A5073306413- RNA Research and Splicing
- Genetic Mapping and Diversity in Plants and Animals
- Protist diversity and phylogeny
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- GABA and Rice Research
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- RNA regulation and disease
- Rice Cultivation and Yield Improvement
- Plant-Microbe Interactions and Immunity
- Fungal Biology and Applications
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolic Engineering and Bioproduction
- Neurogenetic and Muscular Disorders Research
- Bioinformatics and Genomic Networks
- RNA Interference and Gene Delivery
- Wheat and Barley Genetics and Pathology
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
Huaiyin Normal University
2021-2025
Second Affiliated Hospital of Fujian Medical University
2022-2024
Fujian Medical University
2022-2024
First Affiliated Hospital of Dalian Medical University
2021-2024
Dalian Medical University
2021-2024
Huazhong University of Science and Technology
2024
Central Hospital of Wuhan
2024
Nanjing Tech University
2023
Amgen (United States)
2023
Shenyang University of Technology
2023
Heterotrimeric G proteins are important transducers of receptor signaling, functioning in plants with CLAVATA receptors controlling shoot meristem size and pathogen-associated molecular pattern basal immunity. However, whether specific members the heterotrimeric complex potentiate cross-talk between development defense, extent to which these functions conserved across species, have not yet been addressed. Here we used CRISPR/Cas9 knock out maize protein β subunit gene (Gβ) found that mutants...
<h3>Objective:</h3> To identify the underlying genetic cause in a consanguineous Chinese family segregating distal hereditary motor neuropathy (dHMN) an autosomal recessive pattern. <h3>Methods:</h3> We used whole-exome sequencing and homozygosity mapping to detect variant 2 affected individuals of with dHMN. RNA analysis peripheral blood leukocytes immunofluorescence immunoblotting stable cell lines were performed support pathogenicity identified mutation. <h3>Results:</h3> 3 shared novel...
Based on the two-stage innovation value chain model, incorporating environmental regulation policies and three-waste emissions into research framework, constructing a green technology efficiency evaluation index system from perspectives of inputs outputs, utilizing super-efficiency SBM model Malmquist-Luenberger assessing effectiveness technological in two aspects, namely, static, dynamic, respectively.The results show that: average technical China's hightech industries is 0.5277, with...
Abstract Background Cofilin is a member of the actin depolymerizing factor (ADF)/cofilin family, which regulates dynamics. Increasing evidence suggests that mitochondrial translocation cofilin appears necessary for regulation apoptosis. Results We report allyl isothiocyanate (AITC) potently induces mitochondria injury and These events were accompanied by loss polymerized filamentous (F-actin) increase in unpolymerized globular (G-actin). AITC also dephosphorylation through activation PP1...
Most of the current alternative splicing (AS) analysis tools are powerless to analyse complex splicing. To address this, we developed SUVA (Splice sites Usage Variation Analysis) that decomposes events into five types splice junction pairs. By analysing real and simulated data, showed higher sensitivity accuracy in detecting AS than compared methods. Notably, detected extensive screened out 69 highly conserved dominant associated with cancer. The cancer-associated FN1 co-regulated...
Cell-free protein microarrays display naturally-folded proteins based on just-in-time
A total of 34 apple ( Malus × domestica Borkh.) cDNA libraries were constructed from root, leaf, bud, shoot, flower, and fruit tissues, at various developmental stages and/or under biotic or abiotic stress conditions, several genotypes. From these libraries, 190,425 clones partially sequenced the 5′ end 42,619 3′ end, a 182,241 high‐quality expressed sequence tags (ESTs) obtained. These coalesced into 23,442 tentative contigs 9843 singletons, for 33,825 unigenes. Functional annotation this...
// Yan Luo 1, 2, 3, * , Lei Liu Yang Wu 2 Karnika Singh Bing Su 4 Nan Zhang 1 Xiaowei Yangmei Shen Shile Huang 3 State Key Laboratory of Biotherapy/Collaborative Innovation Center Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic Department Biochemistry and Molecular Biology, Louisiana University Health Sciences Center, Shreveport, LA, USA Feist-Weiller Cancer Immunobiology, Yale School Medicine, New Haven, CT, These authors have contributed equally to...
