A5101531327- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- DNA and Nucleic Acid Chemistry
- Traditional Chinese Medicine Analysis
- Computational Drug Discovery Methods
- Pharmacological Effects of Natural Compounds
- RNA regulation and disease
- RNA Research and Splicing
- Tea Polyphenols and Effects
- Metabolism, Diabetes, and Cancer
- Natural product bioactivities and synthesis
- Fibroblast Growth Factor Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Silk-based biomaterials and applications
- Higher Education and Teaching Methods
- RNA and protein synthesis mechanisms
- Enhanced Oil Recovery Techniques
- Phytochemicals and Antioxidant Activities
- Epigenetics and DNA Methylation
- Reservoir Engineering and Simulation Methods
- Plant-based Medicinal Research
- Pancreatic function and diabetes
Zhengzhou Central Hospital
2022-2024
First Affiliated Hospital of Zhengzhou University
2022
Shanghai Clinical Research Center
2022
Shanghai University
2022
Cold Spring Harbor Laboratory
2019
Institute of Subtropical Agriculture
2018
Hangzhou Vocational and Technical College
2018
Xiangnan University
2018
Second Military Medical University
2016-2017
Eastern Hepatobiliary Surgery Hospital
2017
In the Drosophila germline, repeat-associated small interfering RNAs (rasiRNAs) ensure genomic stability by silencing endogenous selfish genetic elements such as retrotransposons and repetitive sequences. Whereas (siRNAs) derive from both sense antisense strands of their double-stranded RNA precursors, rasiRNAs arise mainly strand. rasiRNA production appears not to require Dicer-1, which makes microRNAs (miRNAs), or Dicer-2, siRNAs, lack 2′,3′ hydroxy termini characteristic animal siRNA...
Small interfering RNAs (siRNAs) direct RNA interference (RNAi) in eukaryotes. In flies, somatic cells produce siRNAs from exogenous double-stranded (dsRNA) as a defense against viral infection. We identified endogenous (endo-siRNAs), 21 nucleotides length, that correspond to transposons and heterochromatic sequences the of Drosophila melanogaster . also detected endo-siRNAs complementary messenger (mRNAs); these disproportionately mapped regions overlapping mRNAs predicted form vivo. Normal...
PIWI-interacting RNAs (piRNAs) protect the animal germ line by silencing transposons. Primary piRNAs, generated from transcripts of genomic transposon "junkyards" (piRNA clusters), are amplified "ping-pong" pathway, yielding secondary piRNAs. We report that bound to PIWI protein Ago3, can initiate primary piRNA production cleaved RNAs. The first ~26 nucleotides (nt) each RNA becomes a piRNA, but subsequent nt become in series phased piRNAs bind Piwi, allowing spread beyond site cleavage....
Recombinant adeno-associated viruses (rAAVs) that can cross the blood-brain-barrier and achieve efficient stable transvascular gene transfer to central nervous system (CNS) hold significant promise for treating CNS disorders. However, following intravascular delivery, these vectors also target liver, heart, skeletal muscle, other tissues, which may cause untoward effects. To circumvent this, we used tissue-specific, endogenous microRNAs (miRNAs) repress rAAV expression outside CNS, by...
Abstract Background Oxidative stress (OS) and inflammatory reactions play pivotal roles in secondary brain injury after traumatic (TBI). Histone deacetylase 3 (HDAC3) controls the acetylation of histones non-histones, which has a significant impact on central nervous system’s reaction to damage. This research determined implications RGFP966, new specific inhibitor HDAC3, for antioxidant (AO) systems mediated by nuclear factor erythroid2-related 2 (Nrf2) Nod-like receptor protein (NLRP3)...
BACKGROUND:This study aimed to investigate the regulatory effects of L-theanine on secretion immune cytokines, hormones, and neurotransmitters, mRNA expression phospholipase C (PLC) in rats, explore its mechanism function. MATERIAL AND METHODS:Sixty-four Sprague-Dawley rats received daily intragastric infusion different doses solution [0, 50 (LT), 200 (MT), 400 (HT) mg/kg BW]. Cytokines, immunoglobulins, hormones serum, PLC relevant tissues were assayed. RESULTS:L-theanine administration...
