A5091906718- Aquaculture Nutrition and Growth
- Virus-based gene therapy research
- Aquaculture disease management and microbiota
- Reproductive biology and impacts on aquatic species
- Xenotransplantation and immune response
- Thyroid Cancer Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Physiological and biochemical adaptations
- Animal Genetics and Reproduction
- Lipid Membrane Structure and Behavior
- Viral Infectious Diseases and Gene Expression in Insects
- Ion Transport and Channel Regulation
- RNA modifications and cancer
- Cancer Research and Treatments
- Pancreatic and Hepatic Oncology Research
- CRISPR and Genetic Engineering
- Spaceflight effects on biology
- Neuroendocrine Tumor Research Advances
- Prenatal Screening and Diagnostics
- Turtle Biology and Conservation
- Regulation of Appetite and Obesity
- RNA Interference and Gene Delivery
- Pancreatic function and diabetes
- RNA Research and Splicing
Accademia di Belle Arti di Reggio Calabria
2021
Azienda Ospedaliera Universitaria Pisana
2018-2020
University of Pisa
2012-2020
University of Messina
2001-2018
Bluebird Bio (United States)
2011-2017
Bluebird Bio (France)
2014
Epix Pharmaceuticals (United States)
2010
Harvard University
1988-2004
Massachusetts General Hospital
1990-2004
GTx (United States)
1999
Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.2.12) (GAPDH) mRNA levels, protein, and enzymatic activity increase in 3T3-F442A adipocytes after exposure to physiological concentrations of insulin (Alexander, M., Curtis, G., Avruch, J., Goodman, H. (1985) J. Biol. Chem. 260, 11978-11985). In order understand the mechanism this regulation, we have isolated sequenced 5.4 kilobase pairs a 12-kilobase pair human genomic clone encoding functional GAPDH gene. The gene consists 9 exons 8 introns...
Hyperacute rejection of porcine organs by old world primate recipients is mediated through preformed antibodies against galactosyl-α-1,3-galactose (Galα-1,3-Gal) epitopes expressed on the pig cell surface. Previously, we generated inbred miniature swine with a null allele α-1,3-galactosyltransferase locus ( GGTA1 ) nuclear transfer (NT) gene-targeted fibroblasts. To expedite generation pigs, selected spontaneous mutant cells from fibroblast cultures heterozygous animals for use in another...
ABSTRACT Endogenous retroviruses of swine are a concern in the use pig-derived tissues for xenotransplantation into humans. The nucleotide sequence porcine endogenous retrovirus taken from lymphocytes miniature (PERV-MSL) has been characterized. PERV-MSL is type C 8,132 bp with greatest nucleic acid identity to gibbon ape leukemia virus and murine virus. Constitutive production RNA detected normal leukocytes multiple organs swine. copy numbers full-length PERV sequences per genome...
A previously published clinical trial demonstrated the benefit of autologous CD34+ cells transduced with a selfinactivating lentiviral vector (HPV569) containing an engineered β-globin gene (βA-T87Q-globin) in subject β thalassemia major. This has been modified to increase transduction efficacy without compromising safety. In vitro analyses indicated that changes resulted both increased titers (3 4 fold) and (2 3 fold). An vivo study which 58 β-thalassemic mice were transplanted vector- or...
Two independent cis-acting insulin response elements (IREs) in the gene encoding glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12], designated IRE-A and IRE-B, are sufficient to direct insulin-inducible expression. Using electrophoretic mobility shift assay, a 4-fold increase amount of DNA bound nuclear proteins was detected when extracts isolated from insulin-stimulated differentiated 3T3-L1 cells or liver rats refed...
A lentiviral vector encoding β-globin flanked by insulator elements has been used to treat β-thalassemia (β-Thal) successfully in one human subject. However, a clonal expansion was observed after integration the HMGA2 locus, raising question of how commonly would be associated with possible insertional activation. Here, we report correcting β-Thal murine model using same and busulfan-conditioning regimen, allowing us investigate efficacy evolution at 9.2 months transplantation bone marrow...
Successful hematopoietic cell allotransplantation results in donor-specific tolerance, but this approach has been unsuccessful the wild-type pig-to-baboon xenotransplantation model, as pig cells were lost from circulation within 5 days. However, after cessation of immunosuppressive therapy on day 28, all baboons demonstrated non-specific unresponsiveness mixed leukocyte reaction (MLR) for at least 30 We have now investigated transplantation bone marrow (BM) miniature swine homozygous...
