A5023485577- Salivary Gland Disorders and Functions
- Galectins and Cancer Biology
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Bladder and Urothelial Cancer Treatments
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- Gastric Cancer Management and Outcomes
- Immune cells in cancer
- Urinary and Genital Oncology Studies
- Sarcoma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Lymphatic System and Diseases
- Prostate Cancer Diagnosis and Treatment
- Esophageal Cancer Research and Treatment
- Prostate Cancer Treatment and Research
- interferon and immune responses
- Cellular transport and secretion
- Virus-based gene therapy research
- Calcium signaling and nucleotide metabolism
- Immune Response and Inflammation
- Esophageal and GI Pathology
- Seed and Plant Biochemistry
- Testicular diseases and treatments
National Institute of Dental and Craniofacial Research
2019-2024
National Institutes of Health
2019-2024
Hokkaido University
1990-2024
Aichi Cancer Center
2024
The Nakamura Hajime Eastern Institute
2023
Association of American Veterinary Medical Colleges
2021-2022
Sapporo Medical University
2007-2017
Yamaguchi University
2015-2017
Okayama University
2009
Kyoto University
2006
A253-control: A253 control for LAMP3 stable overexpression; A253- LAMP3: LAPM3 CASP1: caspase 1; CASP3: 3; CHX: cycloheximide; CTSB: cathepsin B; CTSD: D; CQ: chloroquine; DCs: dendritic cells; ER: endoplasmic reticulum; LGALS3: galectin HCV: hepatitis C virus; HSG-control: HSG HSG-LAMP3: HSP: heat shock protein; HTLV-1: human T-lymphocyte leukemia virus-1; IXA: ixazomib; LAMP: lysosomal associated membrane MHC: major histocompatibility complex; mAb: monoclonal antibody; OE: pepA: pepstatin...
Human endoplasmic reticulum oxidoreductin 1-α (hERO1-α) is an oxidizing enzyme that exists in the and its expression augmented under hypoxia. It regulates a redox state of various kinds protein through reoxidation "client" disulfide isomerase. Interestingly, although hERO1-α normal tissues was comparatively limited, types cancer cells expressed it large amounts. Therefore, we examined role ERO1-α tumor growth using murine breast line 4T1 found knockdown inhibited vivo decreased lung...
Many human cancers have been reported to enhanced expression of the immune checkpoint molecule programmed death-ligand 1 (PD-L1), which binds cell death-1 (PD-1) expressed on cells. PD-L1/PD-1 plays a role in inhibition antitumor immunity by inducing T apoptosis and tolerance. Thus, it is crucial elucidate mechanisms PD-L1 cancer ERO1-α an oxidase located endoplasmic reticulum. It overexpressed variety tumor types disulfide bond formation collaboration with PDI. Here, we investigated...
Primary Sjögren's syndrome (pSS) is a complex autoimmune disease characterized by dysfunction of secretory epithelia with only palliative therapy. Patients present constellation symptoms, and the diversity symptomatic presentation has made it difficult to understand underlying mechanisms. In this study, aggregation unbiased transcriptome profiling data sets minor salivary gland biopsies from controls patients identified increased expression lysosome-associated membrane protein 3...
Article24 May 2016Open Access Transparent process Phosphorylation-dependent Akt–Inversin interaction at the basal body of primary cilia Futoshi Suizu Division Cancer Biology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan Search more papers by this author Noriyuki Hirata Kohki Kimura Tatsuma Edamura Tsutomu Tanaka Satoko Ishigaki Thoria Donia Chemistry Department, Faculty Science, Tanta Tanta, Egypt Hiroko Noguchi Department Pathology, Teine Keijinkai Hospital, Toshihiko...
Lysosome-associated membrane protein 3 (LAMP3) overexpression is implicated in the development and progression of Sjögren's disease (SjD) by inducing lysosomal permeabilization (LMP) apoptotic cell death salivary gland epithelium. The aim this study was to clarify molecular details LAMP3-induced lysosome-dependent test biogenesis as a therapeutic intervention.
Abstract Endoplasmic reticulum disulfide oxidase ERO1-α plays a role in the formation of bonds collaboration with protein isomerase. Disulfide bond is required for proper conformation and function secreted cell surface proteins. We found that was overexpressed variety tumor types; therefore, we examined its growth. In BALB/c mice, knockdown within 4T1 mouse mammary gland cancer (KD) cells caused retardation vivo growth compared scrambled control (SCR) cells. contrast, when...
Endoplasmic reticulum disulfide oxidase 1-α (ERO1-α) is an that exists in the endoplasmic and has a role formation of bonds secreted proteins cell-surface proteins. Recently, we reported ERO1-α present high levels various types tumours, novel factor poor prognosis. However, how affects tumour vivo why patients who have with expression level prognosis are still unknown. Therefore, to clarify mechanism, investigated effect on from point view angiogenesis. The growth angiogenesis was analysed...
BMP6 is a central cytokine in the induction of Sjögren's syndrome–associated (SS-associated) secretory hypofunction. However, upstream initiation leading to production this SS unknown. In study, RNA ISH on salivary gland sections taken from patients with indicated monocytic lineage cells as cellular source BMP6. RNA-Seq data human glands suggested that TLR4 signaling was an regulator BMP6, which confirmed by vitro cell assays and single-cell transcriptomics PBMCs. Further investigation...
