A5079112300- Sarcoma Diagnosis and Treatment
- Head and Neck Cancer Studies
- Bone Tumor Diagnosis and Treatments
- Salivary Gland Tumors Diagnosis and Treatment
- Plant Virus Research Studies
- Ear and Head Tumors
- Multiple Myeloma Research and Treatments
- Oral and Maxillofacial Pathology
- Bone Metabolism and Diseases
- CRISPR and Genetic Engineering
- Cardiac tumors and thrombi
- Head and Neck Surgical Oncology
- Neurofibromatosis and Schwannoma Cases
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Cancer Diagnosis and Treatment
- Protein Degradation and Inhibitors
- Poxvirus research and outbreaks
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Bacteriophages and microbial interactions
- Bone health and treatments
- Hydraulic and Pneumatic Systems
Keio University
2010-2025
Keio University Hospital
2016-2024
Massachusetts General Hospital
2019-2021
Harvard University
2021
Kyoto University
2006-2021
Okayama University
2012-2021
Showa University
2008-2020
Showa University Koto Toyosu Hospital
2015-2020
National Cancer Center
2019-2020
Nagoya University
2020
Although it has been well established that TGF-beta plays a pivotal role in immune regulation, the roles of its downstream transcription factors, Smad2 and Smad3, have not fully clarified. Specifically, function system investigated because embryonic lethality Smad2-deficient mice. In this study, we generated T cell-specific conditional knockout (KO) mice unexpectedly found Smad3 were redundantly essential for TGF-beta-mediated induction Foxp3-expressing regulatory cells suppression IFN-gamma...
Significance Estrogen deficiency after menopause frequently accelerates osteoclastic bone resorption, leading to osteoporosis, the most common skeletal disorder in women. However, mechanisms underlying osteoporosis resulting from estrogen remain largely unknown. We report a unique mechanism postmenopausal and therapeutic target, hypoxia-inducible factor 1 alpha (HIF1α), treat this condition. HIF1α is unstable presence of oxygen, but stabilized under hypoxic conditions. we found that...
Abstract Cell–cell fusion is a dynamic phenomenon promoting cytoskeletal reorganization and phenotypic changes. To characterize factors essential for of macrophage lineage cells, we identified the multitransmembrane protein, osteoclast stimulatory transmembrane protein (OC-STAMP), analyzed its function. OC-STAMP–deficient mice exhibited complete lack cell–cell osteoclasts foreign body giant cells (FBGCs), both which are macrophage-lineage multinuclear although expression dendritic cell...
Adipocytes occupy 70% of the cellular volume within bone marrow (BM) wherein multiple myeloma (MM) originates and resides. However, nature interaction between MM cells adipocytes remains unclear. Cancer-associated support tumor through various mechanisms, including metabolic reprogramming cancer cells. We hypothesized that interactions mediate dependence on BM adipocytes. Here we show aspirates from precursor states MM, monoclonal gammopathy undetermined significance smoldering exhibit...
Abstract Osteoporosis is a complex disease with various causes, such as estrogen loss, genetics, and aging. Here we show that dominant-negative form of aldehyde dehydrogenase 2 (ALDH2) protein, ALDH2*2, which produced by single nucleotide polymorphism (rs671), promotes osteoporosis due to impaired osteoblastogenesis. Aldh2 plays role in alcohol-detoxification acetaldehyde-detoxification; however, transgenic mice expressing Aldh2*2 (Aldh2*2 Tg) exhibited severe increased levels blood...
Adequate bone curettage is crucial for treating epiphyseal chondroblastomas. However, achieving adequate while minimizing damage to the articular cartilage challenging. For lesions at center of distal femoral epiphysis, using an intercondylar approach may have better oncological and functional outcomes than conventional extra‐articular from wall epiphysis. We herein present a case 22‐year‐old male patient with chondroblastoma medial condyle close fossa. Intercondylar was performed site...
