A5100443820- Signaling Pathways in Disease
- Genomics and Chromatin Dynamics
- Histone Deacetylase Inhibitors Research
- Amyotrophic Lateral Sclerosis Research
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Ion channel regulation and function
- Traumatic Brain Injury and Neurovascular Disturbances
- Nicotinic Acetylcholine Receptors Study
- Cytokine Signaling Pathways and Interactions
- Protein Degradation and Inhibitors
- Parkinson's Disease Mechanisms and Treatments
- Peripheral Neuropathies and Disorders
- Cancer-related Molecular Pathways
- Myasthenia Gravis and Thymoma
- Ubiquitin and proteasome pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenetic and Muscular Disorders Research
- RNA modifications and cancer
- DNA Repair Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Genetics, Bioinformatics, and Biomedical Research
- Peptidase Inhibition and Analysis
National Institute of Biological Sciences, Beijing
2022-2025
University of Southern California
2015-2024
Chinese Academy of Sciences
2013-2024
Nanjing University of Chinese Medicine
2020-2024
Shanghai Institute of Materia Medica
2019-2024
Second Hospital of Hebei Medical University
2022-2024
Fudan University
2009-2024
Hebei Medical University
2022-2024
Nanjing Drum Tower Hospital
2024
Huashan Hospital
2009-2024
Genes are not simply turned on or off, but instead their expression is fine-tuned to meet the needs of a cell. How genes modulated so precisely well understood. The glucocorticoid receptor (GR) regulates target by associating with specific DNA binding sites, sequences which differ between genes. Traditionally, these sites have been viewed only as docking sites. Using structural, biochemical, and cell-based assays, we show that GR sequences, differing little single base pair, differentially...
Significance We provide a method for population-based structure modeling of whole diploid genomes using Hi-C data. The considers the stochastic nature chromosome structures, which allows detailed analysis dynamic landscape genome organizations. predict and experimentally validate presence chromosome-specific higher-order centromere clusters, can play key role in spatial organization human genome, specifically influencing overall positioning, as well preference specific conformations. Our...
Sleep need accumulates during waking and dissipates sleep to maintain homeostasis (process S). Besides the regulation of daily (baseline) amount, homeostatic commonly refers universal phenomenon that deprivation (SD) causes an increase need, hence, amount intensity subsequent recovery sleep. The central regulators signaling pathways govern baseline regulations in mammals remain unclear. Here, we report enhanced activity calcineurin Aα (CNAα)—a catalytic subunit calcineurin—in mouse brain...
CBF1 is a member of the CSL family DNA binding factors, which mediate either transcriptional repression or activation. proteins play central role in Notch signaling and Epstein–Barr virus-induced immortalization. transmembrane protein involved cell-fate decisions, cytoplasmic domain (NotchIC) targets CBF1. The virus-immortalizing EBNA2 activates both cellular viral gene expression by targeting mimicking NotchIC. We have examined mechanism CBF1-mediated show that binds to unique corepressor,...
Two-component systems (TCSs) are important for the adaptation and survival of bacteria fungi under stress conditions. A TCS is often composed a membrane-bound sensor histidine kinase (SK) response regulator (RR), which relayed through sequential phosphorylation steps. However, mechanism how an SK switched on in to environmental stimuli remains obscure. Here, we report crystal structure complete cytoplasmic portion SK, VicK from Streptococcus mutans. The overall long-rod dimer that anchors...
Abstract Allergic asthma is a complex and chronic inflammatory disorder that associated with airway hyperreactivity (AHR) driven by Th2 cytokine secretion. Type 2 innate lymphoid cells (ILC2s) produce large amounts of cytokines contribute to the development AHR. Here, we show ILC2s express α7-nicotinic acetylcholine receptor (α7nAChR), which thought have an anti-inflammatory role in several diseases. We engagement specific agonist α7nAChR on reduces ILC2 effector function represses...
Abstract The tumor suppressor p53 is mutated in approximately half of all human cancers. can induce apoptosis through mitochondrial membrane permeabilization by interacting with and antagonizing the anti-apoptotic proteins BCL-xL BCL-2. However, mechanisms which induces remain elusive. Here, we report a 2.5 Å crystal structure p53/BCL-xL complex. In this structure, two molecules interact as homodimer, bind one molecule to form ternary complex 2:1 stoichiometry. Mutations at dimer interface...
GATA transcription factors regulate during development and differentiation by recognizing distinct sites with a tandem of two conserved zinc fingers, mediating long-range DNA looping. However, the molecular basis these processes is not well understood. Here, we determined three crystal structures full DNA-binding domain (DBD) human GATA3 protein, which contains both in complex different sites. In one structure, fingers wrap around palindromic site, cooperatively enhancing binding affinity...
The p53 core domain binds to response elements (REs) that contain two continuous half-sites as a cooperative tetramer, but how recognizes discontinuous REs is not well understood. Here we describe the crystal structure of bound naturally occurring RE located at promoter Bcl-2-associated X protein (BAX) gene, which contains one base-pair insertion between half-sites. Surprisingly, forms tetramer on BAX-RE nearly identical what has been reported other with 0-bp spacer. Each dimer in register...
Plasmodium falciparum causes the most severe form of malaria in humans and is responsible for over 700,000 deaths annually. It an obligate intracellular parasite invades erythrocytes where it grows a relatively protected niche. Invasion essential survival this involves interplay multiple protein–protein interactions. One important interactions binding invasion ligand families EBLs PfRhs to host receptors on surface erythrocytes. PfRh5 only within PfRh family vaccine candidate. binds receptor...
<h3>Objective</h3> To determine whether lipids and apolipoproteins predict prognosis of patients with amyotrophic lateral sclerosis in a cohort study 99 who were diagnosed during 2015 to 2018 followed up until October 31, 2018, at the Neurology Clinic Karolinska University Hospital Stockholm, Sweden. <h3>Methods</h3> Total cholesterol, low-density lipoprotein high-density triglycerides, apolipoprotein AI, B, lipid ratios measured time diagnosis or shortly thereafter. Death after was used as...
Abstract Accurate transcription is required for the faithful expression of genetic information. However, relatively little known about molecular mechanisms that control fidelity transcription, or conservation these across tree life. To address issues, we measured error rate in five organisms increasing complexity and found RNA polymerase II ranges from 2.9 × 10 −6 ± 1.9 −7 /bp yeast to 4.0 5.2 worms, 5.69 8.2 flies, 4.9 3.6 mouse cells 4.7 9.9 −8 human cells. These rates were modified by...
This study was designed to clarify whether transplantation of fetal olfactory ensheathing cells (OECs) would affect the clinical course patients with amyotrophic lateral sclerosis (ALS). Thirty-five probable or definite ALS were enrolled from December 2004 September 2006; 15 received OECs and 20 did not receive transplantation. cultured injected into bilateral corona radiata involving pyramidal tracts frontal lobes. The primary end point used indicate effectiveness rate change according...