Abstract ERO1-α is an oxidizing enzyme that exists in the endoplasmic reticulum and induced under hypoxia. It reoxidizes reduced form of protein disulfide isomerase has oxidized target proteins. We found overexpressed a variety tumor types. MHC class I H chain (HC) two bonds α2 α3 domains. HC folding linked to assembly molecules because only fully disulfide-bonded HCs efficiently assemble with β2-microglobulin. In this study, we show associates isomerase, calnexin, immature before being...
// Hiroshi Saijo 1, 2 , Yoshihiko Hirohashi 1 Toshihiko Torigoe Ryota Horibe Akari Takaya Aiko Murai Terufumi Kubo Toshimitsu Kajiwara Tsutomu Tanaka Yosuke Shionoya Eri Yamamoto Reo Maruyama 3 Munehide Nakatsugawa Takayuki Kanaseki Tomohide Tsukahara Yasuaki Tamura 4 Yasushi Sasaki 5 Takashi Tokino Hiromu Suzuki Toru Kondo 6 Hiroki Takahashi Noriyuki Sato Department of Pathology, Sapporo Medical University School Medicine, Sapporo, 060-8556, Japan Respiratory Medicine and Allergology,...
Sjögren's syndrome (SS) is an autoimmune sialadenitis with unknown aetiology. Although extensive research implicated abnormal immune response associated lymphocytes, initiating event mediated by salivary gland epithelial cell (SGEC) abnormalities causing activation poorly characterised. Transcriptome studies have suggested alternations in lysosomal function are SS, but a cause and effect linkage has not been established. In this study, we demonstrated that altered lysosome activity SGECs...
Objective Lysosome‐associated membrane protein 3 (LAMP3) misexpression in salivary gland epithelial cells plays a causal role the development of dysfunction and autoimmunity associated with Sjögren's disease (SjD). This study aimed to clarify how LAMP3 is induced SjD. Methods To explore upstream signaling pathways expression, we conducted multiple RNA sequencing analyses minor glands from patients SjD, submandibular mouse model cell lines. A hypothesis generated by was further tested vitro...
Major histocompatibility complex (MHC) class I molecules play an essential role in regulating the adaptive immune system by presenting antigens to CD8 T cells. CITA (MHC transactivator), also known as NLRC5 (NLR family, CARD domain-containing 5), regulates expression of MHC and components involved antigen presentation pathway. While critical nuclear distribution its transactivation activity has been known, regulatory mechanism determine localization remains poorly understood. In this study,...
Abstract Cultivation of quinoa (Chenopodium quinoa), an annual pseudocereal crop that originated in the Andes, is spreading globally. Because highly nutritious and resistant to multiple abiotic stresses, it emerging as a valuable provide food nutrition security worldwide. However, molecular analyses have been hindered by genetic heterogeneity resulting from partial outcrossing. In this study, we generated 136 inbred lines basis for identification characterization gene functions through...
To establish an effective cancer immunotherapy, it is crucial that cells present a cancer-specific antigen in hypoxic area, hallmark of the tumor microenvironment. Here, we show impact hypoxia on MHC class I presentation vitro and vivo murine tumors. Activation antigen-specific CTLs by had been pre-incubated under condition was enhanced compared with normoxia. Cell surface expression I-peptide complex increased hypoxia, thereby leading to higher susceptibility specific CTLs. We...
Quinoa ( Chenopodium quinoa ), native to the Andean region of South America, has been recognized as a potentially important crop in terms global food and nutrition security since it can thrive harsh environments an excellent nutritional profile. Even though challenges analyzing complex heterogeneous allotetraploid genome have recently overcome, with whole genome-sequencing creation genotyped inbred lines, lack technology analyze gene function planta is major limiting factor research. Here,...
Abstract Sjögren’s disease (SjD) is a chronic autoimmune sialadenitis resulting in salivary gland hypofunction with dry mouth symptom. Previous studies showed that lysosome-associated membrane protein 3 (LAMP3) overexpression involved the development of associated SjD. However, molecular mechanisms are still unclear, and no effective treatment exists to reverse function Analysis on samples from SjD patients was decreased expression sodium–potassium-chloride cotransporter-1 (NKCC1) aquaporin...
Heterogeneity among the various molecular forms of prostate-specific antigen (PSA) has not been well characterized, despite critical importance PSA in detection prostate cancer. The purpose this study was to examine heterogeneity cancerous and noncancerous materials by extensive systematic protein analysis.A catalog established with purified from seminal fluid. This used analyze immunoblotting polyclonal antibodies.PSA showed a wider range mass, 6000 28 000 Da. did contain lower mass...
Serine-threonine kinase Akt (also known as PKB, protein B), a core intracellular mediator of cell survival, is involved in various human cancers and has been suggested to play an important role the regulation autophagy mammalian cells. Nonetheless, physiological function lysosomes currently unknown. We have reported previously that PtdIns(3)P-dependent lysosomal accumulation Akt-Phafin2 complex critical step for induction. Here, characterize molecular activated process autophagy, we searched...
Hydroxychloroquine (HCQ) is a lysosomotropic agent that commonly used for treating Sjögren’s disease (SjD). However, its efficacy controversial because of the divergent response to drug among patients. In subgroup SjD patients, lysosome-associated membrane protein 3 (LAMP3) elevated in expression salivary glands and promotes lysosomal dysregulation lysosome-dependent apoptotic cell death. this study, chloroquine (CQ) derivative HCQ were tested their ability prevent LAMP3-induced apoptosis,...