A5027997274- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Renal Transplantation Outcomes and Treatments
- Cytomegalovirus and herpesvirus research
- Organ Transplantation Techniques and Outcomes
- T-cell and Retrovirus Studies
- Sphingolipid Metabolism and Signaling
- HIV/AIDS Research and Interventions
- CAR-T cell therapy research
- Organ Donation and Transplantation
- Dialysis and Renal Disease Management
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Galectins and Cancer Biology
- RNA Interference and Gene Delivery
- Renal Diseases and Glomerulopathies
- Glycosylation and Glycoproteins Research
- Neurological Complications and Syndromes
- Blood groups and transfusion
- Muscle and Compartmental Disorders
University of California, Los Angeles
2011-2023
Black AIDS Institute
2004-2023
UCLA Jonsson Comprehensive Cancer Center
2000-2018
Amsterdam UMC Location University of Amsterdam
2004-2016
University of Amsterdam
2004-2016
APLA Health
2013-2015
Laboratory of Molecular Genetics
2002-2015
Olive View-UCLA Medical Center
2015
Leiden University Medical Center
2015
UCLA Health
2014
Abstract Identification of nonviable cells in immunofluorescently stained cell populations is essential for obtaining accurate data. Fluorescent non‐vital DNA dyes, particularly propidium iodide (PI), have been used routinely flow cytometry discrimination dead from viable on the basis fluorescence. We describe here use an alternative dye, 7‐amino‐actinomycin D (7‐AAD), which can replace PI exclusion cells. As example, we present this paper utilization 7‐AAD various leukemic lines whenever...
We found previously that Id3, which inhibits transcriptional activities of many basic helix-loop-helix transcription factors, blocked T and B cell development but stimulated natural killer (NK) development. Here we report ectopic expression Id3 another Id protein, Id2, strongly inhibited the primitive CD34+CD38− progenitor cells into CD123high dendritic (DC)2 precursors. In contrast, CD4+CD14+ DC1 precursors mature was not affected by Id2 or expression. These observations support notion a...
Galectin-1, a β-galactoside binding protein, is produced by thymic epithelial cells and binds to human thymocytes. We have previously reported that galectin-1 induces the apoptosis of activated T lymphocytes. Because majority thymocytes die via while still within thymus, we tested whether could induce these cells. now report in vitro exposure induced two subsets CD4lo CD8lo The phenotypes susceptible were consistent with both negatively selected nonselected Galectin-1–induced was enhanced...
Thymic epithelial cells play a crucial role in the selection of developing thymocytes. Thymocyte-epithelial cell interactions involve number adhesion molecules, including members integrin and immunoglobulin superfamilies. We found that human thymic synthesize an endogenous lectin, galectin-1, which binds to oligosaccharide ligands on surface thymocytes T lymphoblastoid cells. Binding was inhibited by antibody galectin-1 cells, two antibodies, T305 2B11, recognize carbohydrate epitopes...
Abstract A method was developed for gentle fixation of mammalian cells and permeabilization their membranes. The is useful staining intracellular antigens or quantification DNA content simultaneously with cell surface staining. Cells are treated 1 h at 4°C 0.25% buffered paraformaldehyde then 15 min 37°C 0.2% Tween 20 detergent in PBS. procedure permits excellent proteins, very low coefficients variation (CV) on the G 0 ‐peak distributions, preservation integrity antigens. vs. 90° angle...
Abstract A rapid, gentle, and sensitive method for quantification of cells undergoing apoptosis is presented. The allows the simultaneous determination dual‐color cell surface immunofluorescence. Cells are stained 7 min with vital dye Hoechst 33342 (HO342) identification live apoptotic cells. 7‐amino‐actinomycin D (7‐AAD) added to distinguish that have lost membrane integrity from Due its spectral properties 7‐AAD can be utilized on dual‐surface labelled fluoresceinisothiocyanate (FITC)...
Understanding how human activities influence immune response to environmental stressors can support biodiversity conservation across increasingly urbanizing landscapes. We studied a bobcat ( Lynx rufus ) population in urban southern California that experienced rapid decline from 2002–2005 due notoedric mange. Because anticoagulant rodenticide (AR) exposure was an underlying complication mange deaths, we aimed understand sublethal contributions of urbanization and ARs on 65 biochemical...
Gammaherpesviruses establish life-long persistency inside the host and cause various diseases during their persistent infection. However, systemic interaction between virus in vivo has not been studied individual hosts continuously, although such information can be crucial to control infection of gammaherpesviruses. For noninvasive continuous monitoring gammaherpesvirus host, a recombinant murine 68 (MHV-68, 68) was constructed express firefly luciferase gene driven by viral M3 promoter...
T cell surface sialylation changes during maturation in the thymus. We have previously demonstrated increased expression of mRNA encoding Galβ1, 3GalNAc α2,3-sialyltransferase mature medullary human thymocytes, compared with immature cortical thymocytes. For this enzyme, transferase correlated O-glycans. now examined pattern thymus two additional sialyltransferases, Galβ1,4GlcNAc α2,6-sialyltransferase (ST6N) and Galβ1,3/4GlcNAc (ST3N). The patterns were binding sialic acid-specific plant...
Abstract Plasmacytoid dendritic cells (pDC) are the principal producers of IFN-α in response to viral infection. Because pDC present thymus, we investigated consequences HIV-1-induced production by thymic pDC. We observed that as well thymocytes express intracellular upon infection with HIV-1. However, only could suppress HIV-1 replication, because depletion resulted enhancement replication thymocytes. Thymic also produce CpG oligonucleotides, consistent observations others peripheral...
ABSTRACT The goal of this study was to develop a small-animal model human immunodeficiency virus type 1 (HIV-1) pathogenesis in blood and primary secondary lymphoid organs. Rag2 −/− γ c mice that are neonatally injected with CD34 + cells functional immune system (HIS), hematopoietic being found the thymuses, peripheral blood, spleens, bone marrow animals (hereafter these referred as HIS-Rag2 mice). were infected small amounts CCR5-tropic HIV-1. Viral replication immunophenotypic changes...
During the past decade, as hemodialysis and renal transplantation have become more available, outlook for children with irreversible insufficiency has changed dramatically. Prior to that time, no programs were devoted exclusively treatment of end-stage disease (ESRD), a limited number received in adult facilities. In light very experience there those who claimed1,2 rigors program so catastrophic both child family, dismal, dialysis should not be offered children. Despite these warnings was...