A5101825698- Reproductive Biology and Fertility
- Assisted Reproductive Technology and Twin Pregnancy
- Prenatal Screening and Diagnostics
- Ovarian function and disorders
- Reproductive Health and Technologies
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Inflammatory mediators and NSAID effects
- Amyotrophic Lateral Sclerosis Research
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Esophageal Cancer Research and Treatment
- Birth, Development, and Health
- Renal and related cancers
- Organ and Tissue Transplantation Research
- Reproductive System and Pregnancy
- Advanced biosensing and bioanalysis techniques
- Plant Virus Research Studies
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Reproductive tract infections research
- Ectopic Pregnancy Diagnosis and Management
- Tissue Engineering and Regenerative Medicine
- Gastric Cancer Management and Outcomes
- Adipose Tissue and Metabolism
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2016-2024
Wenzhou Medical University
2015-2024
Hunan Provincial Maternal and Child Health Hospital
2024
Shanghai Institute of Planned Parenthood Research
2019
Shanxi Agricultural University
2017
University of Cologne
2005-2007
Beijing Hospital
2007
Klinik und Poliklinik für Psychosomatik und Psychotherapie
2005
Cpf1 nucleases were recently reported to be highly specific and programmable with efficiencies comparable those of SpCas9. AsCpf1 LbCpf1 require a single crRNA recognize 5'-TTTN-3' protospacer adjacent motif (PAM) at the 5' end for genome editing. For widespread application in precision site-specific human editing, range sequences that can is limited due size this PAM. To address limitation, we sought identify novel nuclease simpler PAM requirements. Specifically, here test engineer FnCpf1,...
This study was designed to clarify whether transplantation of fetal olfactory ensheathing cells (OECs) would affect the clinical course patients with amyotrophic lateral sclerosis (ALS). Thirty-five probable or definite ALS were enrolled from December 2004 September 2006; 15 received OECs and 20 did not receive transplantation. cultured injected into bilateral corona radiata involving pyramidal tracts frontal lobes. The primary end point used indicate effectiveness rate change according...
Adenine base editors (ABEs) are novel genome-editing tools, and their activity has been greatly enhanced by eight additional mutations, thus named ABE8e. However, elevated catalytic was concomitant with frequent generation of bystander mutations. This effect precludes its safe applications required in human gene therapy. To develop next-generation ABEs that both catalytically efficient positionally precise, we performed combinatorial engineering NG-ABE8e. We identify a variant (NG-ABE9e),...
Assisted reproductive technology (ART) is the most effective method to treat infertility and pathogenesis of implantation failure after in vitro fertilization-embryo transfer (IVF-ET) a challenging filed infertility. Microbes female tract are considered be associated with gynecological obstetric diseases. However, its effects on embryo unsured.
Testosterone deficiency is associated with increased serum cholesterol levels. However, how testosterone precisely affects metabolism remains unclear. Therefore, in the current study, we examined effect of on and liver gene expression pigs fed a high-fat high-cholesterol (HFC) diet. Sexually mature male miniature (6–7 months old) were randomly divided into 3 groups as follows: intact an HFC diet (IM + HFC), castrated (CM replacement T). Serum levels lipid profiles measured, hepatic...
Background Kawasaki disease (KD) is a self-limiting and acute systemic vasculitis of unknown etiology, mainly affecting children. Ferulic acid (FA), natural phenolic substance, has multiple pharmacological properties, including anti-inflammatory, anti-apoptosis, anti-fibrosis, so on. So far, the protective effects FA on KD have not been explored. Methods In this study, we established Candida albicans water soluble fraction (CAWS)-induced mouse coronary artery model tumor necrosis factor α...
Abstract Leydig cells (LCs) play crucial roles in producing testosterone, which is critical the regulation of male reproduction and development. Low levels testosterone will lead to hypogonadism. LC transplantation a promising alternative therapy for However, source LCs limits this strategy clinical applications. Thus far, others have reported that can be derived from stem by gene transfection, but safe effective induction method has not yet been reported. Here, we report Leydig-like human...
Clustered regularly interspaced short palindromic repeats and associated proteins (CRISPR-Cas) of bacterial adaptive immunity have been adopted as a powerful versatile tool for manipulation the genome. This paradigm has widely applied in biological research treatments animal or cellular disease models. A critical feature CRISPR-Cas is protospacer adjacent motif (PAM), which dictates DNA target recognition mechanism Cas proteins. While, direct identifying functional PAM sequences human cells...
