A5009635776- Gut microbiota and health
- Probiotics and Fermented Foods
- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
- Tryptophan and brain disorders
- Cancer Research and Treatments
- Metabolomics and Mass Spectrometry Studies
- Genetic and phenotypic traits in livestock
- Gastrointestinal motility and disorders
- Animal Genetics and Reproduction
- Ginseng Biological Effects and Applications
- Microbial Metabolites in Food Biotechnology
- Cancer-related molecular mechanisms research
- Tissue Engineering and Regenerative Medicine
- Genomics and Phylogenetic Studies
- Microbial Metabolism and Applications
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Virus-based gene therapy research
- Pharmacogenetics and Drug Metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Hepatocellular Carcinoma Treatment and Prognosis
- Pluripotent Stem Cells Research
- Dietary Effects on Health
Army Medical University
2015-2025
Shanghai Tenth People's Hospital
2020-2025
Tongji University
2020-2025
Army Medical College
2020-2025
Sun Yat-sen University
2010-2024
Kunming Children's Hospital
2024
The First Affiliated Hospital, Sun Yat-sen University
2010-2024
Huazhong Agricultural University
2018-2024
Union Hospital
2023-2024
Huazhong University of Science and Technology
2023-2024
The gut microbiota benefits humans via short-chain fatty acid (SCFA) production from carbohydrate fermentation, and deficiency in SCFA is associated with type 2 diabetes mellitus (T2DM). We conducted a randomized clinical study of specifically designed isoenergetic diets, together fecal shotgun metagenomics, to show that select group SCFA-producing strains was promoted by dietary fibers most other potential producers were either diminished or unchanged patients T2DM. When the fiber-promoted...
Taking the PERVs out of pigs With severe shortage organs needed for transplants, xenotransplantation (transplantation nonhuman to humans) offers an alternative source. Some pig have similar size and function those humans. The challenge is that genome harbors porcine endogenous retroviruses (PERVs) can potentially pass humans with possibly damaging consequences. Niu et al. generated in which all copies were inactivated by CRISPR-Cas9 engineering (see Perspective Denner). Not only does this...
Schizophrenia (SCZ) is a devastating mental disorder with poorly defined underlying molecular mechanisms. The gut microbiome can modulate brain function and behaviors through the microbiota-gut-brain axis. Here, we found that unmedicated medicated patients SCZ had decreased α-diversity index marked disturbances of microbial composition versus healthy controls (HCs). Several unique bacterial taxa (e.g., Veillonellaceae Lachnospiraceae) were associated severity. A specific panel...
Background & AimsAltered gut microbiota is implicated in development of colorectal cancer (CRC). Some intestinal bacteria have been reported to potentiate carcinogenesis by producing genotoxins, altering the immune response and microenvironment, activating oncogenic signaling pathways. We investigated whether stool from patients with CRC could directly induce mice.MethodsWe obtained stored samples participants a metagenome study performed Hong Kong. Conventional (male C57BL/6) mice were...
Antioxidant and antipromotional effects of the soybean isoflavone genistein have been studied in HL-60 cells mouse skin tumorigenesis model. Effects structure-related flavone/isoflavones on hydrogen peroxide (H2O2) production by 12-O-tetradecanoylphorbol-13-acetate (TPA)-activated superoxide anion (O2-) generation xanthine/xanthine oxidase were compared. Of tested isoflavones, is most potent inhibitor among TPA-induced H2O2 formation (dimethyl sulfoxide) DMSO-differentiated cells, daidzein...
Gut microbiota has been implicated as a pivotal contributing factor in diet-related obesity; however, its role development of disease phenotypes human genetic obesity such Prader–Willi syndrome (PWS) remains elusive. In this hospitalized intervention trial with PWS (n = 17) and simple 21) children, diet rich non-digestible carbohydrates induced significant weight loss concomitant structural changes the gut together reduction serum antigen load alleviation inflammation. Co-abundance network...
Objective Programmed death 1 and its ligand (PD-1/PD-L1) immunotherapy is promising for late-stage lung cancer treatment, however, the response rate needs to be improved. Gut microbiota plays a crucial role in sensitisation Panax ginseng has been shown possess immunomodulatory potential. In this study, we aimed investigate whether combination treatment of polysaccharides (GPs) αPD-1 monoclonal antibody (mAb) could sensitise by modulating gut microbiota. Design Syngeneic mouse models were...
