A5009049742- Nerve injury and regeneration
- Spinal Cord Injury Research
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Reconstructive Surgery and Microvascular Techniques
- Wound Healing and Treatments
- Sarcoma Diagnosis and Treatment
- Nerve Injury and Rehabilitation
- Bone Tissue Engineering Materials
- Bone Tumor Diagnosis and Treatments
- Risk and Safety Analysis
- Urological Disorders and Treatments
- Surgical Sutures and Adhesives
- Toxoplasma gondii Research Studies
- Pluripotent Stem Cells Research
- Viral gastroenteritis research and epidemiology
- Spine and Intervertebral Disc Pathology
- Sexual function and dysfunction studies
- Neurogenetic and Muscular Disorders Research
- Veterinary Orthopedics and Neurology
- Phosphodiesterase function and regulation
- Urinary Bladder and Prostate Research
- Periodontal Regeneration and Treatments
- Protein Kinase Regulation and GTPase Signaling
Tachikawa Hospital
2012-2025
Yale University
2006-2023
Shiga University of Medical Science
2018-2022
Suzuki (Japan)
1995-2020
Matsumoto Livestock Hygiene Service Center
1989-2019
Analysis Group (United States)
2019
Konica Minolta (Japan)
2019
National Taiwan University
2019
Mitsui (Japan)
2019
Kitano Hospital
2009-2018
BRAF V600E is the most frequent oncogenic protein kinase mutation known. Furthermore, inhibitors targeting “active” kinases have demonstrated significant utility in therapeutic repertoire against cancer. Therefore, we pursued development of specific B-Raf, and allele particular. By using a structure-guided discovery approach, potent selective inhibitor active B-Raf has been discovered. PLX4720, 7-azaindole derivative that inhibits with an IC 50 13 nM, defines class marked selectivity both...
Bone marrow stromal cells (MSCs) have the capability under specific conditions of differentiating into various cell types such as osteocytes, chondrocytes, and adipocytes. Here we demonstrate a highly efficient induction with neuronal characteristics, without glial differentiation, from both rat human MSCs using gene transfection Notch intracellular domain (NICD) subsequent treatment bFGF, forskolin, ciliary neurotrophic factor. expressed markers related to neural stem after NICD, trophic...
Bone marrow stromal cells (MSCs) have the capability under specific conditions of differentiating into various cell types such as osteocytes, chondrocytes, and adipocytes. Here we demonstrate a highly efficient induction with neuronal characteristics, without glial differentiation, from both rat human MSCs using gene transfection Notch intracellular domain (NICD) subsequent treatment bFGF, forskolin, ciliary neurotrophic factor. expressed markers related to neural stem after NICD, trophic...
Abstract Transplantation of bone marrow stromal cells (MSCs) has been regarded as a potential approach for promoting nerve regeneration. In the present study, we investigated influence MSCs on spinal cord neurosphere in vitro and regeneration injured vivo by grafting. from adult rats were cocultured with fetal cord‐derived either cell mixing or making monolayered‐feeder cultures. mixed cultures, neuroshpere stimulated to develop extensive processes. numerous processes appeared be attracted...
Bone morphogenetic proteins (BMP) are unique molecules with a specific biological activity for inducing ectopic bone formation when implanted suitable carrier matrix. However, incorporation of BMP into the has disadvantages, including early burst release and protein degradation in environments. Therefore, we considered that next greatest challenge achieving successful clinical use was development system site-specific delivery signal BMP. In this study, novel BMP-2-derived oligopeptide,...
Calcium alginate dressings have beneficial effects on wound healing by providing a moist environment. However, cytotoxicity and the nonbiodegradable nature of calcium induce unresolved chronic foreign-body reaction. In this study, novel freeze-dried gel dressing (AGA-100) low in ions was evaluated for to L929 cells vitro full-thickness pig wounds vivo. Cytotoxicity testing showed cytocompatibility AGA-100 extracts, while extracts from Kaltostat, well-established dressing, induced cytopathic...
Freeze-dried alginate sponge cross-linked with covalent bonds has been demonstrated to enhance nerve regeneration in peripheral nerves and spinal cords. The present study examined, at early stages after surgery, the outgrowth of regenerating axons reactions astrocytes stump transected cord young rats. Two segments (Th7–8) were resected, was implanted lesion. As controls, collagen gel place or lesion left without implantation. 4 weeks astrocyte examined. Many axons, some which accompanied by...
Saito, Fukuki MD; Nakatani, Toshio Iwase, Masaaki Maeda, Yuji Hirakawa, Akihiko Murao, Yoshinori Suzuki, Yoshihisa Onodera, Rie MS; Fukushima, Masanori Ide, Chizuka MD Author Information
Purpose: To determine whether intrathecal administration of cultured autologous bone marrow stromal cells (BMSCs) is safe and feasible for treatment subacute spinal injury. Methods: Five patients with complete tetraplegia due to cervical in
An ideal drug delivery system (DDS) releases an appropriate at specific locations and times. We tried to create a new antibiotic that gentamicin only when wounds are infected by Pseudomonas aeruginosa (P.A.). Exudate from the dorsal pouch of rats with P.A. showed significantly higher hydrolytic activity—thrombin-like activity—toward Boc-Val-Pro-Arg-MCA than exudate noninfected wounds. therefore constructed device for controlled release antimicrobial triggered thrombin-like activity. Briefly,...
Abstract A novel synthetic peptide corresponding to BMP‐2 residues 73–92 that can induce bone formation and form a conjugate with carrier localize its effect has been reported previously. The was bound BMP‐2–specific receptor, it elevated both the alkaline phosphatase activity osteocalcin mRNA in murine multipotent mesenchymal cell line, C3H10T1/2. also induced ectopic when conjugated covalently crosslinked alginate gel implanted into rat's calf muscle. Here, is peptide‐conjugated particles...
Abstract We have developed an alginate gel crosslinked with covalent bonds for regeneration of dermis, nerve, and bone. Recently, a novel matrix (H/A gel) which consists heparin covalently ethylenediamine, was designed. It can stabilize release biologically active basic fibroblast growth factor (bFGF) 1 month, is one the heparin‐binding factors. In present report, we examined effect this H/A on nerve in rat sciatic nerve. study, regenerated axons bFGF grew faster than ordinary early stage....
The receptor tyrosine kinase KIT and its ligand stem cell factor (SCF) are required for the development of hematopoietic cells, germ other cells. A variety human cancers, such as acute myeloid leukemia, gastrointestinal stromal tumor, mast driven by somatic gain-of-function mutations. Here, we report cryo electron microscopy (cryo-EM) structural analyses full-length wild-type two oncogenic mutants, which show that overall symmetric arrangement extracellular domain ligand-occupied dimers...