A5001794632- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Renal and related cancers
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- Genetic and phenotypic traits in livestock
- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
- Genetic Syndromes and Imprinting
- Microbial Inactivation Methods
- Congenital heart defects research
- Veterinary Equine Medical Research
- Xenotransplantation and immune response
- Reproductive System and Pregnancy
- Virus-based gene therapy research
- Kruppel-like factors research
- Plant Genetic and Mutation Studies
- Effects of Environmental Stressors on Livestock
Inner Mongolia Agricultural University
2017-2025
Ministry of Agriculture and Rural Affairs
2023-2025
Northeast Agricultural University
2009-2022
Transgenic animals have been used for years to study gene function, produce important proteins, and generate models the of human diseases. However, inheritance expression instability transgene in transgenic is a major limitation. Copy number promoter methylation are known regulate expression, but no report has systematically examined their effect on expression. In study, we generated two pigs by somatic cell nuclear transfer (SCNT) that express green fluorescent protein (GFP) driven...
Background : Mammalian first lineage segregation generates trophectoderm (TE) and pluripotent inner cell mass (ICM), which provides an ideal model for studying the mechanisms of maintenance loss pluripotency. In mouse, transcription factor OCT4 restricts to ICM plays a key role in TE/ICM specification regulatory networks. However, pig, does not restrict cells, suggesting different molecular basis Results To explore porcine networks, we examined expression pattern pluripotency factors,...
The role of CDX2 in trophectoderm (TE) cells has been extensively studied, yet the results are contradictory and species specific. Here, expression function were explored early porcine embryos. Notably, siRNA-mediated gene knockdown lentivirus-mediated TE-specific regulation demonstrated that is essential for maintenance blastocyst integrity by regulating BMP4-mediated niche classic protein kinase C (PKC)-mediated TE polarity mammalian Mechanistically, CDX2-depleted embryos stalled at stage...
Although the pig is considered an important model of human disease and ideal animal for preclinical testing cell transplantation, utility this has been hampered by a lack genuine porcine embryonic stem cells. Here, we derived pluripotent (pPSC) line from day 5.5 blastocysts in newly developed culture system based on MXV medium 5% oxygen atmosphere. The pPSCs had passaged more than 75 times over two years, morphology colony was similar to that Characterization assessment showed were alkaline...
Abstract. Cryopreservation of semen has advanced significantly with the development artificial insemination techniques, but post-thawed sperm often exhibit reduced viability, membrane integrity, and acrosome integrity compared to fresh sperm, leading decreased fertilization capacity. Oxidative stress is a major concern during cryopreservation. This study investigated use resveratrol (RSV), potent antioxidant, in cryopreservation Mongolian horse semen. Different concentrations RSV were...
Background/Objectives: The success or failure of embryo fixation is crucial for attachment and later development. As an epithelial chorioallantoic placenta-type animal, the horse has a special process implantation, mechanism in horses still unclear. Methods: In this study, structural transcriptomic characteristics endometrial tissue from fixed nonfixed sides 20-day gestation embryos Mongolian were investigated to search important genes potential molecular markers associated with phase equine...
Introduction: Equine chorionic girdle cells can give rise to the endometrial cup, a structure that secretes equine gonadotropin (eCG). To date, mechanisms underlying proliferation, differentiation, invasion, and hormone secretion of are not fully understood. During human pregnancy, interleukin-6 (IL-6) is maternally expressed stimulate invasion migration syncytiotrophoblast influence synthesis gonadotropin. Although many previous studies reported same upregulation IL-6 during placentation,...
The low success rate of somatic cell nuclear transfer (SCNT) in mammalian cloning is largely due to imprinting problems. However, little known about the mechanisms reprogramming imprinted genes during SCNT. Parental origin-specific DNA methylation regulates monoallelic expression genes. In natural fertilization, imprints are established parental germline and maintained throughout embryonic development. it unclear whether protected from global changes cloned preimplantation embryos. Here, we...
In response to muscle injury, stem cells are stimulated by environmental signals integrate into damaged tissue mediate regeneration. L-leucine (L-leu), a branched-chain amino acid (BCAA) that belongs the essential acids (AAs) of animal, has gained global interest on account its muscle-building and regenerating effects. The present study was designed investigate impact L-leu exposure promote proliferation equine skeletal satellite (SCs) regulation RNA networks, including mRNA, long non-coding...
