A5007812621- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes Treatment and Management
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cardiovascular Health and Disease Prevention
- Metabolism, Diabetes, and Cancer
- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Pancreatic function and diabetes
- Diabetes Management and Research
- Diet, Metabolism, and Disease
- Lipoproteins and Cardiovascular Health
- Liver Disease Diagnosis and Treatment
- Fatty Acid Research and Health
- Chronic Kidney Disease and Diabetes
- Peripheral Artery Disease Management
- Advanced Glycation End Products research
- Peroxisome Proliferator-Activated Receptors
- Diabetes and associated disorders
- Cholesterol and Lipid Metabolism
- Protein Tyrosine Phosphatases
- Galectins and Cancer Biology
- Regulation of Appetite and Obesity
- Antiplatelet Therapy and Cardiovascular Diseases
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
Kagoshima University
2016-2025
National Institute of Fitness and Sports in Kanoya
2024
Kobe Pharmaceutical University
2024
Kagoshima Medical Center
2023
National Hospital Organization
2023
Ajinomoto (Japan)
2019
Kumamoto University
2017
Morpho (United States)
2017
Shiga University of Medical Science
2004-2014
Kagoshima University Hospital
2012
In 1999, the Japan Diabetes Society (JDS) launched previous version of diagnostic criteria diabetes mellitus, in which JDS took initiative adopting glycated hemoglobin (HbA1c) as an adjunct to diagnosis diabetes. contrast, 2009 International Expert Committee composed members American Association (ADA) and European for Study (EASD) manifested recommendation regarding use HbA1c diagnosing mellitus alternative glucose measurements based on updated evidence showing that has several advantages a...
To investigate underlying mechanisms responsible for the impaired nitric oxide (NO)-dependent vascular relaxation in insulin-resistant state, we examined production of both NO and superoxide anion radical (O2-) those modulating factors aortas obtained from normal (CTR), insulin-treated (INS), or high fructose-fed (FR) rats. FR rats showed insulin resistance with endogenous hyperinsulinemia, whereas INS sensitivity. Only aortic strips endothelium elicited response to either acetylcholine...
We have reported that a deficiency of tetrahydrobiopterin (BH(4)), an active cofactor endothelial NO synthase (eNOS), contributes to the dysfunction through reduced eNOS activity and increased superoxide anion (O(2)(-)) generation in insulin-resistant state. To further confirm this hypothesis, we investigated effects dietary treatment with BH(4) on endothelium-dependent arterial relaxation vascular oxidative stress aortas rats. Oral supplementation (10 mg. kg(-1). d(-1)) for 8 weeks...
Excess production of superoxide anion in response to angiotensin II plays a central role the transduction signal molecules and regulation vascular tone. We examined ability insulin resistance stimulate investigated identity oxidases responsible for its production. Rats were fed diets containing 60% fructose (fructose-fed rats) or starch (control 8 weeks. In aortic homogenates from fructose-fed rats, generated NAD(P)H was more than 2-fold higher that control rats. Pretreatment aorta rats with...
To elucidate molecular mechanisms of high fructose-induced metabolic derangements and the influence peroxisome proliferator-activated receptor-α (PPARα) activation on them, we examined expression sterol regulatory element binding protein-1 (SREBP-1) PPARα as well its nuclear target gene expressions in liver fructose-fed rats with or without treatment fenofibrate. After 8-wk feeding a diet fructose, mRNA contents protein activity fatty acid oxidation enzymes were reduced. In contrast, SREBP-1...
OBJECTIVE—To document an association between arterial wall stiffness and reduced flow volume in the lower-extremity arteries of diabetic patients. RESEARCH DESIGN AND METHODS—We recruited 60 consecutive type 2 patients who had no history or symptoms peripheral disease (PAD) lower extremities normal ankle/brachial systolic blood pressure index at time study (non-PAD group) 20 age-matched nondiabetic subjects (control group). We used automatic device to measure pulse wave velocity (PWV) as...
Immature rat intestinal stem cells (IEC-6) given the ability to express transcription factor, pancreatic duodenal homeobox 1 (Pdx-1), yielded YK cells. Although these produced multiple enteroendocrine hormones, they did not produce insulin. Exposure of 2 nmol/l betacellulin BYK that showed presence insulin expression in cytoplasm and secreted into culture media. By examining mechanism differentiation cells, we found another islet factor (Isl-1) was newly expressed with disappearance Pax-6...
