A5056435408- Nuclear Structure and Function
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Adipokines, Inflammation, and Metabolic Diseases
- Peroxisome Proliferator-Activated Receptors
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Titanium Alloys Microstructure and Properties
- Cardiovascular Disease and Adiposity
- RNA modifications and cancer
- Intermetallics and Advanced Alloy Properties
- Hippo pathway signaling and YAP/TAZ
- Endoplasmic Reticulum Stress and Disease
- Retinal Diseases and Treatments
- Pancreatic function and diabetes
- Advanced materials and composites
- Diabetes and associated disorders
- Cholesterol and Lipid Metabolism
- 14-3-3 protein interactions
- Histone Deacetylase Inhibitors Research
- Diet, Metabolism, and Disease
- Clusterin in disease pathology
- Protein Degradation and Inhibitors
First Affiliated Hospital of Fujian Medical University
2023-2025
Fujian Medical University
2023-2025
Augusta University
2015-2024
Nantong University
2011-2024
Affiliated Hospital of Nantong University
2024
Second Affiliated Hospital of Nantong University
2024
Jimei University
2024
Tianma Microelectronics (China)
2024
Augusta University Health
2017-2023
Ningbo First Hospital
2023
PNPLA3 (adiponutrin), a novel patatin-like phospholipase domain-containing enzyme, is expressed at high level in fat, but also other tissues including liver. Polymorphisms have been linked to obesity and insulin sensitivity. Notably, nonsynonymous variant rs738409(G) allele of the gene was found be strongly associated with both nonalcoholic alcoholic fatty liver disease. We generated Pnpla3(-/-) mice by targeting. Loss Pnpla3 has no effect on body weight or composition, adipose mass,...
Berardinelli-Seip congenital lipodystrophy 2 (BSCL2) is caused by loss-of-function mutations in SEIPIN, a protein implicated both adipogenesis and lipid droplet expansion but whose molecular function remains obscure. Here, we identify physical functional interactions between SEIPIN microsomal isoforms of glycerol-3-phosphate acyltransferase (GPAT) multiple organisms. Compared to controls, GPAT activity was elevated SEIPIN-deficient cells tissues kinetic values were altered. Increased appears...
Mutations in BSCL2 underlie human congenital generalized lipodystrophy. We inactivated Bscl2 mice to examine the mechanisms whereby absence of leads adipose tissue loss and metabolic disorders. Bscl2(-/-) develop severe lipodystrophy white (WAT), dyslipidemia, insulin resistance, hepatic steatosis. In vitro differentiation both murine embryonic fibroblasts (MEFs) stromal vascular cells (SVCs) reveals normal early-phase adipocyte but a striking failure terminal due unbridled cyclic AMP...
Docosahexaenoic acid (DHA(22:6n3)), the principal n3-polyunsaturated fatty (PUFA) in retina, has been shown to have a pronounced anti-inflammatory effect numerous vivo and vitro studies. Despite importance of vascular inflammation diabetic retinopathy, role DHA(22:6n3) cytokine-stimulated human retinal endothelial cells (hRVECs) not addressed.Cytokine-induced expression cell adhesion molecules (CAMs) was assessed by Western blot. The on cytokine-induced nuclear factor (NF)-kappaB signaling...
Abstract Mutations in the Berardinelli-Seip congenital lipodystrophy 2 gene (BSCL2) are underlying defect patients with generalized type 2. BSCL2 encodes a protein called seipin, whose function is largely unknown. In this study, we investigated role of Bscl2 regulation adipocyte differentiation. mRNA highly up-regulated during standard hormone-induced adipogenesis 3T3-L1 cells vitro. However, up-regulation does not occur mesenchymal stem cell (C3H10T1/2 cells) commitment to preadipocyte...
OBJECTIVE The results of the Diabetes Control and Complications Trial/Epidemiology Interventions cohort study revealed a strong association between dyslipidemia development diabetic retinopathy. However, there are no experimental data on retinal fatty acid metabolism in diabetes. This determined retinal-specific control animals. RESEARCH DESIGN AND METHODS Tissue gene protein expression profiles were by quantitative RT-PCR Western blot streptozotocin-induced rats at 3–6 weeks Fatty assessed...
Plin4 is a lipid droplet protein (LDP) found predominantly in white adipose tissue (WAT). The gene immediately downstream of the Plin5 gene; two genes exhibit distinct though overlapping expression patterns. absent brown (BAT) and liver expressed at low levels heart skeletal muscle, whereas highly these oxidative tissues but level WAT. physiological role remains unclear. We have generated Plin4(-/-) mice by targeting. Loss has no effect on body weight or composition mass development....
Significance Myocardium thickening at midgestation to late gestation is crucial for the formation of a functionally competent postnatal heart. However, posttranslational mechanisms regulating this process remain unexplored. Here, we uncover critical role ubiquitin-like protein NEDD8 in heart development. By targeting E1 enzyme that mediates conjugation targets, show modification essential ventricular compaction and function. We further identify substrate Cullin 7 degradation Hippo kinase...
