A5101778075- Antibiotics Pharmacokinetics and Efficacy
- Metabolomics and Mass Spectrometry Studies
- Analytical Methods in Pharmaceuticals
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Plant-based Medicinal Research
- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Paraquat toxicity studies and treatments
- Chronic Myeloid Leukemia Treatments
- Traditional Chinese Medicine Analysis
- Sulfur Compounds in Biology
- Analytical Chemistry and Chromatography
- Chronic Lymphocytic Leukemia Research
- Tryptophan and brain disorders
- Pneumocystis jirovecii pneumonia detection and treatment
- Analytical Chemistry and Sensors
- Ginseng Biological Effects and Applications
- Biochemical Analysis and Sensing Techniques
- Pharmacological Effects of Natural Compounds
- Alcohol Consumption and Health Effects
- Antifungal resistance and susceptibility
- Pesticide Residue Analysis and Safety
- Pharmacological Effects and Assays
- Drug-Induced Hepatotoxicity and Protection
Wenzhou Medical University
2015-2025
First Affiliated Hospital of Wenzhou Medical University
2015-2025
Several mechanisms underlying nephrolithiasis, one of the most common urological diseases, involve calcium oxalate formation, including oxidative stress, inflammatory reactions, fibrosis, pyroptosis, and apoptosis.
In the current research experiment, a sensitive, precise and rapid bioanalytical approach involving detection of fedratinib concentrations in rat plasma by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) technique was optimized established, it employed to describe changes after oral treatment with various antifungal drugs (isavuconazole, posaconazole, fluconazole itraconazole). An Acquity UPLC BEH reverse-phase C18 column (2.1 mm × 50 mm, 1.7 μm) used for...
Current FDA-approved label recommends that the dosage of polymyxin B should be adjusted according to renal function. However, correlation between pharmacokinetics (PK) and creatinine clearance (CrCL) is poor. This study aimed develop a population PK model in adult patients with various functions identify dosing strategy.A retrospective was performed 32 Nonlinear mixed effects modelling applied build followed by Monte Carlo simulations which designed regimens across function.Polymyxin...
Background: Presently, colistin is commercially available in two different forms, namely, sulfate and its sulphomethylated derivative, colistimethate sodium (CMS). However, the currently reported studies, most of clinical studies on for parenteral use are referred to as CMS. Data pharmacokinetics (PK), efficacy, side effects have not been reported. Methods: This retrospective study was performed carbapenem-resistant organism (CRO)-infected patients treated with more than 72 h. The population...
Abstract A specific ultra‐performance liquid chromatography tandem mass spectrometry method is described for the simultaneous determination of bupropion, metroprolol, midazolam, phenacetin, omeprazole and tolbutamide in rat plasma with diazepam as internal standard, which are six probe drugs cytochrome P450 isoforms CYP2B6, CYP2D6, CYP3A4, CYP1A2, CYP2C19 CYP2C9. Plasma samples were protein precipitated acetonitrile. The chromatographic separation was achieved using a UPLC® BEH C 18 column...
Omadacycline is a novel aminomethylcycline antibiotic that retains its antibacterial activity against strain-specific efflux pumps and ribosomal protective protein mechanisms of tetracycline resistance. To determine the concentration omadacycline in human plasma, an ultra-high-performance liquid chromatography-tandem mass spectrometry method was developed to provide basis for therapeutic monitoring clinical settings. The experimental approach involves using ACQUITY UPLC BEH C18 column (2.1 ×...
Numerous people die of paraquat (PQ) poisoning every year in the world. Although several studies regarding have been conducted, metabolic changes plasma remain unknown. In this study, metabolomics 15 PQ poisoned patients with concentrations excess 0.1 µg/mL and 16 healthy volunteers were investigated. The samples evaluated through use gas chromatography-mass spectrometry (GC/MS) analyzed by partial least-squares discriminant analysis (PLS-DA). Based on data, a support vector machine (SVM)...
