A5012036567- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Lipid metabolism and biosynthesis
- Cardiovascular Disease and Adiposity
- Exercise and Physiological Responses
- Metabolism, Diabetes, and Cancer
- Diet, Metabolism, and Disease
- Peroxisome Proliferator-Activated Receptors
- Regulation of Appetite and Obesity
- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Single-cell and spatial transcriptomics
- Nuclear Receptors and Signaling
- Diabetes and associated disorders
- Diet and metabolism studies
- Thermoregulation and physiological responses
- Dietary Effects on Health
- Fibroblast Growth Factor Research
- Nutrition and Health in Aging
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Birth, Development, and Health
- Nerve injury and regeneration
Chinese Academy of Sciences
2010-2023
Institut Pasteur of Shanghai
2022-2023
The University of Texas Health Science Center at Houston
2023
The University of Texas Southwestern Medical Center
2014-2022
The University of Texas at Dallas
2018
Southwestern Medical Center
2017
Shenzhen Pingle Orthopedic Hospital
2016
Shanghai Institutes for Biological Sciences
2010-2015
Nutrition Sciences (Belgium)
2011
University of Chinese Academy of Sciences
2010
Although regeneration through the reprogramming of one cell lineage to another occurs in fish and amphibians, it has not been observed mammals. We discovered mouse that during wound healing, adipocytes regenerate from myofibroblasts, a type thought be differentiated nonadipogenic. Myofibroblast required neogenic hair follicles, which triggered bone morphogenetic protein (BMP) signaling then activation adipocyte transcription factors expressed development. Overexpression BMP antagonist Noggin...
White adipose tissue (WAT) remodeling is dictated by coordinated interactions between adipocytes and resident stromal-vascular cells; however, the functional heterogeneity of stromal cells has remained unresolved. We combined single-cell RNA-sequencing FACS to identify isolate functionally distinct subpopulations PDGFRβ+ within visceral WAT adult mice. LY6C- CD9- represent highly adipogenic adipocyte precursor ('APCs'), whereas LY6C+ fibro-inflammatory progenitors ('FIPs'). FIPs lack...
Brown adipose tissue (BAT), as the main site of adaptive thermogenesis, exerts beneficial metabolic effects on obesity and insulin resistance. BAT has been previously assumed to contain a homogeneous population brown adipocytes. Utilizing multiple mouse models capable genetically labeling different cellular populations, well single-cell RNA sequencing 3D profiling, we discovered adipocyte subpopulation with low thermogenic activity coexisting classical high-thermogenic adipocytes within BAT....
Dermal adipose tissue (also known as dermal white and herein referred to dWAT) has been the focus of much discussion in recent years. However, dWAT remains poorly characterized. The fate mature adipocytes origin rapidly reappearing at different stages remain unclear. Here, we isolated characterized fat cellular molecular level. Together with dWAT's dynamic responses external stimuli, established that are a distinct class high plasticity. By combining pulse-chase lineage tracing single-cell...
Misalignment of feeding rhythms with the light-dark cycle leads to disrupted peripheral circadian clocks and obesity. Conversely, restricting active period mitigates metabolic syndrome through mechanisms that remain unknown. We found genetic enhancement adipocyte thermogenesis ablation zinc finger protein 423 (ZFP423) attenuated obesity caused by consumption a high-fat diet during inactive (light) increasing futile creatine cycling in mice. Circadian control metabolism underlies timing...
Endoplasmic reticulum (ER) stress activates the adaptive unfolded protein response (UPR) and represents a critical mechanism that underlies metabolic dysfunctions. Fibroblast growth factor 21 (FGF21), hormone is predominantly secreted by liver, exerts broad range of effects upon metabolism carbohydrates lipids. Although increased circulating levels FGF21 have been documented in animal models human subjects with obesity nonalcoholic fatty liver disease, functional interconnections between ER...
RACK1 dictates the response of intracellular stress sensor IRE1α to different extracellular stimuli.
Activated beige adipocytes have therapeutic potential due to their ability improve glucose and lipid homeostasis. To date, the origin of remains enigmatic. Whether cells arise through de novo differentiation from resident precursors or reprogramming mature white has been a topic intense discussion. Here, we offer our perspective on natural in mice. In particular, revisit recent lineage-tracing studies that shed light this issue new insight into how environmental housing temperatures early...
Pathologic expansion of white adipose tissue (WAT) in obesity is characterized by adipocyte hypertrophy, inflammation, and fibrosis; however, factors triggering this maladaptive remodeling are largely unknown. Here, we test the hypothesis that potential to recruit new adipocytes from Pdgfrβ+ preadipocytes determines visceral WAT health obesity. We manipulate levels Pparg, master regulator adipogenesis, precursors adult mice. Increasing adipogenic capacity through Pparg overexpression results...
The selective estrogen receptor modulator tamoxifen, in combination with the Cre-ER(T2) fusion protein, has been one of mainstream methods to induce genetic recombination and found widespread application lineage tracing studies.Here, we report that tamoxifen exposure at widely used concentrations remains detectable by mass-spectrometric analysis adipose tissue after a washout period 10 days. Surprisingly, its ability maintain nuclear translocation protein is preserved beyond 2 months...
Starting at middle age, adults often suffer from visceral adiposity and associated adverse metabolic disorders. Lineage tracing in mice revealed that adipose progenitor cells (APCs) fat undergo extensive adipogenesis during age. Thus, despite the low turnover rate of adipocytes young adults, is unlocked Transplantations quantitatively showed APCs middle-aged exhibited high adipogenic capacity cell-autonomously. Single-cell RNA sequencing identified a distinct APC population, committed...
As an adipokine in circulation, adiponectin has been extensively studied for its beneficial metabolic effects. While many important functions have attributed to under high-fat diet conditions, little is known about essential role regular chow. Employing a mouse model with inducible, acute β-cell ablation, we uncovered of insulinopenic conditions maintain minimal lipid homeostasis. When insulin levels are marginal, critical signaling, endocytosis, and uptake subcutaneous white adipose tissue....
The endoplasmic reticulum (ER)-resident protein kinase/endoribonuclease inositol-requiring enzyme 1 (IRE1) is activated through transautophosphorylation in response to folding overload the ER lumen and maintains homeostasis by triggering a key branch of unfolded response. Here we show that mammalian IRE1α liver cells also phosphorylated kinase other than itself metabolic stimuli. Glucagon-stimulated PKA, which turn at Ser 724 , highly conserved site within activation domain. Blocking...