A5000730403- Tracheal and airway disorders
- Tissue Engineering and Regenerative Medicine
- Reconstructive Surgery and Microvascular Techniques
- Electrospun Nanofibers in Biomedical Applications
- Nerve injury and regeneration
- Cleft Lip and Palate Research
- Surgical Simulation and Training
- Voice and Speech Disorders
- Soft Robotics and Applications
- Trauma Management and Diagnosis
- Nerve Injury and Rehabilitation
- Esophageal and GI Pathology
- Dysphagia Assessment and Management
- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Lung Cancer Diagnosis and Treatment
- Anatomy and Medical Technology
- Dental Implant Techniques and Outcomes
- Airway Management and Intubation Techniques
- Surgical Sutures and Adhesives
- Medical Imaging and Pathology Studies
- Augmented Reality Applications
- Spine and Intervertebral Disc Pathology
- Ear Surgery and Otitis Media
Kyoto University
2015-2024
Tokyo Medical and Dental University
2024
Kyoto University Hospital
2017-2019
Syracuse University
2017
University of Memphis
2017
Smith College
2017
Texas A&M University
2017
Louisiana State University Health Sciences Center New Orleans
2017
John Wiley & Sons (United States)
2017
Yokohama National University
2001-2016
In order to study the effects of factors governing bioabsorption rate polylactides, implantation tests were carried out in rabbits using various samples (20 x 10 2 mm3) differing their chemical composition, residual monomer content, and molecular orientation. Copolymer with a higher lactic acid content showed lower absorption, whereas those unreacted absorbed faster more drastic decrease weight. contrast, degradation purified polymers was slow; poly-L-lactide no weight change 50% reduction...
Objectives: The objective of the present study was to demonstrate regenerative medicine tracheal tissue by using an in situ engineering technique for airway reconstruction. Methods: Based on previous successful experimental animal studies, current applied repair trachea a 78-year-old woman with thyroid cancer. A Marlex mesh tube covered collagen sponge used as scaffold. operative intervention included right hemithyroidectomy, resection trachea, and tracheoplasty half three rings resected,...
Objectives: The objective of the present study was to demonstrate efficacy clinical application in situ tissue engineering using a scaffolding technique for laryngeal and tracheal tissue. Methods: We have developed scaffold made from Marlex mesh tube covered by collagen sponge. Based on successful animal experimental studies, with implant applied repair larynx trachea 4 patients. Results: In 1 patient subglottic stenosis, thyroid cartilage, cricoid cervical scarring granulation were resected...
Objective: To establish a tissue engineering therapy for the treatment of large tympanic membrane perforation (TMP) without need conventional surgical therapy. Study Design: Randomized control trial. Setting: General hospital. Patients and Methods: A total 63 chronic TMPs were randomly selected from outpatients. Intervention: Of TMPs, 53 assigned to basic fibroblast growth factor (b-FGF) group remaining 10 group. Materials used TM repair gelatin sponge fibrin glue with/without b-FGF. After...
Calcium alginate dressings have beneficial effects on wound healing by providing a moist environment. However, cytotoxicity and the nonbiodegradable nature of calcium induce unresolved chronic foreign-body reaction. In this study, novel freeze-dried gel dressing (AGA-100) low in ions was evaluated for to L929 cells vitro full-thickness pig wounds vivo. Cytotoxicity testing showed cytocompatibility AGA-100 extracts, while extracts from Kaltostat, well-established dressing, induced cytopathic...
The aim of this study was to regenerate the injured vocal fold by means selective cultured autologous mesenchymal stem cells (MSCs). Eight adult beagle dogs were used for experiment. Selective incubation MSCs from bone marrow done. These submitted 3-dimensional in 1% hydrochloric acid atelocollagen. Three-dimensional incubated injected into left fold, and atelocollagen only right same dog as a control. Four days after injection, posterior parts folds incised. regeneration estimated...
To regenerate periodontal tissues, a sandwich membrane composed of collagen sponge scaffold and gelatin microspheres containing basic fibroblast growth factor (bFGF) in controlled-release system was developed according to the new concept "in situ tissue engineering." A three-walled alveolar bone defect (3 x 4 mm) made bilaterally edentulous regions created mesially canines both maxilla mandible nine beagle dogs. with or without bFGF (100 microg) implanted each (each group, n = 18). During...
Primary pulmonary hypertension (PPH) is still a refractory disease, and patients deteriorate despite any treatment. We hypothesized that neovascularization in the lung could increase volume of vascular bed circulation thus reduce development (PH). Endothelial progenitor cells (EPCs) might be potential cell source for neovascularization. examined effects EPC transplantation into lungs dogs with dehydromonocrotaline-induced PH. The parenchyma PH model was injected ex vivo-expanded, autologous...
The feasibility of an in situ tissue-engineering method employing cell-based therapy with autologous periodontal ligament-derived cells was investigated. Periodontal ligament were obtained from six beagle dogs. fenestration defects (6 × 4 mm) created bilaterally at a location 6 mm apical to the marginal alveolar crest maxillary canines. Alkaline phosphatase-positive (3 105 cells) seeded onto collagen sponge scaffold just before implantation. One defect filled cell-scaffold construct, and...
Radiotherapy is one of the most effective treatments for head and neck cancer. However, development dry mouth syndrome an unavoidable side effect because, in addition to tumor, normal salivary glands are included irradiation field. Previously, we investigated protective efficacy basic fibroblast growth factor (bFGF) radiation-damaged glands. In this study, adipose-derived stromal cell (ADSC) transplantation regeneration radiation damaged glands.Prospective animal experiment with...
Three cDNA clones encoding putative cysteine synthases (O-acetylserine (thiol) lyase, EC 4.2.99.8) were isolated from Arabidopsis thaliana and designated AtcysC1, AtcysD1 AtcysD2, respectively. Southern blot analyses suggested that the corresponding genes present as a single copy, or at most two copies, in A. genome. Escherichia coli complementation confirmed cDNAs encode synthase proteins produced E. clearly showed activity. In addition, AtcysC1 protein β-cyanoalanine (EC 4.4.1.9) activity,...
Abstract A novel artificial nerve conduit was developed and its efficiency evaluated on the basis of promotion peripheral regeneration across an 80‐mm gap in dogs. The made a polyglycolic acid–collagen tube filled with laminin‐soaked collagen sponge. Conduits either sponge‐ or fiber‐form were implanted into peroneal (five dogs for each form). Twelve months postoperatively superior sponge group both morphometrically (percentage neural tissue: fiber: 39.7 ± 5.2, sponge: 43.0 4.5, n =3)...
This study aimed to evaluate the potential of bone marrow stromal cells for treatment inner ear diseases. Autologous labeled with Dil were implanted into five gentamicin-treated chinchillas. Histological analysis 3 weeks later revealed robust survival grafted in multiple regions within cochlea. Marrow basal turn cochlea migrated as far apical end or spiral ligament Some expressed a neuronal glial cell marker, indicating their ability differentiate cells. Survival, migrational mobility and...
In a previously reported attempt to regenerate small intestine with autologous tissues, collagen scaffolds were used without cell seeding or mesenchymal stem seeding. However the regenerated lacked smooth muscle layer. To accomplish regeneration of layer, this present study seeded cells (SMC) in canine model. Autologous SMC isolated from stomach wall and cultured. Two types fabricated: (+), cultured SMCs mixed solution poured into sponge; (−), omitted. Both implanted defects ileum as patch...