A5089429406- Cancer-related molecular mechanisms research
- Angiogenesis and VEGF in Cancer
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Axon Guidance and Neuronal Signaling
- Zebrafish Biomedical Research Applications
- Traumatic Brain Injury Research
- Pancreatic function and diabetes
- MicroRNA in disease regulation
- Liver physiology and pathology
- Traumatic Brain Injury and Neurovascular Disturbances
- 3D Printing in Biomedical Research
- ATP Synthase and ATPases Research
- Environmental Quality and Pollution
- Tuberculosis Research and Epidemiology
- Land Use and Ecosystem Services
- Metabolism and Genetic Disorders
- Hemoglobinopathies and Related Disorders
- Genetic and Kidney Cyst Diseases
- Heme Oxygenase-1 and Carbon Monoxide
- Diet and metabolism studies
- Renal and related cancers
- Online and Blended Learning
- Autophagy in Disease and Therapy
- Synthesis and Biological Activity
Medical College of Wisconsin
2013-2025
Children's Hospital of Wisconsin
2010-2019
Hebei University of Environmental Engineering
2017-2019
Wuhan University
2009
Children's Research Hospital
2008
Deutsch Amerikanisches Institut Saarland
2006
Chinese Academy of Sciences
2004-2006
Institute of Zoology
2004-2006
The plasmacytoma variant translocation 1 gene (PVT1) is an lncRNA that has been designated as oncogene due to its contribution the phenotype of multiple cancers. Although mechanism by which PVT1 influences disease processes studied in cancer types, role cervical tumorigenesis remains unknown. Thus, present study was designed investigate vitro and vivo. expression measured quantitative PCR (qPCR) 121 invasive carcinoma (ICC) samples, 30 normal cervix cell lines. Functional assays were carried...
Necrotizing enterocolitis (NEC) is a severe form of bowel disease that develops in premature infants. Although animal data and human studies suggest aberrant activation the intestinal immune system contributes to NEC, pathogenesis remains unclear. We hypothesized inherited defects regulation Toll-like receptor signaling can contribute NEC susceptibility A forward genetic screen done an infant with lethal using exome sequencing identified novel stop mutation (p.Y168X) rare missense variant...
Rho GTPases play an important and versatile role in several biological processes. In this study, we identified the zebrafish ortholog of mammalian A guanine exchange factor, synectin-binding factor (Syx), determined its vivo function mouse. We found that Syx is expressed specifically vasculature these organisms. Loss-of-function studies mouse point to a specific for angiogenic sprouting developing vascular bed. Importantly, vasculogenesis angioblast differentiation steps were unaffected syx...
The mechanisms underlying many of the human disease phenotypes associated with ciliary dysfunction and abnormal centrosome amplification have yet to be fully elucidated. Here, we present for first time that SIRT2, a nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, regulates ciliogenesis amplification. Overexpression SIRT2 in renal epithelial cells appeared disrupt cilia formation, causing decreased numbers length, while inhibition activity by treatment or knockdown siRNA was...
The extracellular matrix plays a critical role in neural crest (NC) cell migration. In this study, we characterize the contribution of novel GPI-linked metalloproteinase (MMP) zebrafish mmp17b. Mmp17b is expressed post-gastrulation developing NC. Morpholino inactivation mmp17b function, or chemical inhibition MMP activity results aberrant NC migration with minimal change proliferation apoptosis. Intriguingly, GPI anchored protein inhibitor properties, Reversion-inducing-Cysteine-rich Kazal...
Mitochondria regulate intracellular calcium ion (Ca 2+ ) signaling by a fine-tuned process of mitochondrial matrix (m) Ca influx, mCa buffering (sequestration) and release efflux). This is critically important in the neurosynaptic terminal, where there simultaneous high demand for ATP utilization, cytosolic (c) regulation, maintenance ionic gradients across cell membrane. Brain synaptic non-synaptic mitochondria display marked differences retention capacity. We hypothesized that handling...
In this study, we have identified a novel member of the AMPK family, namely Sucrose non-fermenting related kinase (Snrk), that is responsible for maintaining cardiac metabolism in mammals. SNRK expressed heart, and brain, cell types such as endothelial cells, smooth muscle cells cardiomyocytes (CMs). Snrk knockout (KO) mice display enlarged hearts, die at postnatal day 0. Microarray analysis embryonic 17.5 blood profile neonates defect lipid metabolic pathways. knockdown CMs showed altered...
Tie1 (tyrosine kinase containing immunoglobulin and epidermal growth factor homology 1), an endothelial hematopoietic cell-specific receptor tyrosine kinase, is important regulator of angiogenesis critical for maintaining vascular integrity. The post-transcriptional regulation tie1 mRNA expression not understood, but it might partly explain Tie1's differential pattern in endothelium. Following up on our previous work that identified natural antisense transcripts from the locus-tie1 (tie1AS),...
A major hurdle preventing effective interventions for patients with mild traumatic brain injury (mTBI) is the lack of known mechanisms long-term cognitive impairment that follows mTBI. The closed head impact model repeated engineered rotational acceleration (rCHIMERA), a non-surgical animal mTBI (rmTBI), mimics key features rmTBI in humans. Using rCHIMERA rats, this study was designed to characterize rmTBI-induced behavioral disruption, underlying electrophysiological changes medial...
Despite their importance as members of the Roundabout (Robo) family in control axonal and vascular patterning, transcriptional regulation these genes is poorly understood. In this study, we show that Sry-related high mobility box (Sox) transcription factor being regulators roundabout4 (robo4), a Robo gene member participates sprouting angiogenesis vivo, zebrafish. Double whole mount situ hybridization analysis zebrafish embryos revealed co-localization relevant Sox factors sox7 or sox18 mRNA...
Recent evidence suggests that a majority of RNAs in the genome do not code for proteins. They are located sense (S) or antisense (AS) orientation and, to date, functional significance these non-coding (ncRNAs) is poorly understood. Here, we examined relationship between S and AS transcripts regulation key angiogenesis gene, Delta-like 4 (Dll4).Rapid Amplification cDNA Ends (RACE) method was used identify natural Dll4 gene locus murine human endothelial cells, referred as Anti-Sense...
Abstract Alginate (Alg), chitosan (Chi), collagen (Col), gelatin (Gel), and the mixtures of every two them were screened to determine their suitability for hepatocyte culture. The test materials fabricated as films then evaluated on basis abilities promote attachment functions rat hepatocytes cultured them. Cellular Col Gel was favorable. However, cellular viability, cytoskeleton organization function, by albumin production, ureagenesis, enzyme activity cytochrome P450 well expression levels...
The hepatocyte nuclear factor (HNF4alpha) has been implicated in liver development and hepatocellular differentiation. HNF4alpha expression stem cells not yet fully investigated. Here, we describe the characterization of isolated from 2-acetylaminofluorene/partial hepatectomy (AAF/PH) rat model by a combination density-gradient centrifugation selective enzymatic digestion. obtained were identified flow cytometry (FCM) immediately then immunocytochemisty reverse transcription-polymerase chain...
Coastal wetlands in Qinhuangdao play a vital role the regional ecological environment. The value of ecosystem services coastal wetland was evaluated terms provision services, regulation culture and supporting by particular methods. Results indicated that area about 39,918.00 hm2 annual 2015 around 174.32 × 108 yuan (yuan: Chinese Currency, 6.5 = 1 USD as 2015) (about $2.68 billion). accounted for 12.3%, 3.3%, 55.0% 29.4% total value, respectively. Recreation had highest proportion (54.4%),...