A5060393718- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Diet, Metabolism, and Disease
- Diabetes and associated disorders
- Diabetes Management and Research
- Nerve injury and regeneration
- Adipose Tissue and Metabolism
- Pain Mechanisms and Treatments
- Lipoproteins and Cardiovascular Health
- Diet and metabolism studies
- Mesenchymal stem cell research
- Cholesterol and Lipid Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Adipokines, Inflammation, and Metabolic Diseases
- Neurogenesis and neuroplasticity mechanisms
- Liver Disease Diagnosis and Treatment
- Virus-based gene therapy research
- Wound Healing and Treatments
- Metabolism and Genetic Disorders
- RNA Interference and Gene Delivery
- Amyotrophic Lateral Sclerosis Research
- Botulinum Toxin and Related Neurological Disorders
Shiga University of Medical Science
2015-2024
Chubu University
2023
Shiga University
2004-2023
Baylor College of Medicine
2003-2015
Kinjo Gakuin University
2015
Showa University
2015
Fukushima Medical University
2015
Suzuki (Japan)
2015
University of Chicago
2004-2005
Texas Medical Center
2002-2003
Previous reports showed that recombinant fragments of adiponectin (adipo) displayed pharmacological effects when injected into rodents, but the relevance these observations to physiological function adipo is unclear. We generatedAdipo −/− mice by gene targeting.Adipo are fertile with normal body and fat pad weights. Plasma glucose insulin levels ofAdipo Adipo +/+mice similar under fasting conditions during an intraperitoneal tolerance test (GTT). Insulin (ITT) also produces plasma in two...
We have reported that a deficiency of tetrahydrobiopterin (BH(4)), an active cofactor endothelial NO synthase (eNOS), contributes to the dysfunction through reduced eNOS activity and increased superoxide anion (O(2)(-)) generation in insulin-resistant state. To further confirm this hypothesis, we investigated effects dietary treatment with BH(4) on endothelium-dependent arterial relaxation vascular oxidative stress aortas rats. Oral supplementation (10 mg. kg(-1). d(-1)) for 8 weeks...
Insulin-producing cells normally occur only in the pancreas and thymus. Surprisingly, we found widespread insulin mRNA protein expression different diabetic mouse rat models, including streptozotocin-treated mice rats, ob / mice, fed high-fat diets. We detected proinsulin- insulin-positive liver, adipose tissue, spleen, bone marrow, thymus; many also produced glucagon, somatostatin, pancreatic polypeptide. By situ nucleic acid hybridization, diabetic, but not nondiabetic, liver exhibited...
Tumor necrosis factor (TNF)-α is a potent proinflammatory cytokine involved in the pathogenesis of diabetic neuropathy. We inactivated TNF-α to determine if it valid therapeutic target for treatment effected inactivation neuropathy using two approaches: by genetic (TNF-α(-/-) mice) or neutralization protein monoclonal antibody infliximab. induced diabetes streptozotocin wild-type and TNF-α(-/-) mice. measured serum concentration level mRNA dorsal root ganglion (DRG) evaluated nerve function...
Immature rat intestinal stem cells (IEC-6) given the ability to express transcription factor, pancreatic duodenal homeobox 1 (Pdx-1), yielded YK cells. Although these produced multiple enteroendocrine hormones, they did not produce insulin. Exposure of 2 nmol/l betacellulin BYK that showed presence insulin expression in cytoplasm and secreted into culture media. By examining mechanism differentiation cells, we found another islet factor (Isl-1) was newly expressed with disappearance Pax-6...
Targeted disruption of the lipid droplet protein, perilipin, in mice leads to constitutional lipolysis associated with marked reduction white adipose tissue as a result unbridled lipolysis. To investigate metabolic adaptations response constitutive lipolysis, we studied perilipin-null (plin(-/-)) terms their fatty acid oxidation and glycerol glucose metabolism homeostasis by using dynamic biochemical testing clamp tracer infusion methods. plin(-/-) showed increased beta-oxidation muscle,...
