is one cause of poor efficacy T cell-mediated immunotherapy in pancreatic ductal adenocarcinoma (PDAC). Carbohydrate sulfotransferase 15 (CHST15) a proteoglycan-synthetic enzyme responsible for remodeling tumor stroma. Intra-tumoral injection CHST15 small interfering RNA (siRNA) has been shown to increase the tumor-infiltrating cells (TILs) patients with unresectable PDAC. However, mechanism underlying enhanced accumulation TILs not fully explored. Here, we demonstrate that...
Serine-threonine kinase Akt (also known as PKB, protein B), a core intracellular mediator of cell survival, is involved in various human cancers and has been suggested to play an important role the regulation autophagy mammalian cells. Nonetheless, physiological function lysosomes currently unknown. We have reported previously that PtdIns(3)P-dependent lysosomal accumulation Akt-Phafin2 complex critical step for induction. Here, characterize molecular activated process autophagy, we searched...
Ca2+/calmodulin (CaM)-dependent protein kinase I (CaM-KI), which is a member of the multifunctional CaM-K family, thought to be involved in various Ca2+-signalling pathways. In this report, we demonstrate that CaM-KI activated by an upstream (CaM-K kinase), but not unactivated CaM-KI, phosphorylates myosin II regulatory light chain (MRLC) efficiently (Kcat, 1.7s-1) and stoichiometrically (0.8mol phosphate/mol) Ca2+/CaM-dependent manner vitro. One-dimensional phosphopeptide mapping...
Abstract Background Telomere dysfunction has been reported to be directly involved in carcinogenesis owing chromosomal instability and immortalization; however, the clinicopathological significance of telomeres remains controversial. We have shown that telomere shortening occurs normal-appearing duct cells at initiation then continues during progression pancreatic cancer. In this study, we determined prognostic value length (TL) cancer progression. Methods TL both cancer-associated...
Journal Article Activation of Action-Activated MgATPase Activity Myosin II by Phyosphorylation with MAPK-Activated Protein Kinase-1b (RSK-2) Get access Futoshi Suizu, Suizu Department Biological Science, Graduate School Hiroshima University Higashi-HiroshimaHigashi-Hiroshima 739-8526 Search for other works this author on: Oxford Academic PubMed Google Scholar Kozue Ueda, Ueda Takahiro Iwasaki, Iwasaki Maki Murata-Hori, Murata-Hori Hiroshi Hosoya 2 2To whom correspondence should be...
Abstract Protooncogene T-cell leukemia 1 (TCL1), which is implicated in human prolymphocytic (T-PLL), interacts with Akt and enhances its kinase activity, functioning as an co-activator. Two major isoforms of TCL1 Protooncogenes ( TCL1b ) are present adjacent to each other on chromosome 14q.32. In T-PLL, both activated by chromosomal translocation. Moreover, TCL1b-transgenic mice have never been created. Therefore, it remains unclear whether itself, independent TCL1, exhibits oncogenicity....