A5081830070- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Liver Disease Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- Kruppel-like factors research
- TGF-β signaling in diseases
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Bone Metabolism and Diseases
- Cancer, Lipids, and Metabolism
- Metabolism, Diabetes, and Cancer
- Cardiac Arrest and Resuscitation
- RNA Interference and Gene Delivery
- Fibroblast Growth Factor Research
- ATP Synthase and ATPases Research
- Lipid metabolism and biosynthesis
- Drug Transport and Resistance Mechanisms
- RNA Research and Splicing
- Ion Channels and Receptors
- Cell death mechanisms and regulation
- Plant biochemistry and biosynthesis
- Nitric Oxide and Endothelin Effects
Xinxiang Medical University
2023-2025
Xiamen University
2024-2025
China Pharmaceutical University
2022-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Yangtze University
2021-2024
Virginia Tech
2021-2024
Huazhong University of Science and Technology
2024
Tongji Hospital
2024
Nanjing University
2013-2024
China-Japan Friendship Hospital
2024
Abstract Lipid overload results in lipid redistribution among metabolic organs such as liver, adipose, and muscle; therefore, the interplay between liver other is important to maintain homeostasis. Here, we show that responds first sends hepatocyte-derived extracellular vesicles (EVs) targeting adipocytes regulate adipogenesis lipogenesis. Geranylgeranyl diphosphate synthase (Ggpps) expression enhanced by regulates EV secretion through Rab27A geranylgeranylation. Consistently, liver-specific...
Most existing studies focus on either the relationship between sarcopenia and depressive symptoms or cognitive functions. Few have examined functions simultaneously. This study aimed to analyze both in elderly by introducing activities of daily living explore mediating role mental health. used data from 2015 2018 China Health Retirement Longitudinal Study, including a total 3,552 older adults aged 60 above. We conducted descriptive statistical analysis correlation seemingly unrelated...
We have developed a new transporter structure that provides effective delivery of Morpholino antisense oligomers into wide variety tissues in living mice. This comprises dendritic assembled around triazine core which serves to position eight guanidinium head groups conformation penetrate cell membranes. is conjugated oligomer form delivery-enabled product referred as Vivo-Morpholino. Vivo-Morpholinos are shown effectively enter and function within cultured cells the presence 100% serum using...
Abstract Background Connective tissue growth factor (CTGF) is a highly profibrogenic molecule implicated in hepatic fibrogenesis. Small interfering RNA (siRNA) an effective tool to silence gene expression post‐transcriptionally. Therefore, we conducted investigation determine if intraportal vein siRNA injection targeting CTGF inhibits on rat liver vivo and furthermore whether it protects the from fibrosis. Methods Some rats received carbon tetrachloride (CCl 4 ) by subcutaneous injections...
Endoplasmic reticulum (ER) stress activates the adaptive unfolded protein response (UPR) and represents a critical mechanism that underlies metabolic dysfunctions. Fibroblast growth factor 21 (FGF21), hormone is predominantly secreted by liver, exerts broad range of effects upon metabolism carbohydrates lipids. Although increased circulating levels FGF21 have been documented in animal models human subjects with obesity nonalcoholic fatty liver disease, functional interconnections between ER...
We report a role for the mitochondrial single-stranded DNA binding protein (mtSSB) in regulating (mtDNA) replication initiation mammalian mitochondria. Transcription from light-strand promoter (LSP) is required both gene expression and generating RNA primers needed of mtDNA synthesis. In absence mtSSB, transcription LSP strongly up-regulated, but no are formed. Using deep sequencing mouse knockout model biochemical reconstitution experiments with pure proteins, we find that mtSSB necessary...
Abstract Heart development is a continuous process involving significant remodeling during embryogenesis and neonatal stages. To date, several groups have used single-cell sequencing to characterize the heart transcriptomes but failed capture progression of at most This has left gaps in understanding contribution each cell type across cardiac development. Here, we report transcriptional profile murine from early late Through further analysis this dataset, identify features. We gene...
Abstract Heart organoids have the potential to generate primary heart-like anatomical structures and hold great promise as in vitro models for cardiac disease. However, their properties not yet been fully studied, which hinders wide spread application. Here we report development of differentiation systems ventricular atrial heart organoids, enabling study diseases with chamber defects. We show that our chamber-specific comprising major cell types, use single RNA sequencing together sample...
