Bone morphogenetic proteins (BMP) are unique molecules with a specific biological activity for inducing ectopic bone formation when implanted suitable carrier matrix. However, incorporation of BMP into the has disadvantages, including early burst release and protein degradation in environments. Therefore, we considered that next greatest challenge achieving successful clinical use was development system site-specific delivery signal BMP. In this study, novel BMP-2-derived oligopeptide,...

Calcium alginate dressings have beneficial effects on wound healing by providing a moist environment. However, cytotoxicity and the nonbiodegradable nature of calcium induce unresolved chronic foreign-body reaction. In this study, novel freeze-dried gel dressing (AGA-100) low in ions was evaluated for to L929 cells vitro full-thickness pig wounds vivo. Cytotoxicity testing showed cytocompatibility AGA-100 extracts, while extracts from Kaltostat, well-established dressing, induced cytopathic...

An ideal drug delivery system (DDS) releases an appropriate at specific locations and times. We tried to create a new antibiotic that gentamicin only when wounds are infected by Pseudomonas aeruginosa (P.A.). Exudate from the dorsal pouch of rats with P.A. showed significantly higher hydrolytic activity—thrombin-like activity—toward Boc-Val-Pro-Arg-MCA than exudate noninfected wounds. therefore constructed device for controlled release antimicrobial triggered thrombin-like activity. Briefly,...

Alginate dressings are currently used in the management of epidermal and dermal wounds, provide a moist environment that leads to rapid granulation reepithelialization. However, cytotoxic effect on proliferation fibroblasts residual material with inflammation healing wounds have been reported recently. We developed new alginate dressing (AGA-100), which does not an inhibitory fibroblasts. The purpose this study was evaluate respect wound full- partial-thickness pig biodegradation following...

Freeze-dried alginate sponge crosslinked with covalent bonds was developed in our laboratory and has been demonstrated to enhance peripheral nerve regeneration. In this study, we examined spinal cord repair using infant rats. On postnatal day 8-12, the transversely resected at Th7-Th8 produce a 2-mm gap. The gap filled group. For control group, left empty. recovery of evoked electromyogram sensory-evoked potentials 6 weeks after surgery indicated that elongation axons could establish...

Many materials have been used for artificial tubular prostheses to assist peripheral nerve gap reconstruction. However, the clinical use of these devices has restricted because a microsurgical procedure requires specialized techniques and expensive equipment, such as operating microscope systems. Therefore authors developed new gluing method, without sutures, that uses freeze-dried alginate gel. A 7-mm in sciatic rats was bridged with Regeneration evaluated by electrophysiologic testing...

A novel material for nerve regeneration, alginate, was employed in both tubulation and nontubulation repair of a long peripheral defect injury. Twelve cats underwent severing the right sciatic to generate 50-mm gap, which treated by (n = 6) or 6). In group, conduit consisting polyglycolic acid mesh tube filled with alginate sponge implanted into gap sutured stumps. repaired simple interpolation two pieces without any suture. The animals groups exhibited similar recovery locomotor function....

Abstract Neurospheres were obtained by culturing hippocampal cells from transgenic rat fetuses (E16) expressing green fluorescent protein (GFP). The neurosphere injected into the cerebrospinal fluid (CSF) through 4th ventricle of young rats (4 weeks old) that had been given a contusion injury at T8–9 spinal cord. neural stem transported CSF to cord, attached pial surface lesion, and invaded extensively cord tissue as well nerve roots. grafted survived in host for long 8 months after...

We have recently reported that freeze-dried alginate gel, which was developed in our laboratory, enhanced peripheral nerve regeneration. The purpose of this study to examine whether gel is capable promoting regeneration the severed spinal cord adult mammals. Using Wistar rats at 30 days age (P30), T9-T10 totally resected and implanted across gap. Forty-five after surgery myelinated unmyelinated axons regenerated throughout gap with remaining gel. elongated established electrophysiologically...

A free radical gas, nitric oxide, has many useful functions when produced under physiological conditions by neurons and endothelial cells. However, excess oxide been reported to exert cytotoxic effects direct toxicity or reaction with superoxide. The effect of on the microcirculation in periphery a flap remains unclear, its survival is also unknown because dual action. Thus, we attempted clarify rat random pattern skin flaps use an constitutive synthase inhibitor (IP administration 50 mg/kg...

During the final period of embryogenesis, a funnel-shaped tube continues medially into mesenchymal tissue forming curved path. Although this may sound simple, development occurring during early fetal life is in fact very complex. At first, ectodermal cells proliferate to fill lumen meatus, meatal plug, and then at 10 weeks bottom plug extends disc-like fashion, so that horizontal plane meatus boot-shaped with narrow neck sole spreading widely form future tympanic membrane medially. same...

Object. Facial nerve paralysis due to a surgical procedure or trauma is frequently observed complication. The authors evaluated facial repair achieved by the interposition of collagen guide. Methods. Ten cats were divided into three groups. Group 1 consisted six animals in which 5-mm segment on one side was resected and replaced tube that sutured bridge both stumps. On opposite resected, reversed 180°, stumps as an autograft nerve. 2 two guide interposed other left intact. 3 placed...

An infant with congenital absence of the nostrils, incomplete prolabium, premaxilla, nasal cavity without septum, and cleft palate is presented. To clarify embryological development nostril, serial sections many embryos fetuses various stages were examined. Recanalization resulting from resorption temporary epithelial plug, was observed in a 13- to 15-week fetus. Although textbooks human embryology describe its occurrence 6th month or 24th week fetal life. This finding suggests that cause...