A5102790319- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Renal Diseases and Glomerulopathies
- Monoclonal and Polyclonal Antibodies Research
- Nitric Oxide and Endothelin Effects
- Chronic Kidney Disease and Diabetes
- Renal Transplantation Outcomes and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Renal and Vascular Pathologies
- Renin-Angiotensin System Studies
- Sarcoma Diagnosis and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Diabetes and associated disorders
- Angiogenesis and VEGF in Cancer
- Chronic Lymphocytic Leukemia Research
- Ion Transport and Channel Regulation
- Acute Kidney Injury Research
- Pancreatic function and diabetes
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Eicosanoids and Hypertension Pharmacology
- Vasculitis and related conditions
- Complement system in diseases
- Cardiovascular Health and Disease Prevention
- Neonatal Health and Biochemistry
University of Florida
2008-2020
North Florida/South Georgia Veterans Health System
2005-2015
Dalian Medical University
2015
Florida College
1988-2013
Malcom Randall VA Medical Center
2003-2012
The University of Texas Southwestern Medical Center
2000-2005
Florida Southern College
2004-2005
Washington University in St. Louis
2005
University of Colorado Health
2005
University of Florida Health Science Center
2005
Standardization of renal allograft biopsy interpretation is necessary to guide therapy and establish an objective end point for clinical trials. This manuscript describes a classification, Banff 97, developed by investigators using the Schema Collaborative Clinical Trials in Transplantation (CCTT) modification diagnosis pathology.Banff 97 grew from international consensus discussion begun at continued via Internet. schema (a) analysis data (b) publication experience with CCTT modification,...
Systemic lupus erythematosus is associated with enhanced CD4 + T cell metabolism and can be reversed by metabolic modulators.
We previously produced three congenic strains carrying lupus susceptibility genes ( Sle1-Sle3 ) from the lupus-prone NZM2410 mouse on C57BL/6 background and characterized their component phenotypes. Sle1 mediates loss of tolerance to nuclear antigens; Sle2 lowers activation threshold B cells; Sle3 a dysregulation CD4 + T cells. have now created collection bi- tricongenic with these intervals assessed autoimmune phenotypes they elicit in various combinations. Our results indicate that is key...
Ca<sup>2+</sup> channels in distinct subcellular compartments of neurons mediate voltage-dependent influx, which integrates synaptic responses, regulates gene expression, and initiates transmission. Antibodies that specifically recognize the α<sub>1</sub> subunits class A, B, C, D, E have been used to investigate localization these voltage-gated ion on spinal motor neurons, interneurons, nerve terminals adult rat. Class A P/Q-type were present mainly a punctate pattern located along cell...
Gut microbiota dysbiosis induces lupus-like autoimmunity in mice through altered tryptophan catabolism.
The major murine systemic lupus erythematosus (SLE) susceptibility locus Sle1 is syntenic to a chromosomal region linked with SLE in multiple human studies. Congenic analyses have shown that breaks tolerance chromatin, necessary step for full disease induction can be suppressed by specific modifier loci. In the present study, our fine mapping analysis of location has determined three loci within this congenic interval, termed Sle1a , Sle1b and Sle1c independently cause loss chromatin. Each...
Abstract We describe the in vivo phenotypes associated with three genomic intervals containing systemic lupus erythematosus (SLE)-susceptibility genes derived from SLE-prone NZM2410 strain on a C57BL/6 genome. These were identified previously via genome-wide analysis of SLE susceptibility (NZM2410 x C57BL/6)F1 backcross, and transferred independently background to produce congenic strains: B6.NZMc1 carrying Sle1, B6.NZMc4 Sle2, B6.NZMc7 Sle3. develops high titers IgG anti-nuclear...
We have previously shown that CD4(+) T cells from B6.Sle1Sle2.Sle3 lupus mice and patients present a high cellular metabolism, treatment combining 2-deoxy-D-glucose, which inhibits glucose metformin, oxygen consumption, normalized cell functions in vitro reverted disease mice. obtained similar results with B6.lpr mice, another model of lupus, showed continuous is required to maintain the beneficial effect metabolic inhibitors. Further, we investigated relative roles oxidation pyruvate...