Abstract Combination therapy is a popular treatment for various diseases in the clinic. Among successful cases, Traditional Chinese Medicinal (TCM) formulae can achieve synergistic effects therapeutics and antagonistic toxicity. However, characterizing underlying molecular synergisms combination of drugs remains challenging task due to high experimental expenses complication multicomponent herbal medicines. To understand rationale therapy, we investigated Sini Decoction, well-known TCM...
BACKGROUND The cortex of Magnolia officinalis has long been used as an element traditional Chinese medicine for the treatment anxiety, chronic bronchitis, and gastrointestinal dysfunction. This study aimed to elucidate underlying mechanism its functional ingredients (magnolol honokiol) in modifying secretion absorption homeostasis protecting mucosal integrity Enterotoxigenic Escherichia coli (ETEC)-induced diarrhea mouse model. MATERIAL AND METHODS established a model infected by ETEC at...
BACKGROUND L-theanine is a non-protein amino acid in green tea, and its hepatoprotection neuroprotection have been verified. However, whether can prevent cardiomyocytes from apoptosis unclear yet. This study evaluated the protective effects of on H2O2-induced heart injury vitro. MATERIAL AND METHODS The certified H9C2 cells were pretreated with (0 mM, 4 8 16 mM) for 24 h, followed by 160 µM H2O2 solution h. cell viability antioxidant indices assayed. Quantitative evaluation was performed...
Short hairpin RNAs that are delivered by recombinant adeno-associated virus (rAAV) have the potential to elicit long-term RNAi therapy for human disease. However, discovery short sequences can cause truncation of rAAV genome calls into question efficiency and gene-silencing specificity this strategy in humans. Here, we report embedding guide strand a small silencing RNA an artificial microRNA (miRNA) scaffold derived from mouse miRNA-33 ensures genomic integrity reduces off-targeting...
The purpose of this study was to explore the risk factors for renal nonrecovery among elderly and nonelderly patients with acute kidney injury (AKI) in critically ill patients. A multicenter retrospective cohort 583 AKI examined. We found best cutoff value predicting recovery by age 63 years old through logistic regression. All were divided into two cohorts, <63 ≥63-years old; on basis at 30 days after AKI, patient cohorts further a group group. Multivariate regression used analyze affecting...
Small silencing RNAs have provided powerful reverse genetics tools and opened new areas of research. This introduction describes the use RNAi to suppress expression individual genes for loss-of-function analysis. It also summarizes methods measuring specific global changes in small RNA expression, as well inhibit function endogenous species such miRNAs.
This protocol is used to design antisense oligonucleotides (ASOs) for specific inhibition of miRNA function in cultured cells. The incorporation 2′- O -methyl-modifications ASOs enhances their potency and resistance degradation, whereas 3′-terminal cholesterol-conjugation facilitates delivery into
This protocol describes how to use northern hybridization detect 15- 150-nt small RNAs. Total RNA is fractionated by electrophoresis through a denaturing polyacrylamide gel and then transferred nylon membrane semidry electroblotting. After UV-cross-linking the membrane, performed in Church buffer using 32P-radiolabeled oligonucleotide probe followed PhosphorImager analysis. The of StarFire probes allows substantial increase specific radioactivity probe. contain two domains. target-specific...
This is a simple and widely used method for the preparation of dsRNA using polymerase chain reaction (PCR) template-based, in vitro transcription reaction. The resulting then to trigger RNAi an appropriate cell or organism.
Background and Objective. The morbidity mortality rates of non-small cell lung cancer (NSCLC) remain high. Zhenqi Fuzheng (ZQFZ) granule, which consists Astragali Radix Ligustri Lucidi Fructus, is commonly used to improve the immunity patients. However, mechanism ZQFZ granule against NSCLC still unclear. In this study, network pharmacology molecular docking approaches were investigate potential on NSCLC. Methods. ingredients in considered one study based UPLC, targets predicted...
Long dsRNA cannot be used in most cultured mammalian cells because it triggers the interferon response, causing widespread changes gene expression and apoptosis. This protocol describes a method for delivering into siRNA duplexes that are too short to elicit sequence-nonspecific responses associated with long dsRNA.