A5047367075- Retinal Development and Disorders
- Pancreatic and Hepatic Oncology Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Gallbladder and Bile Duct Disorders
- CRISPR and Genetic Engineering
- Biliary and Gastrointestinal Fistulas
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Developmental Biology and Gene Regulation
- Single-cell and spatial transcriptomics
- Gastrointestinal disorders and treatments
- Congenital Anomalies and Fetal Surgery
- Zebrafish Biomedical Research Applications
- Amoebic Infections and Treatments
- Cardiac pacing and defibrillation studies
- Cardiac electrophysiology and arrhythmias
- Esophageal and GI Pathology
- Axon Guidance and Neuronal Signaling
- Pancreatitis Pathology and Treatment
- Gastric Cancer Management and Outcomes
- Pluripotent Stem Cells Research
- Parasitic infections in humans and animals
- Cardiovascular Function and Risk Factors
- Pelvic and Acetabular Injuries
University of Geneva
2023-2024
Montreal Clinical Research Institute
2017-2023
Université de Montréal
2017-2023
Johns Hopkins Medicine
2017-2021
Johns Hopkins University
2017-2021
Institute of Post Graduate Medical Education and Research
2018-2020
University of Pittsburgh Medical Center
2018-2019
Regional Medical Center
2019
Baystate Medical Center
2019
California University of Pennsylvania
2018
Retinal ganglion cells (RGCs) relay visual information from the eye to brain. RGCs are first cell type generated during retinal neurogenesis. Loss of function transcription factor Atoh7, expressed in multipotent early neurogenic progenitors leads a selective and essentially complete loss RGCs. Therefore, Atoh7 is considered essential for conferring competence on generate Despite importance RGC specification, we find that inhibiting apoptosis Atoh7-deficient mice by Bax only modestly reduces...
Multipotent retinal progenitor cells (RPCs) generate various cell types in a precise chronological order, but how exactly cone photoreceptor production is restricted to early stages remains unclear. Here, we show that the POU-homeodomain factors Pou2f1/Pou2f2, homologs of Drosophila temporal identity nub/pdm2, regulate timely cones mice. Forcing sustained expression Pou2f1 or Pou2f2 RPCs expands period production, whereas misexpression late-stage triggers ectopic at expense late-born fates....
Temporal identity factors are sufficient to reprogram developmental competence of neural progenitors and shift cell fate output, but whether they can also the terminally differentiated cells is unknown. To address this question, we designed a conditional gene expression system that allows rapid screening potential reprogramming in mouse retinal glial combined with genetic lineage tracing. Using assay, found coexpression early temporal transcription Ikzf1 Ikzf4 directly convert Müller (MG)...
The cerebral cortex shows species-specific variations in size and organization, likely accounting for distinct behavioral abilities. These structural differences may reflect evolutionary changes the developmental expression of shared genes. To investigate this possibility, we compared cell-type-specific gene across species developing mouse human neocortex, cortical organoids, by a transcriptional reference framework. This identified genes with conserved/divergent patterns, providing...
Although elevated body mass index (BMI) is a risk factor for cardiac disease, patients with BMI have better survival in the context of severe illness, phenomenon termed "obesity paradox."Higher associated lower mortality sudden arrest (SCA) survivors.Data were collected on 1433 post-SCA patients, discharged alive from hospitals University Pittsburgh Medical Center between 2002 and 2012. Of those, 1298 documented during hospitalization follow-up data constituted study cohort.In overall...
Temporal identity factors regulate competence of neural progenitors to generate specific cell types in a time-dependent manner, but how they operate remains poorly defined. In the developing mouse retina, Ikaros zinc-finger transcription factor Ikzf1 regulates production early-born types, except cone photoreceptors. this study we show that, during early stages retinal development, another family protein, Ikzf4, functions redundantly with photoreceptor production. Using CUT&RUN and functional...