Sle1 and Sle3 are 2 loci that confer susceptibility to lupus nephritis in the NZM2410 strain of mice. Our previous work has shown B6.NZMc1 mice, congenic for Sle1, exhibit loss tolerance chromatin but do not develop any pathogenic autoantibodies or disease. B6.NZMc7 Sle3, low-grade polyclonal B- T-cell activation, elevated CD4/CD8 ratios, mildly penetrant glomerulonephritis. In contrast these monocongenics, present study reveals B6.NZMc1|c7 bicongenic splenomegaly, significantly expanded...
Heme oxygenase-1 (HO-1) is induced as an adaptive and protective response to tissue injury. HO-1 degrades heme into carbon monoxide (CO) biliverdin; the latter then converted bilirubin. These reaction products have powerful antiapoptotic antioxidant effects. Manipulation of system by administration micromolar doses exogenous CO or bilirubin has been performed in several organ systems, but dose-related effects these not investigated kidney. The purpose this study was evaluate efficacy 1 10...
Lupus nephritis (LN) is a major contributor to morbidity and mortality in patients with systemic lupus erythematosus (SLE). There evidence that polymorphisms the genes of inflammatory mediators may predispose development LN SLE. In this study, we examined role functional monocyte chemoattractant protein 1 (MCP-1) polymorphism SLE LN.DNA paired urine serum samples were obtained from 134 (> or =4 American College Rheumatology criteria for SLE; 49 85 without LN) 118 controls. MCP-1 genomic...
Marked hyperuricemia is known to cause acute renal failure via intrarenal crystal deposition. However, recent studies suggest mild may have vasoactive and proinflammatory effects independent of formation. We therefore tested the hypothesis that might exacerbate injury dysfunction in a model cisplatin-induced rat. Cisplatin was administered normouricemic hyperuricemic rats (the latter generated by administering urate oxidase inhibitor, oxonic acid). Recombinant (rasburicase) third group...
Summary Forty-one cases of vulvar vestibulitis syndrome are presented. All were studied to provide a histopathological description this condition in terms location, intensity, and characterization the inflammatory response. Alterations normal histology minor vestibular glands forming clefts described. The association with other disease processes, special studies done elucidate an etiology, discussed.
Interleukin 10 (IL-10) is a pleiotropic cytokine with well known antiinflammatory, immunosuppressive, and immunostimulatory properties. Chronic allograft rejection, characterized by vascular neointimal proliferation, major cause of organ transplant loss, particularly in heart kidney recipients. In Dark Agouti to Lewis rat model aortic transplantation, we evaluated the effects single intramuscular injection recombinant adeno-associated viral vector (serotype 1) encoding IL-10 (rAAV1-IL-10) on...
The vascular endothelium expresses endothelial nitric oxide synthase (eNOS) that generates (NO) to help maintain integrity due its anti-inflammatory, antiproliferative, and antithrombogenic effects. Pharmacological blockade of NO production has been shown exacerbate renal injury in chronic disease induces cell loss. However, pharmacological inhibition nonspecifically blocks other types NOS therefore does not define the specific role eNOS kidney disease. We hypothesized a lack can induce loss...
Hypokalemic nephropathy is associated with alterations in intrarenal vasoactive substances, leading to vasoconstriction, salt-sensitivity, and progression of interstitial fibrosis. In this study, we investigated whether hypokalemic might also involve impaired renal angiogenesis. Sprague-Dawley rats that were fed low-potassium diets developed peritubular capillary loss began the inner stripe outer medulla (week 2) progressed 4) cortex 12). These changes increased macrophage infiltration,...
Systemic lupus erythematosus (SLE) is an autoimmune disorder that affects women more frequently than men. In the (NZB times NZW)F1 mouse, a murine SLE model, presence or absence of estrogen markedly influences rate progression disease. Three organochlorine pesticides with estrogenic effects were administered chronically to ovariectomized female mice, and we measured time development renal disease, principal clinical manifestation in this model. Treatment chlordecone, methoxychlor, o,p...
Recent studies have identified the presence of a novel Mep/Amt/Rh glycoprotein family proteins that may play an important role in transmembrane ammonia transport. One mammalian members this family, Rh C (RhCG), transports ammonia, is expressed distal nephron sites are critically for secretion, exhibits increased expression response to chronic metabolic acidosis, and originally was cloned as tumor-related protein. The purpose our determine localization RhCG normal neoplastic human kidney....