A5100693509- Gut microbiota and health
- Ginseng Biological Effects and Applications
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Pharmacological Effects of Natural Compounds
- MicroRNA in disease regulation
- Tryptophan and brain disorders
- Nanoplatforms for cancer theranostics
- Ginger and Zingiberaceae research
- Natural product bioactivities and synthesis
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- interferon and immune responses
- Inflammasome and immune disorders
- Traditional Chinese Medicine Analysis
- Retinal Diseases and Treatments
- Gastrointestinal motility and disorders
- Ferroptosis and cancer prognosis
- Nanoparticle-Based Drug Delivery
- Alkaloids: synthesis and pharmacology
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Pancreatic function and diabetes
Huashan Hospital
2012-2024
Fudan University
2012-2024
Shaoxing People's Hospital
2024
Harbin Medical University
2023-2024
Second Affiliated Hospital of Harbin Medical University
2024
Nanjing University of Chinese Medicine
2022-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2023
Zhongnan Hospital of Wuhan University
2023
Wuhan University
2023
Shanghai Changning Maternity and Infant Health Hospital
2022
Abstract Trimethylamine- N -oxide (TMAO) derived from the gut microbiota is an atherogenic metabolite. This study investigates whether or not berberine (BBR) could reduce TMAO production in and treat atherosclerosis. Effects of BBR on microbiota, as well plaque development atherosclerosis were investigated culture animal intestinal bacterial, HFD-fed animals atherosclerotic patients, respectively. We found that oral lowers biosynthesis intestine through interacting with enzyme/co-enzyme...
Morinda officinalis oligosaccharides (MOO) are an oral drug approved in China for the treatment of depression China. However, MOO is hardly absorbed so that their anti-depressant mechanism has not been elucidated. Here, we show acted on tryptophan → 5-hydroxytryptophan (5-HTP) serotonin (5-HT) metabolic pathway gut microbiota. could increase hydroxylase levels microbiota which accelerated 5-HTP production from tryptophan; meanwhile, inhibited decarboxylase activity, thus reduced 5-HT...
The aberrant expression of microRNAs (miRNAs) has been found in various types cancer. present study miR-20a was significantly up-regulated prostate cancer compared with normal tissues. Patients a higher had Gleason score 7-10 and shorter survival time. transwell wound healing assays revealed that blocking by ASO suppresses the invasion migration PC-3 DU145 cells vitro also inhibits tumor growth vivo. Furthermore, we identified directly targets ABL family non-receptor tyrosine kinases ABL2...
Paeoniflorin, the main component of Xiaoyao Wan, presents low oral bioavailability and unclear antidepressant mechanism. To elucidate potential reasons for paeoniflorin explore its mechanism from perspective gut microbiota, here, a chronic unpredictable depression model forced swimming test were firstly performed to examine effects paeoniflorin. Then pharmacokinetic study in rats was based on microbiota; meanwhile, microbiota incubated with vitro used identify possible metabolites Molecular...
Abstract Maize ( Zea mays , L.) cultivation has expanded greatly from tropical to temperate zones; however, its sensitivity chilling often results in decreased germination rates, weak seedlings with reduced survival and eventually lower yields. We conducted tests on the maize-282-diverse-panel (282 inbred lines) under normal (25 °C) (8 conditions. Three raw measurements of were recorded each condition: 1) rate, 2) days 50% germination, 3) index. relative traits derived as indicators...
Abstract Tumor cells require high levels of cholesterol for membrane biogenesis rapid proliferation during development. Beyond the acquired from low-density lipoprotein (LDL) taken up circulation, tumor can also biosynthesize cholesterol. The molecular mechanism underlying anabolism in esophageal squamous cell carcinoma (ESCC) and its effect on patient prognosis are unclear. Dysregulation lipid metabolism is common cancer. Lysophosphatidylcholine acyltransferase 1 (LPCAT1) has been...
At present, clinical interventions for chronic kidney disease are very limited, and most patients rely on dialysis to sustain their lives a long time. However, studies the gut-kidney axis have shown that gut microbiota is potentially effective target correcting or controlling disease. This study showed berberine, natural drug with low oral availability, significantly ameliorated by altering composition of inhibiting production gut-derived uremic toxins, including p-cresol. Furthermore,...
The gut microbiota plays an important role in inflammatory diseases. Metabolites the three metabolic pathways of tryptophan (Trp), histidine (His), and phenylalanine (Phe) can affect various conditions, such as obesity, diabetes, arthritis, colitis, atherosclerosis, neuroinflammation. We established LC-MS/MS method to measure 17 metabolites-Trp, 3-indole-acetic acid (Iaa), 3-indole-lactate (Ila), 3-indole-propionic (Ipa), 3-indole formaldehyde (Iald), kynurenine (Kn), kynurenic (Kyna),...
Specnuezhenide (SNZ) is among the main components of Fructus Ligustri Lucidi, which has anti-inflammation, anti-oxidation, and anti-tumor effect. The low bioavailability makes it difficult to explain mechanism pharmacological effect SNZ. In this study, role gut microbiota in metabolism pharmacokinetics characteristics SNZ as well meaning were explored. can be rapidly metabolized by microbiome, two intestinal bacterial metabolites SNZ, salidroside tyrosol, discovered. addition,...