Chloroplasts descended from cyanobacteria and have a drastically reduced genome following an endosymbiotic event. Many genes of the ancestral cyanobacterial been transferred to plant nuclear by horizontal gene transfer. However, selective set metabolism pathways is maintained in chloroplasts using both chloroplast encoded enzymes. As organelle specialized for carrying out photosynthesis, does metabolic network properties adapted higher efficiency photosynthesis? We compared prokaryotic...
Long noncoding RNAs (lncRNAs) play crucial roles in regulating numerous biological processes which complicated mechanisms are involved. Nonetheless, little is known about the number, features, sequences, and possible effects of lncRNAs on plant responses to alkaline stress.Leaf samples collected based control Beta vulgaris L., as well those under short-term long-term treatments, were subjected high-throughput RNA sequencing, through a total 8535 with reliable expression detected. Of these...
The heterotrimeric G-protein mediates growth and development by perceiving transmitting signals in multiple organisms. Alternative splicing (AS), a vital process for regulating gene expression at the post-transcriptional level, plays significant role plant adaptation evolution. Here, we identified five variants of Gγ subunit TaGS3 (TaGS3.1 to TaGS3.5), which showed divergence during wheat polyploidization, differential function grain weight size determination. TaGS3.1 overexpression...
Pre-mRNA splicing is a vital step in the posttranscriptional regulation of gene expression. Splicing catalyzed by spliceosome, multidalton RNA-protein complex, through two successive transesterifications to yield mature mRNAs. In Arabidopsis, more than 61% all transcripts from intron-containing genes are alternatively spliced, thereby resulting transcriptome and subsequent proteome diversities for cellular processes. Moreover, it estimated that alternative (AS) events induced adverse stimuli...
Grain size is subtly regulated by multiple signaling pathways in rice. Alternative splicing a general mechanism that regulates gene expression at the post-transcriptional level. However, to our knowledge, molecular underlying grain regulation alternative largely unknown. GS3, first identified QTL for rice, transcriptional and post-translational In this study, we GS3 subject splicing. GS3.1 GS3.2, two dominant isoforms, accounts about 50% 40% of total transcripts, respectively. encodes...
Non-alcoholic fatty liver disease (NAFLD), recently renamed metabolic dysfunction-associated steatotic (MASLD), is a progressive disorder that begins with aberrant triglyceride accumulation in the and can lead to cirrhosis cancer. A common variant gene
The research on the correlation or causality between gut microbiota and occurrence, development, treatment of colorectal cancer (CRC) is receiving increasing emphasis. At same time, incidence mortality vary among individuals regions, as does microbiota. In order to gain a better understanding characteristics in CRC patients differences different we initially compared 25 26 healthy controls central region China (Hubei Province) using 16S rRNA high-throughput sequencing technology. results...
Low temperature germination (LTG) is a key agronomic trait in rice (Oryza sativa L.). However, the genetic basis of natural variation for LTG largely unknown. Here, genome-wide association study (GWAS) was performed using 276 accessions from 3,000 Rice Genomes (3K-RG) project with 497 k single nucleotide polymorphisms (SNPs) to uncover potential genes rice. In total, 37 quantitative loci (QTLs) 6th day (D6) 10th (D10) were detected full population, overlapping 12 previously reported QTLs...
Neural salient serine-/arginine-rich protein 1 (NSSR1) is a newly identified SR that regulates pre-mRNA splicing. In the present study, we demonstrated neural specialization of NSSR1 expression in humans and mice. Strong immunoreactive signals to were observed mouse cerebral neurons, cerebellar Purkinje cells, pyramidal neurons CA1, CA2 CA3 regions hippocampus granule cells dentate gyrus. primarily cultured progenitor (NPCs), at undifferentiated status, transcripts detected, but not...