A5073201628- HIV Research and Treatment
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- HIV/AIDS Research and Interventions
- Biosimilars and Bioanalytical Methods
- HIV-related health complications and treatments
- Advanced Biosensing Techniques and Applications
- Immunodeficiency and Autoimmune Disorders
- Systemic Lupus Erythematosus Research
- HIV/AIDS drug development and treatment
- Liver Disease Diagnosis and Treatment
- Protein purification and stability
- Endoplasmic Reticulum Stress and Disease
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Autophagy in Disease and Therapy
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Lipid metabolism and biosynthesis
- Herpesvirus Infections and Treatments
- Mast cells and histamine
Amgen (United States)
2011-2024
Oregon Health & Science University
2008
University Health Network
2008
London Health Sciences Centre
2008
Amgen (Germany)
2008
Centre International De Reference Chantal Biya
2008
Hôtel-Dieu de Paris
2007
BD Biosciences (United States)
2007
UCLA Medical Center
2002
Black AIDS Institute
2000
Journal Article Cd8+ Lymphocyte Activation At Human Immunodeficiency Virus Type 1 Seroconversion: Development Of Hla-Dr+ Cd38- Cells Is Associated With Subsequent Stable Cd4+ Cell Levels Get access Janis V. Giorgi, Giorgi Departments of Medicine and Biomathematics, University California, Los Angeles (UCLA), Jonsson Comprehensive Cancer Center, UCLA School Public Health, Department BiostatisticsLos AngelesDepartments Epidemiology Environmental Health Sciences, Johns Hopkins Hygiene...
Nonalcoholic fatty liver disease, recently renamed metabolic dysfunction-associated steatotic disease (MASLD), is a progressive disorder that begins with aberrant triglyceride accumulation in the and can lead to cirrhosis cancer. A common variant gene
Journal Article Virus Burden in Long-Term Survivors of Human Immunodeficiency (HIV) Infection Is a Determinant Anti-HIV CD8+ Lymphocyte Activity Get access John Ferbas, Ferbas Departments Medicine and Microbiology Immunology, School Medicine, Department Epidemiology, Public Health, University California at Los Angeles; Therion Biologics Corporation, Cambridge, Massachusetts Search for other works by this author on: Oxford Academic PubMed Google Scholar Andrew H. Kaplan, Kaplan Mary Ann...
Abstract We determined the relative abilities of cell subpopulations from all major PBMC lineages normal donors to produce IFN-alpha in response vitro stimulation with lymphocytotropic HIV-1 (IIIb and RF), monocytotropic (BaL), Sendai virus, HSV-1. Active inactive cell-free preparations IIIb cell-associated IIIb, active other viruses, induced comparable, maximal levels acid-stable by 18 24 h. Negative selection enrichment experiments indicated that HLA-DR+ "null" cells produced majority...
To evaluate the safety, tolerability, pharmacokinetics (PK) and pharmacodynamics (PD) of single-dose multiple-dose administration AMG 557, a human anti-inducible T cell co-stimulator ligand (ICOSL) monoclonal antibody, in subjects with systemic lupus erythematosus (SLE). Patients mild, stable SLE (n=112) were enrolled two clinical trials to effects single (1.8-210 mg subcutaneous or 18 intravenous) multiple (6 -210 every other week (Q2W)×7) doses 557. Subjects received 1 intradermal...
Abstract A basic immunophenotyping panel that employed dual‐color combinations of fluorescein isothiocyanate (FITC) and phycoerythrin (PE) conjugated monoclonal antibodies (mAb; FITC‐CD45/PE‐CD14, FITC‐IgG 1 /PE‐IgG 2 , FITC‐CD3/PE‐CD8, FITC‐CD3/PE‐CD4, FITC‐CD3/PE‐CD16+PE‐CD56, PE‐CD19) was utilized in a quality assurance program to determine whether the 4 laboratories participating multicenter AIDS study obtained similar lymphocyte subset percentage values for T cells, B NK CD4 + CD8...
