A5049696513- Hair Growth and Disorders
- Mesenchymal stem cell research
- Skin and Cellular Biology Research
- Wnt/β-catenin signaling in development and cancer
- Wound Healing and Treatments
- Endodontics and Root Canal Treatments
- Periodontal Regeneration and Treatments
- Cardiac Arrest and Resuscitation
- Cancer and Skin Lesions
- Bone and Dental Protein Studies
- Bone Metabolism and Diseases
- Venomous Animal Envenomation and Studies
- Simulation-Based Education in Healthcare
- Epigenetics and DNA Methylation
- dental development and anomalies
- Tissue Engineering and Regenerative Medicine
- Pregnancy and preeclampsia studies
- Nerve injury and regeneration
- Congenital heart defects research
- Rabies epidemiology and control
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- PARP inhibition in cancer therapy
- Single-cell and spatial transcriptomics
- Traditional Chinese Medicine Analysis
University of Pennsylvania
2013-2025
Integrated Chinese Medicine (China)
2023-2025
Capital Medical University
2009-2025
Chinese PLA General Hospital
2024-2025
Chinese People's Liberation Army
2024-2025
Capital University
2021-2025
Fuzhou Second Hospital
2024
China-Japan Friendship Hospital
2022-2024
University of Chinese Academy of Sciences
2023
Sichuan University
2023
Periodontitis is a periodontal tissue infectious disease and the most common cause for tooth loss in adults. It has been linked to many systemic disorders, such as coronary artery disease, stroke, diabetes. At present, there no ideal therapeutic approach cure periodontitis achieve optimal regeneration. In this study, we explored potential of using autologous ligament stem cells (PDLSCs) treat defects porcine model periodontitis. The lesion was generated first molars area miniature pigs by...
Although regeneration through the reprogramming of one cell lineage to another occurs in fish and amphibians, it has not been observed mammals. We discovered mouse that during wound healing, adipocytes regenerate from myofibroblasts, a type thought be differentiated nonadipogenic. Myofibroblast required neogenic hair follicles, which triggered bone morphogenetic protein (BMP) signaling then activation adipocyte transcription factors expressed development. Overexpression BMP antagonist Noggin...
During normal development, the epidermis and hair follicle are distinct lineage compartments maintained by independent stem cell populations. Both epidermal follicular keratinocytes recruited to participate in repair response injury. However, it is generally thought that cells contribute wound only transiently ultimately replaced progeny of derived from original compartment prior wounding. Here we use inducible constitutive cre recombinase expressed Sonic hedgehog locus (Shh) for vivo...
Mammalian wounds typically heal by fibrotic repair without hair follicle (HF) regeneration. Fibrosis and regeneration are currently considered the opposite end of wound healing. This study sought to determine if scar could be remodeled promote healing with HF Here, we identify that activation Sonic hedgehog (Shh) pathway reinstalls a regenerative dermal niche, called papilla, which is required sufficient for neogenesis (HFN). Epidermal Shh overexpression or constitutive Smoothened results in...
Stem cells from human exfoliated deciduous teeth have been identified as a new post-natal stem cell population with multipotential differentiation capabilities, including regeneration of mineralized tissues in vivo. To examine the efficacy utilizing these regenerating orofacial bone defects, we isolated miniature pig and engrafted critical-size defects generated swine mandible models. Our results indicated that teeth, an autologous easily accessible source, were able to engraft regenerate...
Adult mammalian wounds, with rare exception, heal fibrotic scars that severely disrupt tissue architecture and function. Regenerative medicine seeks methods to avoid scar formation restore the original structures. We show in three adult mouse models pharmacologic activation of nociceptor TRPA1 on cutaneous sensory neurons reduces can also promote regeneration. Local induces regeneration distant untreated areas injury, demonstrating a systemic effect. Activated stimulates local production...
The goal of regenerative endodontics is to restore the functions dental pulp–dentin complex. Two approaches are being applied toward regeneration: cell transplantation and homing. majority previous based on by delivering ex vivo cultivated cells pulp or dentin regeneration. Many hurdles limit clinical translation such as difficulty acquiring isolating viable cells, uncertainty what fractions use, excessive cost manipulation transportation, risk immune rejection, pathogen transmission,...