Background: Linezolid is associated with myelosuppression, which may cause failure in optimally treating bacterial infections. The study aimed to define the pharmacokinetic/toxicodynamic (PK/TD) threshold for critically ill patients and identify a dosing strategy renal insufficiency. Methods: population pharmacokinetic (PK) model was developed using NONMEM program. Logistic regression modeling conducted determine toxicodynamic (TD) of linezolid-induced myelosuppression. regimen optimized...
In this study, we developed a brain metabolomic method, based on gas chromatography-mass spectrometry (GC/MS), to evaluate the effect of ketamine rats. Pattern recognition analysis, including both principal component analysis and partial least squares-discriminate revealed that induced metabolic perturbations. Compared with control group, levels glycerol, uridine, cholesterol in rat group (50 mg/kg, 14 days) decreased, while urea increased. Our results indicate methods GC/MS may be useful...
Aims Data regarding clinical pharmacokinetic/toxicodynamic (PK/TD) of polymyxin B is short direct quantitative data. This study aims to investigate the risk factors associated acute kidney injury (AKI) and assess relationship between plasma levels its nephrotoxicity. Methods A retrospective was performed in adult patients treated with B. Risk AKI trough concentrations were identified via medical record review. multivariate logistic regression model established B‐associated predicted by a...
Paraquat is one of the most widely used herbicides in world and highly toxic to humans animals. In this study, we developed a serum metabolomic method based on GC/MS evaluate effects acute paraquat poisoning rats. Pattern recognition analysis, including both principal component analysis partial least squares-discriminate revealed that induced metabolic perturbations. Compared with control group, level octadecanoic acid, L-serine, L-threonine, L-valine, glycerol group (36 mg/kg) increased,...
Most paraquat (PQ) poisoned patients died from acute multiple organ failure (MOF) such as lung, kidney, and heart. However, the exact mechanism of intoxication is still unclear. In order to find out initial toxic PQ poisoning, a blood metabolomics study based on ultraperformance liquid chromatography coupled quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) efficient machine learning approach was performed 23 29 healthy subjects. The plasma concentrations were >1000 ng/mL, samples...
Paraquat is quick-acting and non-selective, killing green plant tissue on contact; it also toxic to human beings animals. In this study, we developed a urine metabonomic method by gas chromatography-mass spectrometry evaluate the effect of acute paraquat poisoning rats. Pattern recognition analysis, including both partial least squares discriminate analysis principal component revealed that induced metabolic perturbations. Compared with control group, levels benzeneacetic acid hexadecanoic...
Limited therapeutic options exist for multidrug-resistant/extensively drug-resistant Acinetobacter baumannii (MDR/XDR-Ab) meningitis/ventriculitis. A combination of intravenous and intraventricular (IVT)/intrathecal (IT) polymyxins achieves good outcomes cases healthcare-associated MDR/XDR-Ab Colistin is commercially available as colistin sulphate its sulphomethylated derivative. However, the effect safety in treatment meningitis/ventriculitis has not been reported. We report on a...
In order to investigate the effect of hemoperfusion (HP) on clearance rate paraquat (PQ) and develop a model, 41 PQ-poisoned patients who acquired acute PQ intoxication received HP treatment. concentrations were determined by high performance liquid chromatography (HPLC). According initial concentration, study subjects divided into two groups: Low-PQ group (0.05–1.0 μ g/mL) High-PQ (1.0–10 g/mL). After treatment, decreased in both groups. However, group, levels remained excess 0.05 g/mL...
Gas chromatography-mass spectrometry (GS-MS) in combination with multivariate statistical analysis was applied to explore the metabolic variability urine of chronically hydrogen sulfide- (H 2 S-) poisoned rats relative control ones. The changes endogenous metabolites were studied by partial least squares-discriminate (PLS-DA) and independent-samples t -test. patterns H S-poisoned group are separated from control, suggesting that profiles markedly different controls. Moreover, compared group,...