A5024238976- Endoplasmic Reticulum Stress and Disease
- Liver Disease Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Pancreatic function and diabetes
- Autophagy in Disease and Therapy
- Lipid metabolism and biosynthesis
- Immunotherapy and Immune Responses
- Diet and metabolism studies
- Protein Degradation and Inhibitors
- Drug Transport and Resistance Mechanisms
- Genetics, Bioinformatics, and Biomedical Research
- Image Processing Techniques and Applications
- Adipose Tissue and Metabolism
- vaccines and immunoinformatics approaches
- Cholesterol and Lipid Metabolism
- Circadian rhythm and melatonin
- Cellular transport and secretion
- Cell Image Analysis Techniques
- Dietary Effects on Health
- Peptidase Inhibition and Analysis
- Metabolism and Genetic Disorders
- Birth, Development, and Health
Amgen (United States)
2021-2024
California Institute of Technology
2016-2023
Baylor College of Medicine
2011-2016
Nonalcoholic fatty liver disease, recently renamed metabolic dysfunction-associated steatotic disease (MASLD), is a progressive disorder that begins with aberrant triglyceride accumulation in the and can lead to cirrhosis cancer. A common variant gene
Significance We have recently reported that glutamine synthetase (GS) is negatively regulated by through a feedback loop involving the E3 ubiquitin ligase CRL4 CRBN . However, molecular events take place at each step of pathway are not well understood. Here, we show valosin-containing protein (VCP)/p97, required for GS degradation. It acts downstream p97 extracts ubiquitylated subunits from decamer so they can be degraded proteasome. Interestingly, also immunomodulatory drug-induced...
Chronic endoplasmic reticulum (ER) stress results in toxicity that contributes to multiple human disorders. We report a resolution pathway initiated by the nuclear receptor LRH-1 is independent of known unfolded protein response (UPR) pathways. Like mice lacking primary UPR components, hepatic Lrh-1-null cannot resolve ER stress, despite functional UPR. In induces expression kinase Plk3, which phosphorylates and activates transcription factor ATF2. Plk3-null also restoring Plk3 cells rescues...
Protein quality control (PQC) plays an important role in stemming neurodegenerative diseases and is essential for the growth of some cancers. Valosin-containing protein (VCP)/p97 a pivotal multiple PQC pathways by interacting with numerous adaptors that link VCP to specific substrates influence post-translational modification state substrates. However, our poor understanding specificity architecture adaptors, dynamic properties their interactions hinders fundamental features how modulation...
Peptides from degradation of intracellular proteins are continuously displayed by major histocompatibility complex (MHC) class I. To better understand origins these peptides, we performed a comprehensive census the I peptide repertoire in presence and absence ubiquitin-proteasome system (UPS) activity upon developing optimized methodology to enrich for quantify peptides. Whereas most peptides dependent on UPS their generation, surprising 30%, enriched mitochondrial origin, appears...
Balance of labile methyl groups (choline, methionine, betaine, and folate) is important for normal liver function. Quantitatively, a significant use in the production phosphatidylcholines (PCs), which are ligands nuclear receptor homolog-1 (LRH-1). We studied role LRH-1 methyl-pool homeostasis determined its metabolic effects using methionine choline-deficient (MCD) diet, depletes results deleterious decrease PC-to-phosphatidylethanolamine ratio. found that MCD diet-fed, liver-specific...
Circadian rhythm regulates multiple metabolic processes and in turn is readily entrained by feeding-fasting cycles. However, the molecular mechanisms which peripheral clock senses nutrition availability remain largely unknown. Bile acids are under circadian control also increase postprandially, serving as regulators of fed state liver. Here, we show that nuclear receptor Small Heterodimer Partner (SHP), a regulator bile acid metabolism, impacts endogenous directly regulating Bmal1....
Non-alcoholic fatty liver disease (NAFLD), recently renamed metabolic dysfunction-associated steatotic (MASLD), is a progressive disorder that begins with aberrant triglyceride accumulation in the and can lead to cirrhosis cancer. A common variant gene
Despite the range of tasks performed by biological image-processing software, current versions cannot find matches for image in question among huge images that exist literature and elsewhere on Internet. Google's Reverse Image Search is designed this, it a simple, yet powerful tool can be applied to decipher contents images. For contain unfamiliar or unknown elements, example, identify similar features published Here we describe general guidelines using this freely available search National...
ABSTRACT The adaptive immune system distinguishes self from non-self by surveying peptides generated degradation of intracellular proteins that are loaded onto MHC Class I molecules for display on the cell surface. While early studies reported bulk surface complexes require ubiquitin-proteasome (UPS) their generation, this conclusion has been challenged. To better understand peptide origins, we sought to carry out a comprehensive, quantitative census repertoire in presence and absence UPS...