Approximately 50% of high-grade serous ovarian cancers (HGSOCs) have defects in genes involved homologous recombination (HR) (i.e., BRCA1/2). Preclinical models to optimize therapeutic strategies for HR-deficient (HRD) HGSOC are lacking. We developed a preclinical platform HRD HGSOCs that includes primary tumor cultures, patient-derived xenografts (PDXs), and molecular imaging. Models were characterized by immunohistochemistry, targeted sequencing, reverse-phase protein array analysis. also...
Using an interwoven-loop experimental design in conjunction with highly conservative linear mixed model methodology using estimated variance components, 18 genes differentially expressed between nuclear transfer (NT)- and vitro fertilization (IVF)-produced embryos were identified. The set is comprised of three intermediate-filament protein (cytokeratin 8, cytokeratin 19, vimentin), metabolic (phosphoribosyl pyrophosphate synthetase 1, mitochondrial acetoacetyl-coenzyme A thiolase,...
Reparative dentin formation is essential for maintaining the integrity of structure during disease or trauma. In this study, we investigated stem/progenitor cell-based tissue engineering regeneration in a large animal model. Porcine deciduous pulp cells (PDPSCs) were mixed with beta-tricalcium phosphate (β-TCP) scaffold regeneration. Different concentrations PDPSCs tested to determine optimal density Aliquots 5×10 5 1 mL resulted highest number attached and greatest alkaline phosphatase...
Epidemiological studies have reported a higher incidence of growth disorders among newborns conceived by in vitro fertilization (IVF), suggesting that IVF may be disruptive to the process embryonic and fetal growth. However, long-term effects on molecular mechanisms remain unclear. Therefore, we evaluated body weight mice from birth age 1.5 yr. In addition, analyzed gene expression insulin-like factor 2 (Igf2), H19, Igf2 receptor (Igf2r), miR-483 their DNA methylation status using real-time...
To explore the long-term effect of intelligent first-aid training based on virtual reality (VR) technology cardiopulmonary resuscitation (CPR) skill proficiency. The convenience sampling method was used to select a total 100 non-medical volunteers from Nanchang, China, and this cohort randomized either VR group (VR group) or traditional simulation scenario (traditional group). Relevant data were collected for comparative analysis. Participants evaluated by measuring mean chest compression...
Most wounds form scars without hair follicles. However, in the wound-induced neogenesis (WIHN) model of skin regeneration, regenerate follicles if tissue rigidity is optimal. Although WIHN depends on Wnt signaling, whether performs a mechanoregulatory role that contributes to regeneration remains uncharacterized. Here, we demonstrate signaling affects mechanosensitivity at both cellular and levels drive WIHN. Atomic force microscopy revealed an attenuated substrate response epidermal but not...
Antibody-based technology is the main method for diagnosis and treatment of snake bite envenoming currently. However, development an antibody, polyclonal or monoclonal, a complicated costly procedure. Aptamers are single stranded oligonucleotides that recognize specific targets such as proteins have shown great potential over years diagnostic therapeutic agents. In contrast to antibodies, aptamers can be selected in vitro without immunization animals, synthesized chemically with extreme...
The dynamics of the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) breakthrough infections remain unclear, particularly when compared responses in naive individuals. In this longitudinal prospective cohort study, 13 participants were recruited. Peripheral blood samples collected every other day until 7 after symptom onset. Transcriptome sequencing, single-cell T-cell receptor (TCR) B-cell (BCR) Olink proteomics, and antigen-antibody binding experiments then...
Abstract The hair follicle is considered to be a model system for studying organogenesis. In our initial study using mouse cells (Zheng et al., 2005) we found that new formation always starts from an epithelial platform: the epidermal aggregate, aggregates encyst, and periphery of cysts, centrifugally, buds, pegs, follicles form. this report, extend four distantly related mammals: opossum, rat, dog human. We find in these species, plus mouse, most trichogenic are earliest stages development...
Dentin in permanent teeth rarely undergoes resorption development, homeostasis, or aging, contrast to bone that periodic resorption/remodeling. The authors hypothesized cells the mesenchymal compartment of dental pulp attenuate osteoclastogenesis. Mononucleated and adherent from donor-matched rat (dental [DPCs]) alveolar (alveolar [ABCs]) were isolated separately cocultured with primary splenocytes. Primary splenocytes readily aggregated formed osteoclast-like chemically defined...