Atopic dermatitis (AD) is one of the most common chronic inflammatory cutaneous diseases with unmet clinical needs. As a ingredient found in several medicinal herbs efficacy on diseases, Scutellarein (Scu) has been shown to possess anti-inflammatory and anti-proliferative activities. We aimed evaluate therapeutic Scu against AD its underlying molecular mechanism.Efficacy was evaluated 2,4-dinitrofluorobenzene (DNFB) carvacrol-induced mouse models. Cytokine mRNA serum IgE levels were examined...
Abstract The oxidative phosphorylation system 1 in mammalian mitochondria plays a key role transducing energy from ingested nutrients 2 . Mitochondrial metabolism is dynamic and can be reprogrammed to support both catabolic anabolic reactions, depending on physiological demands or disease states. Rewiring of mitochondrial intricately linked metabolic diseases promotes tumour growth 3–5 Here, we demonstrate that oral treatment with an inhibitor transcription (IMT) 6 shifts whole-animal...
The probiotic Escherichia coli Nissle (EcN) is an exceptional strain that has attracted significant attention not only for its clinical efficacy in the treatment and prevention of gastrointestinal disorders but also as a burgeoning microbial chassis living therapeutic applications. However, there immediate necessity to develop conditional expression systems confine activity EcN specifically tract, avoid influencing environment. Here, we constructed two genetically encoded interchangeable...
Background and Purpose Genetic ablation or inhibition of the cation channel TRPC6 is protective against renal, cardiac intestinal fibrosis. However, expression decreased in patients with liver diseases. Here, we explored role fibrosis underlying mechanism. Experimental Approach Bile duct ligation thioacetamide gavage were used to model C57BL/6J mice. Western blotting, immunolabelling qPCR employed for protein mRNA expression. Liver injury/fibrosis assessed using serum alanine transaminase...
Article29 April 2019Open Access Transparent process TEFM regulates both transcription elongation and RNA processing in mitochondria Shan Jiang orcid.org/0000-0003-2020-6447 Department of Medical Biochemistry Biophysics, Karolinska Institutet, Stockholm, Sweden Max Planck Institute Biology Ageing - Institutet Laboratory, Search for more papers by this author Camilla Koolmeister Jelena Misic Stefan Siira Harry Perkins Research Centre Research, The University Western Australia, Perth, WA,...
Abstract Mutations in the microtubule-associated protein tau ( MAPT ) gene cause autosomal dominant frontotemporal lobar degeneration with inclusions (FTLD-tau). p.R406W carriers present clinically progressive memory loss and neuropathologically neuronal glial tauopathy. However, pathogenic events triggered by expression of mutant remain poorly understood. To identify genes pathways that are dysregulated FTLD-tau, we performed transcriptomic analyses induced pluripotent stem cell...
Mumps commonly affects children 5–9 yr of age, and can lead to permanent adult sterility in certain cases. However, the etiology this long-term effect remains unclear. infection results progressive degeneration seminiferous epithelium and, occasionally, Sertoli cell–only syndrome. Thus, remaining cells may be critical spermatogenesis recovery after orchitis healing. Here, we report that protein farnesylation/geranylgeranylation balance is for patients’ fertility. The expression...
BackgroundFew data are available on the clinical features and outcomes of Chinese patients with systemic immunoglobulin light-chain (AL) amyloidosis. The aim this study is to reveal picture risk factors disease progression in a large cohort AL
Abstract Patients with obesity have a high prevalence of non‐alcoholic fatty liver disease (NAFLD) and, in parallel, increased susceptibility to fibrosis/cirrhosis/hepatocellular carcinoma (HCC). Herein, we report that high‐fat diet (HFD) can augment glycolysis and then accelerate NAFLD–fibrosis progression by downregulating the expression geranylgeranyl diphosphate synthase (GGPPS), which is critical enzyme mevalonate pathway. Long‐term HFD overloading decreases GGPPS mice, shifts fuel...
Regorafenib is a potent multikinase inhibitor for the second-line targeted therapy against hepatocellular carcinoma (HCC); however, drug resistance emerging in clinical settings. Although cancer stem cells (CSCs) are considered as key determinate of sensitivity, it remains unclear how CSCs may communicate with differentiated counterparts (non-CSC) to dictate therapeutic efficacy. Therefore, we sought investigate regorafenib mechanism HCC.We used sphere formation and soft agar colony assays...
Abstract Loss of first‐phase insulin secretion associated with β cell dysfunction is an independent predictor type 2 diabetes mellitus ( T2DM ) onset. Here we found that a critical enzyme involved in protein prenylation, geranylgeranyl pyrophosphate synthase GGPPS ), required to maintain secretion. shows biphasic expression pattern islets db/db mice during the progression : increased compensatory period, followed by decrease dysfunction. Ggpps deletion cells results typical dysfunction,...