Abstract Gut microbiota has an important role in the immune system, metabolism, and digestion, a significant effect on nervous system. Recent studies have revealed that abnormal gut induces behaviors, which may be associated with hypothalamic–pituitary–adrenal (HPA) axis. Therefore, we investigated behavioral changes germ-free (GF) mice by tests, quantified basal serum cortisol levels, examined glucocorticoid receptor pathway genes hippocampus using microarray analysis followed real-time PCR...
Abstract Genetics alone cannot explain most cases of rheumatoid arthritis (RA). Thus, investigating environmental factors such as the gut microbiota may provide new insights into initiation and progression RA. In this study, we performed 16S rRNA sequencing to characterise DBA1 mice that did or not develop after induction with collagen. We found divergence in distribution was pronounced significant. Mice susceptible collagen-induced (CIA) showed enriched operational taxonomic units (OTUs)...
Objective Cigarette smoking is a major risk factor for colorectal cancer (CRC). We aimed to investigate whether cigarette smoke promotes CRC by altering the gut microbiota and related metabolites. Design Azoxymethane-treated C57BL/6 mice were exposed or clean air 2 hours per day 28 weeks. Shotgun metagenomic sequencing liquid chromatography mass spectrometry parallelly performed on stools alterations in Germ-free transplanted with from smoke-exposed smoke-free control mice. Results Mice had...
Intestinal microbes are an important system in the human body, with significant effects on behavior. An increasing body of research indicates that intestinal affect brain function and neurogenesis, including sensitivity to stress. To investigate microbial colonization behavior, we examined behavioral changes associated hormones hormone receptors hypothalamic-pituitary-adrenal (HPA) axis under We tested germ-free (GF) mice specific pathogen-free (SPF) mice, divided into four groups. A chronic...
Inflammasomes are involved in gut homeostasis and inflammatory pathologies, but the role of NLRP3 inflammasome these processes is not well understood. Cryopyrin-associated periodic syndrome (CAPS) patients with mutations have autoinflammation skin, joints, eyes, intestine. Here we show that intestines CAPS model mice carrying an Nlrp3 R258W mutation maintain gut. Additionally, such strongly resistant to experimental colitis colorectal cancer; this mainly through a remodelled microbiota...
Abstract Large-scale fecal shotgun metagenomic sequencing revealed the high abundance of Parvimonas micra in colorectal cancer (CRC) patients. We investigated role and clinical significance P. tumorigenesis. The was examined 309 samples 165 colon biopsy tissues CRC patients healthy subjects. significantly enriched from 128 compared to 181 subjects ( P < 0.0001); tissue biopsies 52 61 0.0001). Multivariate analysis showed that is an independent risk factor poor survival (Hazard Ratio:...
Characteristics of the gut microbiome vary synchronously with changes in host diet. However, underlying effects these fluctuations remain unclear. Here, we performed fecal microbiota transplantation (FMT) diet-specific feces from an endangered mammal (the giant panda) into a germ-free mouse model. We demonstrated that butyrate-producing bacterium Clostridium butyricum was more abundant during shoot-eating season than leaf-eating season, congruent significant increase body mass. Following...
Diarrheic disease is a common intestinal health problem worldwide, causing great suffering to humans and animals. Precise manipulation strategies based on probiotics combat diarrheic diseases have not been fully developed.
Abstract Melatonin influences intestinal microbiota and the pathogenesis of various diseases. This study was conducted to explore whether melatonin alleviates weanling stress through in a mouse model. supplementation mice (provided drinking water at dosage 0.2 mg/mL for 2 weeks) significantly improved body weight gain (1.4 ± 0.03 g/day group vs 1.2 0.06 control group) morphology (ie, villus length, crypt depth, ratio), but had little effect on proliferation or apoptosis cells, numbers Paneth...
NOD2 functions as an intracellular sensor for microbial pathogen and plays important role in epithelial defense. The loss-of-function mutation of is strongly associated with human Crohn’s disease (CD). However, the mechanisms how maintains intestinal homeostasis regulates susceptibility CD are still unclear. Here we found that numbers intraepithelial lymphocytes (IELs) were reduced significantly Nod2−/− mice residual IELs displayed proliferation increased apoptosis. Further study showed...
Alterations in the intestinal microbiota affect development of colorectal cancer and drug metabolism. We studied whether ability aspirin to reduce colon tumor mice.We performed studies with APCmin/+ mice given azoxymethane dextran sulfate sodium induce carcinogenesis. Some were antibiotics deplete microbes, or without aspirin, throughout entire experiment. Germ-free validation experiments. Colon tissues collected analyzed by histopathology, quantitative reverse-transcription polymerase chain...