The balance of contralateral and ipsilateral retinogeniculate projections is critical for binocular vision, but the transcriptional programs regulating this process remain ill defined. Here we show that Pou class homeobox protein POU3F1 expressed in nascent mouse retinal ganglion cells (cRGCs) not RGCs (iRGCs). Upon Pou3f1 inactivation, proportion cRGCs reduced favor iRGCs, leading to abnormal projection ratios at optic chiasm. Conversely, misexpression progenitors increases production...
It has been demonstrated for some cancers that the frequency of somatic oncogenic mutations may vary in ancestral populations. To determine whether key driver alterations might occur at different frequencies colorectal cancer, we applied a high-throughput genotyping platform (OncoMap) to query 385 across 33 known cancer genes DNA from 83 Asian, 149 Black and 195 White patients. We found Asian patients had fewer canonical tested (60% vs 79% (P = 0.011) 77% 0.015)), BRAF occurred higher (17%...
SUMMARY Developing mammalian brains are characterized by disproportionate growth of the forebrain compared to other regions. How this localized expansion occurs is, however, largely unknown. To address this, we identified region-specific neurogenic patterns creating a single-cell-resolution birthdate atlas mouse brain ( https://www.neurobirth.org ). We report that in regions, neurogenesis is sustained hindbrain, where transient and limited early development. Sustained reflects lengthened...
Purpose: To investigate the dosimetric accuracy of sliding window gated IMRT compared with static treatment, using varying dose rates.Materials and methods: This study measured changes in output diode array response changing rate, verified precision motion table, distribution film diodes at two depths rate.During 4DCT (4 Dimensional Computed Tomography), patient's respiratory signals target were recorded imported to XY4D simulation table SUN NUCLEAR Corporation simulate respiration tumour...
Current guidelines recommend implantable cardioverter-defibrillator (ICD) therapy in survivors of sudden cardiac arrest (SCA), except those with completely reversible causes. We sought to examine the impact ICD on mortality SCA associated causes.We evaluated records 1433 patients managed at our institution between 2000 and 2012 who were discharged alive after SCA. A correctable cause was identified 792 (55%) patients. Reversible defined as significant electrolyte or metabolic abnormality,...
Abstract Astrocytes represent one of the most abundant cell types in central nervous system, and play an essential role nearly all aspects brain functions 1 . Recent studies have challenged notion that cortical astrocytes are a uniform population, highlighted their diverse characteristics at morphological, molecular, functional levels 2-5 However, how this diversity originates establishes during development, remains largely unknown. Using single-cell RNA sequencing, we identified five...
African American (AA) colon cancer patients have a worse prognosis than Caucasian (CA) patients, however, reasons for this disparity are not well understood. To determine if tumor biology might contribute to differential prognosis, we measured recurrence risk and gene expression using the Oncotype DX® Colon Cancer Assay (12-gene assay) compared Recurrence Score results profiles between AA CA with stage II cancer. We retrieved demographic, clinical, archived tissues from at four institutions....
Many transcription factors regulating the production, survival, and function of photoreceptor cells have been identified, but little is known about transcriptional co-regulators in retinal health disease. Here, we show that BCL6 co-repressor (BCOR), a Polycomb repressive complex 1 factor mutated various cancers, involved degenerative diseases. Using proteomics assays, report BCOR interacts with CRX OTX2 reduces their ability to activate promoters photoreceptor-specific genes. CUT&RUN...
The electrocardiogram (ECG) is the most clinically accepted diagnostic tool used by physicians for interpreting functional activity of heart. existing ECG machines require an expert-in-the-loop identifying abnormalities in cardiac - commonly referred to as Arrhythmia a patient. accuracy diagnosis directly dependent on skill set physician; result, rural and remote places, where no specialist wants relocate, patients are unable get any help case life threatening arrhythmias. In this paper, we...
ABSTRACT Temporal identity factors regulate the competence of neural progenitors to generate specific cell types in a time-dependent manner, but how they operate remains poorly defined. In developing mouse retina, Ikaros zinc finger transcription factor Ikzf1 regulates production early-born types, except cone photoreceptors. this study we show that Ikzf4, another family protein, cooperates with control photoreceptor during early stages retinal development, whereas at late stages, when is no...