Case: Two women (aged 35 and 29 years) with intractable chronic knee infection of tumor prostheses opted for amputation. As only the trochanteric region could be preserved, we resected remaining proximal femur created a 15-cm long stump using unaffected thigh soft tissue to avoid drawbacks ultra–high-thigh amputation conventional hip disarticulation. Both had no contracture, move their voluntarily, ambulated standard transfemoral prosthesis without pelvic belt at 4-year 3-year follow-ups,...
Legius syndrome presents as an autosomal dominant condition characterized by café-au-lait macules with or without freckling and sometimes a Noonan-like appearance and/or learning difficulties. It is caused germline loss-of-function SPRED1 mutations member of the RAS-MAPK pathway syndromes. Most result in truncated protein only few inactivating missense have been reported. Since limited number patients has reported up until now, full clinical mutational spectrum still unknown. We report...
Background Leiomyosarcoma of bone (LMSoB) is a rare malignant tumor. This multicenter retrospective study was conducted to investigate the diagnosis and clinical outcome primary LMSoB in Japan. Methods Forty-eight patients (average age: 52 years [range 14–88 years]) with who were treated at registered institutes Japan between 1991 2014 recruited. The median follow-up period 44 months (range: 2–273). Results 5-year overall survival rates disease-free 78.3% 44.9%, respectively. Surgical...
Background/Aim: Despite the well-publicized clinical outcomes after unplanned excision (UE) and re-excision (re-excision) in patients with soft-tissue sarcoma (STS), there is little information about real-life referral patterns for UE, such as patient profile, details of procedures, subsequent management UE. We aimed to investigate characteristics UE who were referred sarcoma-specific centers. Patients Methods: Between May 2022 June 2023, we registered 97 underwent centers Japan. excluded...
Previously, we reported that artificial zinc-finger proteins (AZPs) inhibited virus DNA replication in planta and mammalian cells by blocking binding of a viral protein to its origin. However, the mechanisms viruses interest need be disentangled for application. To develop more widely applicable methods antiviral therapy, explored feasibility inhibition HPV-18 as model system cleaving genome. this end, fused staphylococcal nuclease monomer an AZP binds The resulting hybrid (designated...
A water-soluble Chl a/b-protein (CP673) was isolated and purified from Brussels sprouts (Brassica oleracea L. var. gemmifera DC). The protein had a molecular mass of 78 kDa an isoelectric point 4.7, consisted three or four subunits 22 extremely heat-stable. Although CP673 contained about one per protein, the blue red absorption bands that forms with different maxima suggested there are several modes sites binding for a. a/b ratio larger than 10 also indicated b is present only in small...
Recently, we demonstrated that plant DNA virus replication was inhibited in planta by using an artificial zinc finger protein (AZP) and created AZP-based transgenic plants resistant to infection. Here apply the AZP technology inhibition of a mammalian virus, human papillomavirus type 18 (HPV-18). Two AZPs, designated AZP(HPV)-1 AZP(HPV)-2, were designed our nondegenerate recognition code table constructed block binding HPV-18 E2 origin. Both newly AZPs had much higher affinities towards...
Abstract Background Myxoid liposarcoma (MLS) has the tendency to metastasize extrapulmonary. Although prognostic factors at initial diagnosis of MLS have been reported, those metastasis remain unclear. The purpose this study was investigate for disease-specific survival metastasis. Methods This retrospective observational conducted three cancer centers and two university hospitals in Japan. Of 274 patients pathologically diagnosed between 2001 2015, 48 metastatic were examined. Results Lung...
Low serum 25-hydroxyvitamin D (25(OH)D) levels are implicated as a risk factor for hip and spine fractures. Studies of the relation between 25(OH)D fractures have primarily involved elderly osteoporosis patients or with fractures; however, parathyroid hormone (PTH) status in younger adult populations remains largely unknown. We evaluated intact PTH 411 women aged 39–64 years who were not receiving medication other bone diseases. Serum positively correlated age (P = 0.019), whereas inversely...