A5070377660- Pancreatic and Hepatic Oncology Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Mesenchymal stem cell research
- Organ Transplantation Techniques and Outcomes
- Retinal Imaging and Analysis
- Intraocular Surgery and Lenses
- Liver Disease and Transplantation
- Retinal and Macular Surgery
- Traumatic Ocular and Foreign Body Injuries
- Pediatric Hepatobiliary Diseases and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Neuroendocrine Tumor Research Advances
- Traumatic Brain Injury and Neurovascular Disturbances
- Ophthalmology and Eye Disorders
- Neurogenesis and neuroplasticity mechanisms
- Retinal and Optic Conditions
- Ocular Diseases and Behçet’s Syndrome
- Pluripotent Stem Cells Research
- Cancer Genomics and Diagnostics
- Mitochondrial Function and Pathology
- Drug-Induced Ocular Toxicity
- Multiple and Secondary Primary Cancers
- Colorectal and Anal Carcinomas
Hyogo University
2024-2025
Japan Clinical Cancer Research Organization
2017-2024
Hyogo Medical University
2013-2023
Mirai Hospital
2022-2023
Yao Tokushukai General Hospital
2021-2023
University of Miyazaki
2022
Kobe University
2022
University Medical Center Utrecht
2022
Kitasato University
2022
University of Miyazaki Hospital
2022
Bone marrow stromal cells (MSCs) have the capability under specific conditions of differentiating into various cell types such as osteocytes, chondrocytes, and adipocytes. Here we demonstrate a highly efficient induction with neuronal characteristics, without glial differentiation, from both rat human MSCs using gene transfection Notch intracellular domain (NICD) subsequent treatment bFGF, forskolin, ciliary neurotrophic factor. expressed markers related to neural stem after NICD, trophic...
Bone marrow stromal cells (MSCs) have great potential as therapeutic agents. We report a method for inducing skeletal muscle lineage from human and rat general adherent MSCs with an efficiency of 89%. Induced differentiated into fibers upon transplantation degenerated muscles rats mdx-nude mice. The induced population contained Pax7-positive that contributed to subsequent regeneration repetitive damage without additional cells. These represent more ready supply myogenic than do the rare stem...
Bone marrow stromal cells (MSCs) have the capability under specific conditions of differentiating into various cell types such as osteocytes, chondrocytes, and adipocytes. Here we demonstrate a highly efficient induction with neuronal characteristics, without glial differentiation, from both rat human MSCs using gene transfection Notch intracellular domain (NICD) subsequent treatment bFGF, forskolin, ciliary neurotrophic factor. expressed markers related to neural stem after NICD, trophic...
The long-term consequences of traumatic brain injury (TBI), specifically the detrimental effects inflammation on neurogenic niches, are not very well understood. In present in vivo study, we examined prolonged pathological outcomes experimental TBI different parts rat with special emphasis and neurogenesis. Sixty days after moderate controlled cortical impact injury, adult Sprague-Dawley male rats were euthanized tissues harvested. Antibodies against activated microglial marker, OX6, cell...
Traumatic brain injury (TBI) survivors exhibit motor and cognitive symptoms from the primary that can become aggravated over time because of secondary cell death. In present in vivo study, we examined beneficial effects human adipose-derived stem cells (hADSCs) a controlled cortical impact model mild TBI using young (6 months) aged (20 F344 rats. Animals were transplanted intravenously with 4 × 10 6 hADSCs (Tx), conditioned media (CM), or vehicle (unconditioned media) at 3 h after TBI....
Traumatic brain injury (TBI) is associated with neuro-inflammation, debilitating sensory-motor deficits, and learning memory impairments. Cell-based therapies are currently being investigated in treating neurotrauma due to their ability secrete neurotrophic factors anti-inflammatory cytokines that can regulate the hostile milieu chronic neuroinflammation found TBI. In tandem, stimulation mobilization of endogenous stem/progenitor cells from bone marrow through granulocyte colony stimulating...
Background Comprehensive stroke studies reveal diaschisis, a loss of function due to pathological deficits in brain areas remote from initial ischemic lesion. However, blood-brain barrier (BBB) competence subacute diaschisis is uncertain. The present study investigated focal rat model. Specific focuses were BBB integrity and related pathogenic processes contralateral areas. Methodology/Principal Findings In ipsilateral hemisphere 7 days after transient middle cerebral artery occlusion...
Here, we report that a unique mechanism of action exerted by stem cells in the repair traumatically injured brain involves their ability to harness biobridge between neurogenic niche and site. This biobridge, visualized immunohistochemically laser captured, corresponded an area subventricular zone cortex. That expressed high levels extracellular matrix metalloproteinases characterized initially stream transplanted cells, but subsequently contained only few non-detectable grafts overgrown...