Insulin signaling is regulated by tyrosine phosphorylation of the molecules, such as insulin receptor and substrates (IRSs). Therefore, balance between protein-tyrosine kinases phosphatase activities thought to be important in modulation insulin-resistant states. We thus employed adenovirus-mediated gene transfer technique, we analyzed effect overexpression a wild-type phosphatase-1B (PTP1B) on both L6 myocytes Fao cells. In cells, PTP1B blocked insulin-stimulated IRS-1 more than 70%...
To assess whether an association between sodium-sensitive hypertension and metabolic syndrome exists; whether, in patients with syndrome, the nocturnal fall of blood pressure decreases salt restriction affects circadian rhythm.Japanese essential hypertension, who were treated without any antihypertensive agent, maintained on a high-sodium diet low-sodium for 1 week each. On last day each diet, 24-h pressures measured. A diagnosis was made according to International Diabetes Foundation...
Short-QT syndrome (SQTS) is a recently recognized disorder associated with atrial fibrillation (AF) and sudden death due to ventricular arrhythmias. Mutations in several ion channel genes have been linked SQTS; however, the mechanism remains unclear. This study describes novel heterozygous gain-of-function mutation inward rectifier potassium gene, KCNJ2, identified SQTS. We studied an 8-year-old girl markedly short-QT interval (QT = 172 ms, QTc 194 ms) who suffered from paroxysmal AF....
Recent studies have proposed that n-3 polyunsaturated fatty acids (n-3 PUFAs) direct antioxidant and anti-inflammatory effects in vascular tissue, explaining their cardioprotective effects. However, the molecular mechanisms are not yet fully understood. We tested whether PUFAs showed activity through activation of nuclear factor erythroid 2-related 2 (Nrf2), a master transcriptional for genes. C57BL/6 or Nrf2−/− mice were fed fish-oil diet 3 weeks. Fish-oil significantly increased expression...
A fiber-rich diet has a cardioprotective effect, but the mechanism for this remains unclear. We hypothesized that with brown rice improves endothelial function in patients type 2 diabetes mellitus.Twenty-eight mellitus at single general hospital Japan were randomly assigned to (n = 14) or white and followed 8 weeks. The primary outcome was changes determined from flow debt repayment by reactive hyperemia using strain-gauge plethysmography fasting state. Secondary outcomes HbA1c, postprandial...
The effects of glucose concentration on D-glucose oxidation and reduced nicotinamide adenine dinucleotide phosphate (NADPH) supply were studied during exposure cultured human umbilical vein endothelial cells to hydrogen peroxide (H2O2). activation via the pentose pathway (PPP), induced by 200 μmol/l H2O2 for 1 h, was 50% (P < 0.01) in 5–7 days 33 mmol/l (HG) versus those 5.5 without (NG) or with (HR) 27.5 D-raffinose. intracellular NADPH content HG cells, but not NG HR decreased 42%...
Loss of capillary pericytes, a characteristic finding in diabetic retinopathy, is strongly associated with hyperglycemia. The pathologic aberrations retinopathy are localized primarily the retinal capillaries and only poorly reversed by subsequent euglycemic control. Since hyperglycemia significantly inhibits pericyte growth culture, we investigated regulation gene expression pericytes exposed to physiologic (5.5mM) (20 mM) glucose concentrations. By utilizing modifications mRNA differential...
Cross-sectional studies have reported increased levels of urinary type IV collagen in diabetic patients with progression nephropathy. The aim this study was to determine the role predicting development and early nephropathy deterioration renal function a longitudinal study.Japanese 2 diabetes (n = 254, 185 normoalbuminuria 69 microalbuminuria) were enrolled an observational follow-up study. associations annual decline estimated glomerular filtration rate (eGFR) evaluated.At baseline, higher...
Smoking is an overwhelming, but preventable, risk factor for cardiovascular diseases (CVD), although smoking prevalence remains high in developed and developing countries East Asia.In a population-based sample of 1019 Japanese men aged 40 to 79 years, without CVD, we examined cross-sectional associations status, cumulative pack-years, daily consumption, time since cessation, with subclinical atherosclerosis at 4 anatomically distinct vascular beds, including coronary artery calcification,...