Docosahexaenoic acid (DHA(22:6,n3)) is the principal n3 polyunsaturated fatty (PUFA) in retina. The authors previously demonstrated that DHA(22:6,n3) inhibited cytokine-induced adhesion molecule expression primary human retinal vascular endothelial (hRVE) cells, target tissue affected by diabetic retinopathy. Despite importance of inflammation retinopathy, mechanisms underlying anti-inflammatory effects cells are not understood. In this study address hypothesis acts through modifying lipid...
To investigate if intracellular glycerol content plays a role in the regulation of insulin secretion pancreatic beta cells, we studied expression channels, or aquaglyceroporins, encoded by aquaporin 3 (Aqp3), Aqp7, and Aqp9 genes mouse islets. We found Aqp7 only, not that Aqp3 Aqp9, endocrine pancreas at both mRNA (by reverse transcription-PCR) protein immunohistochemistry) levels. Immunohistochemistry revealed complete overlap between immunostaining islet. Inactivation gene targeting...
Glutaredoxins (Grxs) have been shown to be critical in maintaining redox homeostasis living cells. Recently, an emerging subgroup of Grxs with one cysteine residue the putative active motif (monothiol Grxs) has identified. However, biological and physiological functions this group proteins not well characterized. Here, we characterize a mammalian monothiol Grx (Grx3, also termed TXNL2/PICOT) high similarity yeast ScGrx3/ScGrx4. In expression assays, Grx3s were localized nuclei able rescue...
Renal osteodystrophy is commonly seen in patients with chronic kidney disease (CKD) due to disrupted mineral homeostasis. Given the impaired renal function these patients, common anti-resorptive agents, including bisphosphonates, must be used caution or even contraindicated. Therefore, an alternative therapy without burden combat urgently needed. Here, we report that clinically relevant aerobic exercise significantly prevents high-turnover CKD mouse and compromising function....
The initial determinants of retinal microvascular damage in diabetic retinopathy are not well understood, but likely to be induced by hyperglycemia and/or dyslipidemia. purpose this study was examine the effect fatty acids and on human vascular endothelial (hRVE) cells as a means mimicking metabolic disorders.The expression adhesion molecules hRVE umbilical vein (HUVECs) assayed Western blot analysis confirmed leukocyte assay. mechanisms underlying induction were further investigated using...
Research activities are currently being conducted to study multiphase flow in hydrate-bearing sediments (HBS). In this study, view of the assumption that hydrates evenly distributed HBS with two major hydrate-growth patterns, i.e., pore filling (PF hydrates), wall coating (WC hydrates) and a combination two, theoretical relative permeability model is proposed for gas-water through HBS. Besides, model, change structure (e.g., radius) due effective stress taken into account. Then, validation...
Abstract The conjugation of neural precursor cell expressed, developmentally downregulated 8 (NEDD8) to target proteins, termed neddylation, participates in many cellular processes and is aberrant various pathological diseases. Its relevance liver function failure remains poorly understood. Herein, we show dysregulated expression NAE1, a regulatory subunit the only NEDD8 E1 enzyme, human acute failure. Embryonic- adult-onset deletion NAE1 hepatocytes causes hepatocyte death, inflammation,...
Bscl2(-/-) mice recapitulate many of the major metabolic manifestations in Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2) individuals, including lipodystrophy, hepatomegly, hepatic steatosis, and insulin resistance. The mechanisms that underlie steatosis resistance are poorly understood. To address this issue, we performed hyperinsulinemic-euglycemic clamp on wild-type after an overnight (16-h) fast, found actually displayed increased sensitivity. Interestingly, liver a short term...
Mutations in BSCL2/SEIPIN cause Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2), but the mechanisms whereby Bscl2 regulates adipose tissue function are unclear. Here, we generated (mature) knockout (Ad-mKO) mice, which was specifically ablated adipocytes of adult animals, to investigate impact acquired deletion on and energy balance. Ad-mKO mice displayed reduced adiposity were protected against high fat diet-induced obesity, not insulin resistance or hepatic steatosis. Gene...
Leptin is the current treatment for metabolic disorders associated with acquired and congenital generalized lipodystrophy (CGL). Although excess leptin levels have been vascular inflammation cardiovascular disease in context of obesity, effects chronic on function remain unknown CGL. Here, we hypothesized that will improve endothelial via direct mechanisms. We investigated consequences deficiency supplementation male gBscl2-/- (Berardinelli-Seip 2 gene-deficient) mice-a mouse model CGL mice...
Mutations in the BSCL2 gene underlie human type 2 Berardinelli-Seip congenital lipodystrophy (BSCL2) disease. Global Bscl2–/– mice recapitulate and results development of insulin resistance hypertrophic cardiomyopathy. The pathological mechanisms underlying cardiomyopathy are controversial. Here we report that develop cardiac hypertrophy because increased basal IGF1 receptor–mediated (IGF1R-mediated) PI3K/AKT signaling. hearts exhibited adipose triglyceride lipase (ATGL) protein stability...
Cardiac maturation is crucial for postnatal cardiac development and increasingly known to be regulated by a series of transcription factors. However, post-translational mechanisms regulating this process remain unclear. Here we report the indispensable role neddylation in maturation. Mosaic deletion NAE1, an essential enzyme neddylation, neonatal hearts results rapid cardiomyopathy heart failure. NAE1 deficiency disrupts transverse tubule formation, inhibits physiological hypertrophy,...