In intensive aquaculture, basic studies on fish digestive capacity and metabolism play a key role for diet formulation.In particular, rearing of new species is strictly related to the development appropriate nutritional protocols therefore knowledge specific features concerning physiology nutrition needed.Nutrient utilization by direct function availability suitable enzymes along gastro-intestinal tract.Therefore determination enzymatic activities (proteases, carbohydrases lipases) may...
// Anello Marcello Poma 1 , Vincenzo Condello Maria Denaro Liborio Torregrossa 2 Rossella Elisei 3 Paolo Vitti and Fulvio Basolo Department of Surgical, Medical, Molecular Pathology Critical Area, University Pisa, Italy Section Pathology, Hospital Clinical Experimental Medicine, Unit Endocrinology, Correspondence to: Basolo, email: fulvio.basolo@med.unipi.it Keywords: thyroid cancer; DICER1 ; miRNA; mutation; follicular adenoma Received: December 03, 2018 Accepted:...
Salivary gland tumors (SGTs) are rare of the head and neck with different clinical behavior. Preoperative diagnosis, based on instrumental cytologic examinations, is crucial for their correct management. The identification molecular markers might improve accuracy pre-surgical diagnosis helping to plan proper treatment especially when a definitive only cytomorphology cannot be achieved. miRNAs appear new promising biomarkers in prognosis cancer. Studies concerning useful miRNA expression...
After tooth extraction, the alveolar bone tends to shrink in volume, especially on vestibular side. The role of myofibroblasts remodeling has not been sufficiently investigated. aim present study was explore gene expression related presence and activity during a 90-day healing period after extraction. included 36 rabbits, single extraction performed each rabbit. extractive sockets were randomly distributed natural or scarification wound. sacrifices staggered such manner that animals...
Abstract Junctional adhesion molecule A (JAM‐A) is a transmembrane protein that contributes to different biological process, including the epithelial mesenchymal transition (EMT). Through an EMT profiler array, we explored molecular players associated with human thyroid cancer progression and identified JAM‐A as one of genes mostly deregulated. The quantitative real‐time polymerase chain reaction immunohistochemistry analyses showed downregulation occurred in anaplastic carcinoma (ATC)...
Gastrointestinal function in vertebrates is influenced by stressors, such as fasting and refeeding, different types of diet hormonal factors. The aim this paper was to analyze the effect a Spirulina (Arthrospira platensis) diet, microalga known for its nutraceutical properties, on gastrointestinal tract zebrafish (Danio rerio) regarding expression oligopeptide transporter 1 (PepT1) ghrelin (GHR). Food deprivation refeeding investigated elucidate PepT1 GHR at level compensatory mechanism....
Increasing evidence suggests a contribution of epigenetic processes in promoting cancer and autoimmunity. Myasthenia gravis (MG) is an autoimmune disease mediated, approximately 80% the patients, by antibodies against nicotinic acetylcholine receptor (AChR+). Moreover, epithelial tumours (thymomas) are present about 10-20% there indication that changes DNA methylation might contribute to risk progression thymomas. However, role epigenetics MG still not completely clarified. In study we...
Parathyroid carcinoma (PC) is one of the rarest and aggressive malignancies endocrine system. In some instances, histological diagnosis remains uncertain unless there evidence gross local invasion or secondary spread. The identification molecular markers could improve diagnostic accuracy these lesions. expression 740 genes involved in tumor progression processes was assessed 8 parathyroid adenomas (PAs), 17 non-metastatic 10 metastatic PCs using NanoString technology. Clustering analysis...
We have investigated whether a state of tolerance toward EGFP-expressing skin tissue can be induced by prior establishment EGFP molecular chimerism transplant gene-transduced bone marrow in mice. Irradiated (10 Gy) C57BL/6J mice were transplanted with cells transduced two different retroviral vectors encoding EGFP. EGFP-transduced, mock-transduced, and age-matched control received grafts from both C57BL/6 EGFP-transgenic (B6-EGFP. Tg) MHC-mismatched B10.A donor at 8, 29, or 39 weeks after...