Long non-coding RNAs (lncRNAs) have been considered as one type of gene expression regulator for cancer development, but it is not clear how these are regulated. This study aimed to identify a specific lncRNA that promotes glioma progression.RNA sequencing (RNA-seq) and quantitative real-time PCR were performed screen differentially expressed genes. CCK-8, transwell migration, invasion assays, mouse xenograft model determine the functions TMEM44-AS1. Co-IP, ChIP, Dual-luciferase reporter RNA...
Mutational activation of KRAS is a common oncogenic event in lung cancer, yet effective therapies are still lacking. Here, we identify B cell lymphoma 6 (BCL6) as lynchpin KRAS-driven cancer. BCL6 expression was increased upon tumor tissue mice and positively correlated with the KRAS-GTP, active form KRAS, various human cancer lines. Moreover, highly expressed KRAS-mutant adenocarcinomas associated poor patient survival. Mechanistically, MAPK/ERK/ELK1 signaling axis downstream mutant...
ABSTRACT Miniature pigs have advantages over rodents in modeling atherosclerosis because their cardiovascular system and physiology are similar to that of humans. Apolipoprotein E (ApoE) deficiency has long been implicated disease To establish an improved large animal model familial hypercholesterolemia atherosclerosis, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) was used disrupt ApoE gene Bama miniature pigs. Biallelic-modified...
Aims: Myocardial ischemia–reperfusion (I/R) injury facilitates cardiomyocyte death and endangers human health. N6-methyladenosine (m6A) methylation plays a critical role in cardiovascular diseases. The m6A reader YTHDF2 identifies m6A-modified RNA promotes target degradation. Hence, we hypothesized that affects I/R by regulating stability. Results: Both messenger (mRNA) protein levels of were upregulated mice hypoxia-reoxygenation (H/R)-induced cardiomyocytes. Silencing endogenous abrogated...
Poor prognosis of esophageal cancer is associated with limited clinical treatment efficacy and lack targeted therapies. With advances in nanomedicine, nanoparticle drug delivery systems play increasingly important roles tumor by enabling the simultaneous multiple therapeutic agents. We here propose a novel nanovector for combination gene therapy chemotherapy cancer. A lipid (EYLN) was designed to carry doxorubicin (Dox) small interfering RNA against anabolic metabolism LPCAT1, which we...
It has been reported that monoamine neurotransmitters can be produced by gut microbiota, and several related metabolites of amino acids in these pathways are associated with nervous system (NVS) diseases. Herein, we focused on three pathways, namely, phenylalanine (Phe), tryptophan (Trp), glutamic acid (Glu), established an underivatized liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the quantification nineteen microbiota. The included Phe, tyrosine (Tyr), l-dopa...
The bioavailability of flavonoids is generally low after oral administration. metabolic transformation by the gut microbiota may be one main reasons for this, although these metabolites have potential pharmacological activities. Liquiritigenin an important dihydroflavonoid compound found in Glycyrrhiza uralensis that has a wide range properties, such as antitumor, antiulcer, anti-inflammatory, and anti-AIDS effects, but its mechanism action remains unclear. This study explored liquiritigenin...
Plantaginis Herba (Plantago asiatica L.) has the effects of clearing heat and diuresis, oozing wet drenching. As main active components L.), plantamajoside have a wide range antitumor activities but very low bioavailability. The process interacting between gut microbiota remains unclear.To illustrate based on high-resolution mass spectrometry targeted metabolomics methods.This experiment was divided into two parts. First, metabolites produced from by were identified quantified LC-MS/MS....
Anoikis-Related Genes (ARGs) lead to the organism manifesting resistance anoikis and are associated with unfavorable prognostic outcomes across various malignancies.Therefore, it is crucial identify pivotal target genes related in HCC .We found that ARGs were significantly correlated prognosis immune responses HCC. The core gene, SPP1, notably promoted metastasis through both vivo vitro studies. PI3K-Akt-mTOR pathway played a critical role suppression within contexts. Our research unveiled...
Dietary factors play a crucial role in irritable bowel syndrome (IBS) pathogenesis. Therefore, the dietary contraindications for patients with IBS require further supplementation. Recent investigations have revealed that ginger consumption may pose risk of aggravating symptoms and incidence IBS; however, specific mechanism remains unknown. In this study, we developed experimental intestinal organoid differentiation screening models to elucidate mechanisms underlying ginger-mediated...
Glioma is one of the most common malignant tumors central nervous system, and its prognosis extremely poor. Aberrant methylation lncRNA promoter region significantly associated with glioma patients. In this study, we investigated potential impact in patients to establish a signature nine methylated genes for determining patient prognosis. Methylation data clinical follow-up were obtained from The Cancer Genome Atlas (TCGA). multistep screening strategy identified that overall survival (OS)...
Background: Chemoresistance is a major barrier to the treatment of human cancers. Circular RNAs (circRNAs) are implicated in drug resistance cancers, including gastric cancer (GC). In this study, we aimed explore functions circRNA Armadillo Repeat gene deleted Velo-Cardio-Facial syndrome (circARVCF) cisplatin (DDP) GC. Methods: The expression circARVCF, microRNA-1205 (miR-1205) and fibroblast growth factor receptor 1 (FGFR1) was detected by quantitative real-time polymerase chain reaction...