Despite the reported functional recovery in transplanted stroke models and patients, mechanism of action underlying stem cell therapy remains not well understood. Here, we examined role cell-mediated vascular repair stroke.Adult rats were exposed to transient occlusion middle cerebral artery 3 hours later randomly stereotaxically transplantated with 100K, 200K, or 400K human endothelial 6 viable cells vehicle. Animals underwent neurological examination motor test up day 7 after...
Modified mesenchymal stromal cells (MSCs) display a unique mechanism of action during the repair phase traumatic brain injury by exhibiting ability to build biobridge between neurogenic niche and site injury. Immunohistochemistry laser capture assay have visualized this in area subventricular zone injured cortex. This expresses high levels extracellular matrixmetalloproteinases (MMPs), which are initially co-localized with stream transplanted MSCs, but later region contains only few...
Background and Purpose— Ischemic stroke is a leading cause of mortality morbidity in the world may be associated with cardiac myocyte vulnerability. However, it remains uncertain how an ischemic brain contributes to alternations. Here, we used experimental models reveal pathological effects on heart. Methods— For vitro study, primary rat neuronal cells were subjected 90-minute oxygen–glucose deprivation (OGD). Two hours after OGD, supernatant was collected cryopreserved until further...
To determine the current clinical preferences of anti-vascular endothelial growth factor (VEGF) treatment protocols for diabetic macular edema (DME) in Japan.This was a descriptive cross-sectional study. Answers to questionnaire consisting 16 questions were obtained from 176 278 (63.3%) surveyed ophthalmologists.The results showed that 81.2% preferred intravitreal injections anti-VEGF antibodies as first-line therapy. The most important indicators beginning therapy were: best-corrected...
Long-term consequences of traumatic brain injury (TBI) are closely associated with the development severe psychiatric disorders, such as post-traumatic stress disorder (PTSD), yet preclinical studies on pathological changes after combined TBI PTSD lacking. In present in vivo study, we assessed chronic neuroinflammation, neuronal cell loss, proliferation and differentiation specific regions adult Sprague-Dawley male rats following controlled cortical impact model moderate or without exposure...
We investigated the rates of use steroids in Japanese central serous chorioretinopathy (CSC) cases and differences characteristics CSC with without steroids. A total 538 eyes 477 patients diagnosed CSC, 3 months or more follow-up between April 2013 June 2017 at 8 institutions. Patients than were identified by OCT fluorescein angiography Data collected included patient demographics, history corticosteroid medication smoking, spherical errors, findings angiography, subfoveal choroidal...
Abstract Purpose To evaluate real‐world evidence for young Japanese patients with rhegmatogenous retinal detachment ( RRD ) by retrospectively examining surgical procedures and clinical outcomes in the Japan Clinical Retina Study group. Methods This was a multicentre retrospective study of medical records 562 (384 males 178 females; mean age: 33.0 ± 11.8 years) who had been diagnosed underwent participating centres during period between April 2013 August 2016. Results The selected surgeries...
Stem cells exert therapeutic effects against ischemic stroke via transplantation of exogenous stem or stimulation endogenous within the neurogenic niches subventricular zone and subgranular zone, recruited from bone marrow through peripheral circulation. In this paper, we review different sources that have been tested in animal models stroke. addition, discuss specific mechanisms action, particular neurovascular repair by endothelial progenitor cells, as key translational research for...
Stem cell-based therapies for stroke have expanded substantially over the last decade. The diversity of embryonic and adult tissue sources provides researchers with ability to harvest an ample supply stem cells. However, optimal conditions cell use are still being determined. Along this line need optimization studies, we discuss studies that demonstrate effective dose, timing, route We recognize derivations also provide uniquely individual difficulties limitations in their therapeutic...
Abstract Background Leber hereditary optic neuropathy (LHON) is an acute or subacute that mainly affects young males. The first nationwide epidemiological survey of LHON was conducted in 2014 Japan, and officially designated as a rare intractable disease by the Japanese government 2015. We second annual incidence 2019, estimated total number patients with Japan. Results A questionnaire sent to 997 facilities accredited Ophthalmological Society and/or affiliated councilors Neuro-Ophthalmology...
This retrospective observational multicentre cohort study compared the rate of postoperative cystoid macular oedema (CME) between two intraocular lens (IOL) scleral fixation (SFIOL) techniques: a flanged IOL technique (Yamane technique) and suture transscleral (conventional technique). The included 207 eyes with CME that had undergone SFIOL were observed for > 12 weeks January 2019 2021. primary endpoint was comparison at 3 months groups. Secondary endpoints best-corrected visual acuity...
Abstract In a transgenic mouse line hK14mIL33tg, with the expression of interleukin-33 (IL-33) driven by keratin 14 promoter, keratoconjunctivitis developed spontaneously between 18 and 22 weeks age under specific-pathogen-free conditions. These mice showed blepharitis corneal impairments, histology revealed epithelial thickening in conjunctiva cornea infiltration eosinophils, mast cells basophils. IL-5, IL-13 CCL11 were abundant lacrimal fluid mice, gene expressions IL-4, IL-13, IL-33, Prg2...