From May 1998 to November 2000, 13 European patients developed antibody-mediated pure red cell aplasia during treatment with recombinant human erythropoietin (rHuEPO), reinforcing the need for analytical testing antibodies against erythropoietic agents. Specimens from 8 were provided further antibody and characterization.We evaluated 4 methods these sera: radioimmune precipitation (RIP), enzyme-linked immunosorbent assay (ELISA), biosensor immunoassay, a bioassay identification of...
Abstract Flow Cytometry has become a mainstay technique for measuring fluorescent and physical attributes of single cells in suspended mixture. These data are reduced during analysis using manual or semiautomated process gating. Despite the need to gate traditional analyses, it is well recognized that analyst‐to‐analyst variability can impact dataset. Moreover, interest be inadvertently excluded from gate, relationships between collected variables may go unappreciated because they were not...
Introduction: Blisibimod is a potent B cell-activating factor (BAFF) antagonist that binds to both cell membraneexpressed and soluble BAFF.The goal of these first-in-human studies was characterize the safety, tolerability, pharmacokinetic pharmacodynamic profiles blisibimod in subjects with systemic lupus erythematosus (SLE).Methods: SLE mild disease stable/inactive at baseline received either single dose (0.1, 0.3, 1, or 3 mg/kg subcutaneous [SC] 3, 6 intravenous [IV]) placebo (phase 1a; N...
SUMMARYObjective: To develop a validated BIACORE immunoassay for the detection and characterization of serum antibodies with specificity erythropoietic molecules (e.g. darbepoetin alfa).Methods: New Zealand White rabbits (n = 8) were immunized by an intramuscular injection alfa/adjuvant at 0,4,6, 8 weeks. Serum was collected 6 weeks after final pooled affinity purification. Antibody measurements performed using 3000 alfa immobilized to biosensor surface. Human samples spiked...
Background: Combined analysis of DNA content and immunofluorescence on single cells by flow cytometry provides information the proliferative response subpopulations to stimuli in mixed cell preparations; however, low-viability preparations, dead interfere with accurate cytometric data because nonspecific binding antibodies altered DNA-staining profiles. Light scatter differences between nonviable viable are unreliable, particularly after permeabilization step that is necessary for staining....
The host immune factors that determine susceptibility to HIV-1 infection are poorly understood. We compared multiple immunologic parameters in three groups of HIV-1-seronegative men: 14 highly exposed (HR10), 7 previously reported possibly have sustained transient (PTI), and a control group low risk blood bank donors (BB). Virus-specific cellular assays were performed for CD4(+) T helper cell responses, CD8(+) cytotoxic lymphocyte activity, chemokine release, cell-derived antiviral soluble...
We previously reported that human immunodeficiency virus type 1 (HIV-1), herpes simplex (HSV), and Sendai induce higher levels of alpha interferon (IFN-alpha) in blood dendritic cells than monocytes healthy donors. In the present study, IFN-alpha induced by T-cell tropic (IIIb RF) monocytotropic (BaL) strains HIV-1 HSV were significantly decreased peripheral mononuclear (PBMCs) derived from subjects with asymptomatic symptomatic infection. contrast, virus, a paramyxovirus induces...
RANTES (regulated on activation, normal T expressed and secreted), macrophage inflammatory protein (MIP)-1α, MIP-1β are human immunodeficiency virus (HIV) suppressor factors by virtue of their ability to compete with HIV for access cell surface R5. Their block infection in vitro is unequivocal; however, role as vivo not firmly established. We therefore conducted a study test the hypothesis that production these was correlate decreased burden vivo. Moreover, we asked whether higher β...
Non-alcoholic fatty liver disease (NAFLD), recently renamed metabolic dysfunction-associated steatotic (MASLD), is a progressive disorder that begins with aberrant triglyceride accumulation in the and can lead to cirrhosis cancer. A common variant gene