A5044265499- Gut microbiota and health
- Ginseng Biological Effects and Applications
- Protein Kinase Regulation and GTPase Signaling
- Fungal Biology and Applications
- Natural product bioactivities and synthesis
- Wnt/β-catenin signaling in development and cancer
- Probiotics and Fermented Foods
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Polysaccharides and Plant Cell Walls
- PI3K/AKT/mTOR signaling in cancer
- Phytochemicals and Antioxidant Activities
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Phytochemistry and Biological Activities
- Plant Gene Expression Analysis
- Cancer Mechanisms and Therapy
- Food Allergy and Anaphylaxis Research
- Enzyme function and inhibition
- Medicinal Plant Pharmacodynamics Research
- Pediatric health and respiratory diseases
- Chromosomal and Genetic Variations
Foshan Maternity and Child Health Care Hospital
2022-2024
Southern Medical University
2022-2024
Macau University of Science and Technology
2015-2024
Genomics Research Center, Academia Sinica
2022
Quality Research
2021
University of Macau
2018
Hong Kong Baptist University
2004-2012
Chinese Medical Center
2005-2010
University of Hong Kong
2000-2004
Hong Kong University of Science and Technology
2000-2004
Objective Programmed death 1 and its ligand (PD-1/PD-L1) immunotherapy is promising for late-stage lung cancer treatment, however, the response rate needs to be improved. Gut microbiota plays a crucial role in sensitisation Panax ginseng has been shown possess immunomodulatory potential. In this study, we aimed investigate whether combination treatment of polysaccharides (GPs) αPD-1 monoclonal antibody (mAb) could sensitise by modulating gut microbiota. Design Syngeneic mouse models were...
Purpose: To develop an in situ gel system comprising liposome-containing paclitaxel (PTX) dispersed within the thermoreversible (Pluronic ® F127 gel) for controlled release and improved antitumor drug efficiency. Methods: The dialysis membrane membrane-less diffusion method were used to investigate vitro behavior. Differential scanning calorimetry (DSC) thermal analysis was "micellization" "sol/gel transition" process of systems. In cytotoxicity uptake KB cancer cells determined by MTT,...
Abstract Studies showed that manipulation of gut microbiota (GM) composition through the treatment prebiotics could be a novel preventive measure against colorectal cancer (CRC) development. In this study, for first time, we assessed non-toxic doses triterpene saponins (ginsenoside-Rb3 and ginsenoside-Rd) – as effectively reinstated dysbiotic-gut microbial intestinal microenvironment in an Apc Min/+ mice model. Rb3 Rd reduced size number polyps accompanied with downregulation oncogenic...
Abstract Frizzled‐related protein (Frp) is a new family of secreted proteins that contain region homologous to the extracellular cysteine‐rich domain (CRD) frizzled proteins. The role Frp far from clear. To explore and its relationship Wnt‐signalling pathway in breast cancer, situ hybridization immunohistochemical analyses Frp, Wnt‐1, APC, β‐catenin, target genes c‐ myc cyclin D1 were conducted 70 specimens invasive ductal carcinomas human breast. mRNA was down‐regulated 62 elevated eight...
In the present study, we investigated prognostic and diagnostic significance of beta-catenin nuclear immunostaining in 60 specimens normal colorectal tissue; 180 polyps, adenomas, carcinomas; 40 from patients with simultaneous occurrence carcinomas. Additional 59 carcinoma 14 adenoma who subsequently developed were examined for possible survival study. Immunohistochemical staining showed that correlated sequential stages carcinogenesis, which positive was observed 0% tissues, 8% 92% 100%...
Carboxyl-containing metabolites (CCMs) widely exist in living systems and are the essential components for life. Global characteristics of CCMs biological samples critical understanding physiological processes discovery onset relevant diseases. However, their determination represents a challenge due to enormous polarity differences, structural diversity, high similarity, poor ionization efficiency mass spectrometry. Herein, 5-(diisopropylamino)amylamine (DIAAA) derivatization coupled with...
Saponins derived from medicinal plants have raised considerable interest for their preventive roles in various diseases. Here, we investigated the impacts of triterpenoid saponins isolated Gynostemma pentaphyllum (GpS) on gut microbiome, mucosal environment, and effect tumor growth. Six-week old ApcMin/+ mice wild-type littermates were fed either with vehicle or GpS daily duration 8 weeks. The fecal microbiome was analyzed by enterobacterial repetitive intergenic consensus (ERIC)-PCR 16S...
In order to understand the intricate relationship of cell proliferation and apoptosis in tumour development, markers (Ki-67 c-myc), apoptosis, cell-cycle inducers cyclin D1 D3, inhibitors p16INK4, p21CIP1, p27KIP1 were evaluated ductal breast carcinoma. The heterogeneous nature tumours provides a system by which changes genes can be explored under wide range apoptotic indices. To address above issues, immunohistochemical studies conducted 40 pairs adjacent normal tissues. TUNEL method was...
We recently developed rat fibroblast cell lines that stably overproduce high levels of the beta 1 form protein kinase C (PKC). These cells display several disorders in growth control and small microscopic colonies agar. In present study we demonstrate one these lines, R6-PKC3, is extremely susceptible to transformation by an activated human bladder cancer c-H-ras oncogene (T24). Compared with line R6-C1, T24-transfected R6-PKC3 yielded a 10-fold increase formation large Cell established from...
Pogostemon cablin (Blanco) Benth (PC) is a Chinese medicinal plant traditionally used for the treatment of gastrointestinal symptoms. To investigate prebiotic effect patchouli essential oil (PEO) and its derived compounds through modulation gut microbiota (GM). C57BL/6J mice were treated with PEO three active components PEO, i.e. alcohol (PA), pogostone (PO) β-patchoulene (β-PAE) 15 consecutive days. Fecal samples mucosa collected GM biomarkers studies. PA, PO, β-PAE improve epithelial...
Colorectal cancer (CRC) ranks the third most common type in both men and women. Besides known genetic epigenetic changes gut epithelial cells, we now know that disturbed microbes could also contribute to onset progression of CRC. Hence, keeping a balanced microbiota (GM) has become novel pursue medical field, particularly area gastrointestinal disorders. Gynostemma pentaphyllum (Gp) is dietary herbal medicine. In our previous study, Gp saponins (GpS) displayed prebiotic cancer-preventive...
Abstract Background As a first-line chemotherapeutic agent, 5-fluorouracil (5-FU) exhibits many side effects, weakening its efficacy in cancer treatment. In this study, we hypothesize that Poria cocos polysaccharides (PCP), traditional Chinese herbal medicine with various bioactivities and prebiotic might improve the therapeutic effect of 5-FU by restoring homeostasis gut microenvironment commensal microflora. Methods Apc Min/ + mice were employed to evaluate anti-cancer conjunction PCP Body...
In the accompanying study (Borner, C.B., Guadagno, S. N., and Weinstein, I. B. (1992) J. Biol. Chem. 267, 12892-12899) we found that R6 embryo fibroblasts express four isoforms of PKC, cPKC alpha, nPKC epsilon, delta, zeta whose subcellular distribution, activation, down-regulation are differentially regulated. Furthermore, demonstrated overproduction an exogenous beta I isoform in these cells (R6-PKC3) altered TPA-induced delta epsilon. this paper show transformation or R6-PKC3 with a...