A5101735405- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
- SARS-CoV-2 and COVID-19 Research
- Invertebrate Immune Response Mechanisms
- Microplastics and Plastic Pollution
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Long-Term Effects of COVID-19
- Animal Genetics and Reproduction
- Cancer Research and Treatments
- Trauma Management and Diagnosis
- Assisted Reproductive Technology and Twin Pregnancy
- Physiological and biochemical adaptations
- Reproductive biology and impacts on aquatic species
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Genetic Syndromes and Imprinting
- Nanoparticles: synthesis and applications
- Antioxidant Activity and Oxidative Stress
- Flavonoids in Medical Research
- Gynecological conditions and treatments
Tianjin University of Science and Technology
2020-2024
Chery Automobile (China)
2023
Beijing Proteome Research Center
2017-2020
Chinese Academy of Medical Sciences & Peking Union Medical College
2020
Institute of Oceanology
2011-2018
Chinese Academy of Sciences
2011-2018
Qingdao National Laboratory for Marine Science and Technology
2018
Dana-Farber Cancer Institute
2017
University of Chinese Academy of Sciences
2011-2014
Qingdao University of Science and Technology
2014
Comprehensive profiling of humoral antibody response to severe acute respiratory syndrome (SARS) coronavirus-2 (CoV-2) proteins is essential in understanding the host immunity and developing diagnostic tests vaccines. To address this concern, we developed a SARS-CoV-2 proteome peptide microarray analyze interactions at amino acid resolution. With array, demonstrate feasibility employing SARS-CoV-1 antibodies detect nucleocapsid phosphoprotein. The first landscape B-cell epitopes for IgM IgG...
Serum and plasma contain abundant biological information that reflect the body's physiological pathological conditions are therefore a valuable sample type for disease biomarkers. However, comprehensive profiling of serological proteome is challenging due to wide range protein concentrations in serum. Methods: To address this challenge, we developed novel in-depth serum proteomics platform capable analyzing across ~10 orders or magnitude by combining data obtained from Data Independent...
Abstract COVID-19 has quickly become a worldwide pandemic, which significantly impacted the economy, education, and social interactions. Understanding humoral antibody response to SARS-CoV-2 proteins may help identify biomarkers that can be used detect treat infection. However, no immuno-proteomics platform exists perform such proteome-wide analysis. To address this need, we created proteome microarray analyze interactions at amino acid resolution by spotting peptides 15 acids long with...
Background: Programmed cell death protein 1 (PD1) inhibitors have revolutionized cancer therapy, yet many patients fail to respond.Thus, the identification of accurate predictive biomarkers therapy response will improve clinical benefit anti-PD1 therapy.Method: We assessed baseline serological autoantibody (AAb) profile against ~2300 proteins in 10 samples and ~4600 35 with alveolar soft part sarcoma (ASPS), non-small-cell lung (NSCLC) lymphoma using Nucleic Acid Programmable Protein Arrays...
Cell-free protein microarrays display naturally-folded proteins based on just-in-time
Abstract Rapid and accurate tests that detect IgM IgG antibodies to SARS-CoV-2 proteins are essential in slowing the spread of COVID-19 by identifying patients who infected with COVID-19. Using a proteome microarray developed our lab, we comprehensively profiled both forty early-stage COVID-19, influenza, or non-influenza had similar symptoms. The results revealed N protein is not an ideal biomarker for diagnosis because its low immunogenicity, thus rely on this marker alone will have high...
Zhikong scallop (Chlamys farreri) is one of the most economically important aquaculture species in China. Physical maps are crucial tools for genome sequencing, gene mapping and cloning, genetic improvement selective breeding. In this study, we have developed a genome-wide, BAC-based physical map species. A total 81,408 clones from two BAC libraries were fingerprinted using an ABI 3130xl Genetic Analyzer fingerprinting kit our laboratory. After data processing, 63,641 (∼5.8× coverage)...
Cell-free protein microarrays represent a special form of microarray which display proteins made fresh at the time experiment, avoiding storage and denaturation. They have been used increasingly in basic translational research over past decade to study protein-protein interactions, pathogen-host relationship, post-translational modifications, antibody biomarkers different human diseases. Their role first blood-based diagnostic test for early stage breast cancer highlights their value...
Embryonic diapause is a common evolutionary adaptation observed across wide range of organisms. Artemia one the classic animal models for research. The current studies mainly focus on induction and maintenance embryonic diapause, with little research molecular regulatory mechanism reactivation. first 5 h after breaking has been proved to be most important reactivation in Artemia. In this work, two high-throughput sequencing methods, ATAC-seq RNA-seq, were integrated study signal regulation...
The halophilic zooplankton brine shrimp Artemia has been used as an experimental animal in multidisciplinary studies. However, the reproductive patterns and its regulatory mechanisms remain unclear. In this study, ovarian development process of parthenogenetic (A. parthenogenetica) was divided into five stages, oogenesis or egg formation identified six phases. mode assumed to be polytrophic. We also traced dynamic translocation candidate germline stem cells (cGSCs) using EdU labelling...
Half-smooth tongue sole Cynoglossus semilaevis is a high-value marine fish species. The intensive farming of this flatfish species has been suffered from disease outbreak. Poly-β-hydroxybutyrate (PHB) carbon storage compound accumulated by wide variety prokaryotes and considered biocontrol agent applied in aquaculture. In study, the dietary effect PHB-accumulating Halomonas (HM.PHB) was investigated on survival, growth, resistance Vibrio anguillarum intestine microbial composition C....
Archaeal carotenoids, as novel bioactive compound, are stronger free radical scavenger. The effects of archaea Halorubrum carotenoids on skin coloration, antioxidative status and immune-related genes expression golden trout Oncorhynchus mykiss were investigated. In total, 420 fish (10.90 ± 0.06 g) divided into three groups with four replicates, fed commercial diets containing 0 (Ctrl), 25 mg/kg (P25) 50 mg archaeal carotenoids/kg feed (P50) for 8 weeks. Although no significant difference was...