- Marine Biology and Environmental Chemistry
- Marine Bivalve and Aquaculture Studies
- Marine Ecology and Invasive Species
- Invertebrate Immune Response Mechanisms
- Aquaculture disease management and microbiota
- Developmental Biology and Gene Regulation
- Aquaculture Nutrition and Growth
- Calcium Carbonate Crystallization and Inhibition
- Protein Hydrolysis and Bioactive Peptides
- Marine Invertebrate Physiology and Ecology
- Molecular Biology Techniques and Applications
- Genetic diversity and population structure
- Cephalopods and Marine Biology
- Animal Genetics and Reproduction
- Bone Tissue Engineering Materials
- Genomics and Phylogenetic Studies
- Heat shock proteins research
- CRISPR and Genetic Engineering
- Trace Elements in Health
- Cellular Mechanics and Interactions
- RNA Research and Splicing
- Marine Biology and Ecology Research
- TGF-β signaling in diseases
- Aquatic Invertebrate Ecology and Behavior
- Advanced biosensing and bioanalysis techniques
Institute of Oceanology
2015-2024
Chinese Academy of Sciences
2015-2024
Qingdao National Laboratory for Marine Science and Technology
2017-2024
University of Chinese Academy of Sciences
2010-2024
Academia Sinica
2020
Institute of Oceanology
2011
Abstract Reconstructing the genomes of bilaterian ancestors is central to our understanding animal evolution, where knowledge from ancient and/or slow-evolving lineages critical. Here we report a high-quality, chromosome-anchored reference genome for scallop Patinopecten yessoensis , bivalve mollusc that has with many ancestral features. Chromosome-based macrosynteny analysis reveals striking correspondence between 19 chromosomes and 17 presumed linkage groups at level conservation...
Apart from sharing common ancestry with chordates, sea cucumbers exhibit a unique morphology and exceptional regenerative capacity. Here we present the complete genome sequence of an economically important cucumber, A. japonicus, generated using Illumina PacBio platforms, to achieve assembly approximately 805 Mb (contig N50 190 Kb scaffold 486 Kb), 30,350 protein-coding genes high continuity. We used this resource explore key genetic mechanisms behind biological characters cucumbers....
In contrast to the Hox genes in arthropods and vertebrates, those molluscs show diverse expression patterns with differences reported among lineages. Here, we investigate 2 phylogenetically distant molluscs, a gastropod polyplacophoran, that both species can be divided into categories. The ventral ectoderm generally shows canonical staggered pattern comparable of other bilaterians likely contributes patterning, such as neurogenesis. category on dorsal side is strongly correlated shell...
The early development of mollusks exhibits important characteristics from the developmental and evolutionary perspective. With increasing number genome-wide studies, accurate analyses quantitative gene expression during are impeded by lack validated reference genes. To improve situation, in this study, we analyzed stability seven candidate housekeeping genes Pacific oyster Crassostrea gigas: actin, glyceraldehyde-3-phosphate dehydrogenase (gapdh), α subunit elongation factor 1 (elf1α),...
Abstract Although a conserved mechanism relying on BMP2/4 and Chordin is suggested for animal dorsal–ventral (DV) patterning, this has not been reported in spiralians, one of the three major clades bilaterians. Studies limited spiralian representatives have markedly diverse DV patterning mechanisms, considerable number which no longer deploy BMP signaling. Here, we showed that regulate mollusk Lottia goshimai, was predicted spiralians but previously reported. In context reports it conversely...
Zhikong scallop (Chlamys farreri) is one of the most economically important aquaculture species in China. Physical maps are crucial tools for genome sequencing, gene mapping and cloning, genetic improvement selective breeding. In this study, we have developed a genome-wide, BAC-based physical map species. A total 81,408 clones from two BAC libraries were fingerprinted using an ABI 3130xl Genetic Analyzer fingerprinting kit our laboratory. After data processing, 63,641 (∼5.8× coverage)...
A total of 2,970 EST–SSRs (2.38%) were identified by transcriptome sequencing clam Meretrix meretrix (751,970 reads, ~310.82 Mbp), using 454 Genome Sequencer FLX next-generation platform. Dinucleotide SSR was the dominant repeat type (40.2%), followed trinucleotide (37.8%), tetranuleotide (12.0%) and pentanucleotide (2.0%) SSR. The motif AT (71.3%) in dinucleotide AAC (45.6%) type. Nearly 79% all microsatellites had flanking sequences suitable for PCR primer design. Half PAL found to be...
Current understandings on the molecular mechanisms underlying bivalve metamorphosis are still fragmentary, and a comprehensive description is required. In this study, using large-scale label-free proteomic approach, we described compared proteomes of competent larvae (CL) juveniles (JU) Pacific oyster, Crassostrea gigas. A total 788 proteins were identified: 392 in CL proteome 636 JU proteome. Gene Ontology analysis from each sample revealed active metabolic processes both stages. Further...
A worldwide increase in the reports of diseases affecting marine organisms has paralleled climate warming over past few decades. In this study, we applied omics to explore mechanisms underlying thermo-linked epizootics, by comparing both transcriptome- and proteome-wide response turbots a mimic pathogen (poly I:C) between high temperature low using time-course approach. Our results showed that myeloperoxidase (MPO) insulin were differentially expressed transcripts shared all five time-points...