To investigate if intracellular glycerol content plays a role in the regulation of insulin secretion pancreatic beta cells, we studied expression channels, or aquaglyceroporins, encoded by aquaporin 3 (Aqp3), Aqp7, and Aqp9 genes mouse islets. We found Aqp7 only, not that Aqp3 Aqp9, endocrine pancreas at both mRNA (by reverse transcription-PCR) protein immunohistochemistry) levels. Immunohistochemistry revealed complete overlap between immunostaining islet. Inactivation gene targeting...
Effects of glucagon and forskolin on the phosphorylation changes activity carnitine palmitoyltransferase (CPT) have been studied in isolated rat hepatocytes using anti-CPT immunoglobulin. When was determined lysed after or treatment, it found to be stimulated 30-80% mainly through increased affinity for palmitoyl-CoA. By SDS electrophoresis immunoprecipitates, CPT subunit (Mr 69000) noted phosphorylated 4-5-fold with (1.2 X 10(-7) M) (0.1 mM) over control. These results indicate that hepatic...
Diabetes causes skin complications, including xerosis and foot ulcers. Ulcers complicated by infections exacerbate conditions, in severe cases, limb/toe amputations are required to prevent the development of sepsis. Here, we hypothesize that hyperglycemia induces barrier dysfunction with alterations epidermal integrity. The effects on epidermis were examined streptozotocin-induced diabetic mice with/without insulin therapy. results showed dye leakages prominent, transepidermal water loss...
Neuropathic pain can be a debilitating condition. Many types of drugs that have been used to treat neuropathic only limited efficacy. Recent studies indicate pro-inflammatory mediators including tumor necrosis factor α (TNF-α) are involved in the pathogenesis pain. In present study, we engineered gene therapy strategy relieve by silencing TNF-α expression dorsal root ganglion (DRG) using lentiviral vectors expressing TNF short hairpin RNA1-4 (LV-TNF-shRNA1-4) mice. First, based on its...
A gene vector consisting of nanodiamond, polyglycerol, and basic polypeptide (ND-PG-BPP) has been designed, synthesized, characterized. The ND-PG-BPP was synthesized by PG functionalization ND through ring-opening polymerization glycidol on the surface, multistep organic transformations (-OH → -OTs (tosylate) -N3) in layer, click conjugation polypeptides (Arg8, Lys8 or His8) terminated with propargyl glycine. exhibited good dispersibility water (>1.0 mg/mL) positive zeta potential ranging...
Astrocyte- and microglia-targeting peptides were identified isolated using phage display technology. A series of procedures, including three cycles both in vivo vitro biopanning, was performed separately astrocytes M1 or M2 microglia, yielding 50–58 plaques each cell type. Analyses the sequences this collection one candidate homing peptide targeting (AS1[C-LNSSQPS-C]) two microglia (MG1[C-HHSSSAR-C] MG2[C-NTGSPYE-C]). To determine specificity for target vitro, synthesized introduced into...
To examine the effects of chronic hyperinsulinemia on vascular tissues, we examined production superoxide anion ([Formula: see text]) in aortic tissues control and exogenously hyperinsulinemic rats performed by implantation an insulin pellet for 4 wk. [Formula: text]production segments from was 2.4-fold (lucigenin chemiluminescence method) 1.7-fold (cytochrome c that without any differences text]degrading activities respectively ( P < 0.025). The increment completely abolished presence...
Gene regulation in the cardiovascular tissues of diabetic subjects has been reported to be altered. To examine abnormal activities transcription factors as a possible cause this altered gene regulation, we studied activity two redox-sensitive factors--nuclear factor-kappaB (NF-kappaB) and activating protein-1 (AP-1)--and change mRNA content heme oxygenase-1, which is regulated by these cardiac rats with streptozotocin-induced diabetes. Increased NF-kappaB AP-1 but not nuclear...
Plasma sterol levels in a family of sitosterolemia and xanthomatosis were determined by high performance liquid chromatography. Three affected siblings manifested marked including subcutaneous soft tissues generalized atherosclerosis. Two other as well children the patients did not show such clinical symptoms signs. cholesterol, sitosterol, campesterol, cholestanol three subjects 190 +/- 18.5, 25.9 11.6, 16.1 7.8, 1.84 0.92 mg/dl (mean SD), respectively. Four daughters subjects, who should...