Abstract Decellularization processes may variably distort or degrade extracellular matrix (ECM) structure. In this study, two perfusion routes (PR) were tested on SD rat lung samples. One decellularization protocol, PR1, was perfused through the pulmonary artery. The other PR2, trachea. Both protocols used by same detergent‐based (sodium dodecyl sulphate and Triton X‐100) with different flow continuous perfusion. There no visible difference in vessel architecture between PR1‐...
UCP2 plays a physiological role by regulating mitochondrial biogenesis, maintaining energy balance, ROS elimination, and cellular autophagy in numerous tissues. But the exact roles of cumulus cells are still not clear. Genipin, special inhibitor, was added into cultural medium to explore human cells. There were no significant differences ATP membrane potential levels from inhibiting groups as compared with control. The Mn-SOD markedly elevated after inhibited Genipin. However, ratio reduced...
Oogenesis is a lengthy, multi-step process occurring in mammals yielding single or multiple oocytes capable of being fertilized upon interaction with male gametes. The overall highly complex nature, starting the primordial follicles, and its ultimate completion preceded by meiotic cycle. There are two major phases oogenesis: growth phase, followed maturation phase that requires relatively less time. Both require energy for various metabolic processes oocytes. requirements timing vary...
This study was designed to assess perinatal and neonatal outcomes of dizygotic twin pregnancies conceived naturally or by in vitro fertilization (IVF). After strict selection, the included 470 pregnancies. There were 249 resulting from IVF treatments 221 conceiving spontaneously. adjusting maternal age primiparity, results showed that there no significant differences between two groups ( P > 0.05) terms antenatal complications outcomes. In conclusion, our does not reveal increased risks...
Contents The aim of this study was to determine whether the effect Bax and Bcl‐2 on apoptosis germ cells is caused by local testicular heating (42°C, 1 hr) in boar testis. testes three boars were exposed 42°C for hr. Three other assigned as control (no heat treatment). After 6 hr treatment, all castrated harvested. Immunohistochemical results showed that a redistribution stress, expressed cytoplasm nucleus. Western blot analyses quantitative real‐time polymerase chain reaction ( QRT ‐ PCR )...
Pulmonary fibrosis is a respiratory disease caused by the proliferation of fibroblasts and accumulation extracellular matrix (ECM). It known that lung ECM mainly composed three-dimensional fiber mesh filled with various high-molecular-weight proteins. However, small-molecular-weight proteins in their differences between normal fibrotic are largely unknown.Healthy adult male Sprague-Dawley rats (Rattus norvegicus) weighing about 150 to 200 g were randomly divided into three groups using...
This retrospective cohort study explores whether noninvasive chromosome screening (NICS) for aneuploidy can improve the clinical outcomes of patients with recurrent pregnancy loss (RPL) or repeated implantation failure (RIF) in assisted reproductive technology. A total 273 women a history RPL RIF between 2018 and 2021 were included this study. We collected data all oocyte retrieval cycles single blastocyst resuscitation transfer cycles. For experiencing RPL, NICS reduced miscarriages rate...
Learning points for cliniciansTo date, only three GREB1L mutation cases linked to renal agenesis (RA) have been reported in Asia.In this study, we found the first Asian families of bilateral RA with deafness and unilateral uterine abnormalities associated heterozygous mutations (NM_001142966.2:c.3635del: p. Pro1212Hisfs à 21and c.5647A>C: Ser1883Arg), respectively.The findings will expand spectrum increase accuracy prenatal counseling future pregnancies.
Aims: Risk reduction for Barrett's cancer in individuals taking non‐steroidal anti‐inflammatory drugs has been reported. Cyclooxygenase (COX)‐2, one of the inhibited enzymes, is putatively involved pathogenesis. The aim this study was to examine a possible association between COX‐2 protein expression and development progression metaplasia–dysplasia–carcinoma sequence type degree associated inflammatory reaction. Methods results: Squamous epithelium, metaplastic, low‐grade, high‐grade...
Clustered interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome engineering technologies are sparking a new revolution in biological research. This technology efficiently induces DNA double strand breaks at the targeted genomic sequence and results indel mutations by error-prone process of nonhomologous end joining repair or homologous recombination with template. The efficiency editing CRISPR/Cas9 alone human embryonic stem cells is still low....