A5018384292- Metabolomics and Mass Spectrometry Studies
- Analytical Methods in Pharmaceuticals
- Traditional Chinese Medicine Analysis
- Antibiotics Pharmacokinetics and Efficacy
- Berberine and alkaloids research
- Paraquat toxicity studies and treatments
- Plant-based Medicinal Research
- Analytical Chemistry and Chromatography
- Phytochemistry and Biological Activities
- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Sulfur Compounds in Biology
- Pharmacological Effects and Assays
- Pharmacogenetics and Drug Metabolism
- Alkaloids: synthesis and pharmacology
- Hippo pathway signaling and YAP/TAZ
- Alcohol Consumption and Health Effects
- Drug Transport and Resistance Mechanisms
- Tryptophan and brain disorders
- Chronic Myeloid Leukemia Treatments
- Treatment of Major Depression
- Synthesis and bioactivity of alkaloids
- Chromatography in Natural Products
- Drug-Induced Hepatotoxicity and Protection
- Ginseng Biological Effects and Applications
Wenzhou Medical University
2016-2025
Institute of Forensic Science
2015-2021
Molina Center for Energy and the Environment
2015
First Affiliated Hospital of Wenzhou Medical University
2013-2015
Analysis and Testing Centre
2015
Wenzhou University
2013
Zero to Three
2011
Abstract The early identification of toxic paraquat ( PQ ) poisoning in patients is critical to ensure timely and accurate prognosis. Although plasma concentration has been reported as a clinical indicator poisoning, it not commonly applied practice due the inconvenient necessary instruments operation. In this study, we explored use blood routine indexes identify degree toxicity and/or diagnose via machine learning approach. Specifically, developed method based on support vector combined...
Abstract A specific ultra‐performance liquid chromatography tandem mass spectrometry method is described for the simultaneous determination of bupropion, metroprolol, midazolam, phenacetin, omeprazole and tolbutamide in rat plasma with diazepam as internal standard, which are six probe drugs cytochrome P450 isoforms CYP2B6, CYP2D6, CYP3A4, CYP1A2, CYP2C19 CYP2C9. Plasma samples were protein precipitated acetonitrile. The chromatographic separation was achieved using a UPLC® BEH C 18 column...
Globally, rodents and shrew populations constitute crucial elements of diverse environments animal communities. It is imperative to study their population dynamics mitigate any potential negative impact on humans, as they can be involved in the transmission critical zoonotic agents, such
Acetylcorynoline is an alkaloid isolated from the tubers of Corydalis ambigua Cham. et Schltdl. It has anti‐inflammatory properties with potential to treat Parkinson’s disease. However, use UPLC–MS/MS for identifying acetylcorynoline in mouse plasma not yet been explored. The present study aimed develop a fast and selective method determining amount using UPLC–MS/MS. Plasma samples (10 μL) were prepared methanol‐induced protein precipitation following addition aconitine as internal standard....
Abstract Violanthin, a compound isolated from the stems of Dendrobium officinale , exhibits potent antioxidant and antibacterial activities. However, no research has been conducted on identification violanthin in mouse plasma using UPLC-MS/MS. The present study aims to develop selective UPLC-MS/MS method for quantification plasma. Samples were prepared acetonitrile protein precipitation, with aconitine as an internal standard (IS). Chromatographic separation was achieved UPLC HSS T3 column...
Abstract In this experiment, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was employed to quantify karacoline in mouse plasma following both intravenous and oral administration, thereby elucidating the pharmacokinetic characteristics of mice. The analytes were extracted from using acetonitrile for protein precipitation. Chromatographic separation performed on an HSS T3 column via gradient elution, with mobile phase consisting methanol 0.1% formic acid water....
Numerous people die of paraquat (PQ) poisoning every year in the world. Although several studies regarding have been conducted, metabolic changes plasma remain unknown. In this study, metabolomics 15 PQ poisoned patients with concentrations excess 0.1 µg/mL and 16 healthy volunteers were investigated. The samples evaluated through use gas chromatography-mass spectrometry (GC/MS) analyzed by partial least-squares discriminant analysis (PLS-DA). Based on data, a support vector machine (SVM)...
Dendrobine, considered as the major active alkaloid compound, has been used for quality control and discrimination of Dendrobium which is documented in Chinese Pharmacopoeia. In this work, a sensitive simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method determination dendrobine rat plasma developed. After addition caulophyline an internal standard (IS), protein precipitation by acetonitrile-methanol (9:1, v/v) was to prepare samples. Chromatographic...
In this study, we developed a brain metabolomic method, based on gas chromatography-mass spectrometry (GC/MS), to evaluate the effect of ketamine rats. Pattern recognition analysis, including both principal component analysis and partial least squares-discriminate revealed that induced metabolic perturbations. Compared with control group, levels glycerol, uridine, cholesterol in rat group (50 mg/kg, 14 days) decreased, while urea increased. Our results indicate methods GC/MS may be useful...
Abstract Decellularization processes may variably distort or degrade extracellular matrix (ECM) structure. In this study, two perfusion routes (PR) were tested on SD rat lung samples. One decellularization protocol, PR1, was perfused through the pulmonary artery. The other PR2, trachea. Both protocols used by same detergent‐based (sodium dodecyl sulphate and Triton X‐100) with different flow continuous perfusion. There no visible difference in vessel architecture between PR1‐...
Hydrogen sulfide (H2S) is the second leading cause of toxin-related deaths in operational site. Its main target organs toxic effects are central nervous system and respiratory system. In this study, we developed a serum metabonomic method, based on gas chromatography-mass spectrometry (GC/MS), to evaluate effect acute poisoning by hydrogen rats. Pattern recognition analysis, including both principal component analysis (PCA) partial least squares-discriminate (PLS-DA), revealed that induced...
Paraquat is one of the most widely used herbicides in world and highly toxic to humans animals. In this study, we developed a serum metabolomic method based on GC/MS evaluate effects acute paraquat poisoning rats. Pattern recognition analysis, including both principal component analysis partial least squares-discriminate revealed that induced metabolic perturbations. Compared with control group, level octadecanoic acid, L-serine, L-threonine, L-valine, glycerol group (36 mg/kg) increased,...
The rats were randomly divided into paraquat group, curcumin treatment and pirfenidone group. concentration of in rat plasma was determined by an ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method over the range 10–2000 ng mL −1 . Chromatographic separation achieved on a BEH HILIC (2.1 mm × 100 mm, 1.7 μm) column. mobile phase consisted acetonitrile 10 ammonium formate buffer (containing 0.1% formic acid) with gradient elution pumped at flow rate 0.4 min...
Most paraquat (PQ) poisoned patients died from acute multiple organ failure (MOF) such as lung, kidney, and heart. However, the exact mechanism of intoxication is still unclear. In order to find out initial toxic PQ poisoning, a blood metabolomics study based on ultraperformance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) efficient machine learning approach was performed 23 29 healthy subjects. The plasma concentrations were >1000 ng/mL, samples...