Previous research has shown that leucine (Leu) can stimulate and enhance the proliferation of equine skeletal muscle satellite cells (SCs). The gene expression profile associated with Leu-induced SCs also been documented. However, specific role Leu in regulating slow-twitch fibers (slow-MyHC) mitochondrial function SCs, as well underlying mechanism, remains unclear. During this investigation, underwent culturing differentiation medium were subjected to varying concentrations (0 mM, 0.5 1 2 5...
Testicular development and spermatogenesis are tightly regulated by both coding non-coding genes, with mRNA lncRNA playing crucial roles in post-transcriptional gene expression regulation. However, there significant differences regulatory mechanisms before after sexual maturity. Nevertheless, the mRNAs lncRNAs testes of Mongolian horses have not been systematically identified. In this study, we first identified testicular tissues sexually immature mature at tissue protein levels,...
The well known and most important function of nucleoli is ribosome biogenesis. However, the nucleolus showed delayed development malfunction in somatic cell nuclear transfer (NT) embryos. Previous studies indicated that nearly half rRNA genes (rDNA) cells were inactive not transcribed. We compared rDNA methylation level, active nucleolar organizer region (NORs) numbers, proteins (upstream binding factor (UBF), nucleophosmin (B23)) distribution, nucleolar-related gene expression three...
Abstract Nanog as an important transcription factor plays a pivotal role in maintaining pluripotency and reprogramming the epigenome of somatic cells. Its ability to function on committed cells embryos has been well defined mouse human, but rarely pig. To better understand Nanog's porcine fetal fibroblast (PFF) nuclear transfer (NT) embryo, we cloned CDS constructed pcDNA3.1 (+)/Nanog pEGFP‐C1/Nanog overexpression vectors transfected them into PFFs. We studied cell biological changes...
Oct4 is exclusively expressed in rodent inner cell mass (ICM) but silenced its trophectoderm (TE). However, for many non-rodent animals, including pig, cattle, rabbit, goat, and human, OCT4 has a remarkable expression early TE. This study, applying pig as the main research model, proves that TE supported by unique GATA motif upstream conserved regulatory region, GATA4 responsible activation. Moreover, acts specific regulator of narrow range genes (including BCL2A1 HNRNP2AB1) are essential...
The proliferation and differentiation of skeletal muscle satellite cells (SCs) are necessary for the development mature muscle. Leucine (Leu) is both an essential amino acid (EAA) a branched-chain (BCAA), which has attracted worldwide attention due to its ability repair become new fibers. We separated equine SCs into control group (CON) Leu-supplemented (LEU), were cultured in Leu-deprived media respectively. combined transcriptome (RNA-Seq) quantitative proteome (TMT) profiling analyses on...
The reproductive performance of horse sperm and donkey has been reported to differ. Sperm proteins play a crucial role in viability fertility. Although differences between species are known, no prior study investigated disparities the proteome horses donkeys. Therefore, this characterized compared proteomes donkeys using 4D-DIA mass spectrometry technology. We identified 3436 3404 sperm. Of these, 3363 were expressed both sperm, with 73 being specifically 41 According data analysis,...
During follicular development, changes in the composition of fluid are synchronized with development oocytes. Our aim was to screen key factors affecting oocyte maturation and optimize vitro culture protocol by understanding proteins metabolites fluid. Follicles divided into three groups according their diameter (small follicle (SFF): 10 mm < d 20 mm; medium (MFF): 30 large (LFF): d). Proteins from were analyzed mass spectrometry. The results showed that: LFF vs MFF, differential abundant...
Myostatin (MSTN), a member of the transforming growth factor-β superfamily, inhibits activation muscle satellite cells. However, role and regulatory network MSTN in equine cells are not well understood yet. We discovered that knockdown significantly reduces proliferation rate In addition, after RNA sequencing transfected with MSTN-interference plasmid control plasmid, an analysis differentially expressed genes was carried out. It revealed networks mainly involve related to function...
Upregulation of Cdx2 expression in outer cells is a key event responsible for cell lineage segregation between the inner mass and trophoderm (TE) mouse morula‐stage embryos. In TE cells, polarization can regulate Hippo Rho‐associated kinase (Rho‐ROCK) signaling to induce nuclear location YAP, which has been demonstrated further . However, we found that CDX2 could not be detected porcine embryos but only some at early blastocyst stage. The biological significance regulation mechanism this...