A5100647689- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Cancer Mechanisms and Therapy
- Advanced biosensing and bioanalysis techniques
- Computational Drug Discovery Methods
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- ATP Synthase and ATPases Research
- SARS-CoV-2 detection and testing
- RNA and protein synthesis mechanisms
- Endometrial and Cervical Cancer Treatments
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Renal and related cancers
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- interferon and immune responses
- Cancer Genomics and Diagnostics
- PI3K/AKT/mTOR signaling in cancer
- Lung Cancer Treatments and Mutations
- Uterine Myomas and Treatments
- Plant-based Medicinal Research
Northeastern University
2017-2025
Foshan University
2024-2025
Beijing University of Chinese Medicine
2025
Dalian University of Technology
2025
Universidad del Noreste
2020-2024
Stomatology Hospital
2024
Zhejiang University
2024
China-Japan Friendship Hospital
2024
Sichuan University
2021-2023
Jinzhou Medical University
2023
Significance Alternative RNA splicing and the spliceosome machinery have been implicated in cancer progression. A genome-wide CRISPR screen identified processing factor heterogeneous nuclear ribonucleoprotein L (HNRNPL) as required for prostate growth by regulating alternative circular formation. HNRNPL its clients are overexpressed during progression, supporting their potential role therapeutic targets.
CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise at the single-base resolution its wide applications including high-fidelity SNP genotyping remain be explored. Here we develop a Cas12b-mediated (CDetection) strategy, which shows higher sensitivity on examined targets compared with previously Cas12a-based platform. Moreover, show that CDetection can distinguish differences level upon combining optimized tuned guide RNA...
The prokaryotic CRISPR-Cas adaptive immune systems provide valuable resources to develop genome editing tools, such as CRISPR-Cas9 and CRISPR-Cas12a/Cpf1. Recently, CRISPR-Cas12b/C2c1, a distinct type V-B system, has been characterized dual-RNA-guided DNA endonuclease system. Though being active in vitro, its cleavage activity at endogenous remains be explored. Furthermore, the optimal temperature of reported Cas12b orthologs is higher than 40 °C, which unsuitable for mammalian applications....
Comprehensive profiling of humoral antibody response to severe acute respiratory syndrome (SARS) coronavirus-2 (CoV-2) proteins is essential in understanding the host immunity and developing diagnostic tests vaccines. To address this concern, we developed a SARS-CoV-2 proteome peptide microarray analyze interactions at amino acid resolution. With array, demonstrate feasibility employing SARS-CoV-1 antibodies detect nucleocapsid phosphoprotein. The first landscape B-cell epitopes for IgM IgG...
CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides promising tool for genome engineering. However, only three Cas12a orthologs have been employed mammalian editing, and the editing efficiency as well targeting coverage still requires improvements. Here, we harness six novel in human mouse cells, some of which utilize simple protospacer adjacent motifs (PAMs) that remarkably increase range genomes. Moreover, identify optimized CRISPR RNA (crRNA) scaffolds can Cas12a.
Abstract A major challenge in the application of CRISPR-Cas13d system is to accurately predict its guide-dependent on-target and off-target effect. Here, we perform proliferation screens design a deep learning model, named DeepCas13, activity from guide sequences secondary structures. DeepCas13 outperforms existing methods efficiency guides targeting both protein-coding non-coding RNAs. Guides non-essential genes display viability effects, which are closely related their efficiencies....
Resistance to chemotherapy has been a major hurdle that limits therapeutic benefits for many types of cancer. Here we systematically identify genetic drivers underlying chemoresistance by performing 30 genome-scale CRISPR knockout screens seven chemotherapeutic agents in multiple cancer cells. Chemoresistance genes vary between conditions primarily due distinct background and mechanism action drugs, manifesting heterogeneous multiplexed routes towards chemoresistance. By focusing on...
Embryonic stem (ES) cells are under precise control of both intrinsic self-renewal gene regulatory network and extrinsic growth factor-triggered signaling cascades. How external pathways connect to core transcriptional circuits is largely unknown. To probe this, we chose BMP signaling, which previously recognized as a master for lineage commitment murine ES cells. Here, mapped target promoter occupancy SMAD1/5 SMAD4 on genome-wide scale found that they associate with large group...
Dual‑specificity phosphatase‑1 (DUSP1) is an oncogene that associated with cancer progression following drug resistance. In order to investigate the potential relationship between DUSP1 and apatinib resistance in gastric cells, we preformed many assays study this problem. gene was detected by RT‑qPCR assay, proteins MAPK pathway were quantified western blot CCK‑8 flow cytometry Hoechest 33342 stain performed detect of cell cycles apoptosis, respectively. Immunohistochemical staining used...
Abstract COVID-19 has quickly become a worldwide pandemic, which significantly impacted the economy, education, and social interactions. Understanding humoral antibody response to SARS-CoV-2 proteins may help identify biomarkers that can be used detect treat infection. However, no immuno-proteomics platform exists perform such proteome-wide analysis. To address this need, we created proteome microarray analyze interactions at amino acid resolution by spotting peptides 15 acids long with...
Significance We identified a group of DNA repair genes directly induced by EZH2 and repressed inhibitors. Expression these predicts the response wild-type EZH2-harboring solid tumors to Most importantly, our findings lay foundation for development combination therapy that combines inhibitors damaging agents or drugs block treatment castration-resistant prostate cancer other tumors.
Abstract Objective To determine the ability of contrast‐enhanced magnetic resonance imaging to predict myometrial invasion, cervical and pelvic lymph node metastasis in endometrial carcinoma analyze factors that lead errors this identification. Design A retrospective study. Setting University general hospital. Population total 167 women diagnosed with carcinoma. Methods All patients received a preoperative scan. Histopathological findings were used as definitive diagnosis. Main outcome...
Trametes robiniophila Murr. (Huaier) is a widely used anti-cancer agent in China. Strong evidence for the anti-proliferative activity of Huaier has been reported; however, its anti-metastatic potential against gastric cancer (GC) as well underlying mechanism action are unknown. Here, we show that treatment with an aqueous extract over range concentrations significantly suppressed both invasiveness and migratory ability GC cells. could also partly reverse epithelial-mesenchymal transition...
Early-onset lung cancer in young adults represents a less studied clinical entity with increasing incidence which still affects large number of patients. Here we perform comprehensive analysis early-onset for the clinicopathological features, genomic alterations, gene expression, and immune landscape by establishing cohort enrolling 421 non-small cell (NSCLC) patients from ten medical centers China. Comparative reveals distinct alteration between younger elder NSCLC, ERBB2 mutations...
Emerging research suggested a potential role of immune cells in colorectal cancer (CRC) development. However, the causal relationship between phenotypes and CRC remains elusive. Hence, this two-sample Mendelian randomization (MR) study aimed to explore association. In study, bidirectional, MR analysis multivariate was conducted, leveraging public genetic data. Four types were employed. A comprehensive sensitivity carried out validate robustness, heterogeneity, horizontal pleiotropy results,...
Abstract Background Breast cancer is one of the most common cancers in women, with a notably high mortality rate. Early diagnosis can improve survival rates. However, early‐stage breast tumors suffer challenges for accurate detection and are hard to detect due their tiny sizes blurry edges, thereby obtaining degraded performance. Purpose To solve above issues, this study aims develop robust model tumor segmentation from magnetic resonance imaging (MRI) provide quality assessment cancer....