A5068481505- Single-cell and spatial transcriptomics
- Hypothalamic control of reproductive hormones
- Neural dynamics and brain function
- RNA Research and Splicing
- Plant Reproductive Biology
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Ovarian function and disorders
- Regulation of Appetite and Obesity
- Radio Frequency Integrated Circuit Design
- Ion channel regulation and function
- Advanced Power Amplifier Design
- Neuroscience and Neural Engineering
- Gastrointestinal motility and disorders
- Adenosine and Purinergic Signaling
- Pain Mechanisms and Treatments
- Nerve injury and regeneration
- Ecology and Vegetation Dynamics Studies
- Genetic diversity and population structure
- Biochemical Analysis and Sensing Techniques
- Genomics and Chromatin Dynamics
- Neurobiology and Insect Physiology Research
- Microstructure and mechanical properties
- Neuropeptides and Animal Physiology
Max Planck Institute of Biochemistry
2023-2024
Max Planck Institute of Neurobiology
2018-2024
Max Planck Institute for Biological Intelligence
2023-2024
Max Planck Institute for Intelligent Systems
2023
Ludwig-Maximilians-Universität München
1978-2022
Deutsche Telekom (Austria)
2020
NYU Langone Health
2015-2018
New York Genome Center
2017-2018
Harvard University
2017-2018
New York University
2018
Puberty onset is initiated by activation of neurons that secrete gonadotropin-releasing hormone (GnRH). The timing and progression puberty may depend upon temporal coordination two opposing central mechanisms—a restraint GnRH secretion before onset, followed enhanced stimulation release to complete reproductive maturation during puberty. Neuronal estrogen receptor α (ERα) has been implicated in both controls; however, the underlying neural circuits are not well understood. Here we test...
Abstract During neurogenesis, mitotic progenitor cells lining the ventricles of embryonic mouse brain undergo their final rounds cell division, giving rise to a wide spectrum postmitotic neurons and glia 1,2 . The link between developmental lineage cell-type diversity remains an open question. Here we used massively parallel tagging progenitors track clonal relationships transcriptomic signatures during forebrain development. We quantified divergence convergence across all major classes...
1. Electrical stimulation of interganglionic fiber tracts Auerbach's plexus released a chemical substance, which produced slow EPSP associated with an increase in input resistance the postsynaptic neuron. 2. This effect tract was mimicked by microiontophoretic application serotonin (5-HT) to neurons. Both endogenous transmitter substance and exogenous 5-HT membrane depolarization, both increased neuron, reduced or abolished hyperpolarizing afterpotentials action potentials, augmented...
1. Ganglion cells of the myentric plexus guinea‐pig small intestine were studied with intracellular recording methods. 2. Electrical stimulation interganglionic connectives elicited slow synaptic excitation (slow e.p.s.p.) that was associated an increase in input resistance cell. The e.p.s.p.s continued for several seconds after termination stimulation, and they occurred only neurones which prolonged hyperpolarizing after‐potentials followed action potential. 3. Superfusion solutions...
Sexual behavior is essential for the survival of many species. In female rodents, mate preference and copulatory depend on pheromones are synchronized with ovulation to ensure reproductive success. The neural circuits driving this orchestration in brain have, however, remained elusive. Here, we demonstrate that neurons controlling mammalian at core a branching circuit governing both behavior. We show male odors detected vomeronasal organ activate kisspeptin mice. Classical kisspeptin/Kiss1R...
Abstract Horses revolutionized human history with fast mobility 1 . However, the timeline between their domestication and widespread integration as a means of transport remains contentious 2–4 Here we assemble collection 475 ancient horse genomes to assess period when these animals were first reshaped by agency in Eurasia. We find that reproductive control modern domestic lineage emerged around 2200 bce , through close-kin mating shortened generation times. Reproductive following severe...
Cortical neurogenesis follows a simple lineage: apical radial glia cells (RGCs) generate basal progenitors, and these produce neurons. How this occurs in species with expanded germinal zones folded cortex, such as human, remains unclear. We used single-cell RNA sequencing from individual cortical ferret barcoded lineage tracking to determine the molecular diversity of progenitor their lineages. identified multiple RGC classes that initiate parallel lineages, converging onto common class...
Abstract The mammalian telencephalon contains distinct GABAergic projection neuron and interneuron types, originating in the germinal zone of embryonic basal ganglia. How genetic information determines cell types is unclear. Here we use a combination vivo CRISPR perturbation, lineage tracing ChIP–sequencing analyses show that transcription factor MEIS2 favors development neurons by binding enhancer regions projection-neuron-specific genes during mouse development. requires presence...
Kisspeptin-Gpr54 signaling is critical for the GnRH neuronal network controlling fertility. The present study reports on a kisspeptin (Kiss)-green fluorescent protein (GFP) mouse model enabling brain slice electrophysiological recordings to be made from Kiss neurons in arcuate nucleus (ARN) and rostral periventricular region of third ventricle (RP3V). Using dual immunofluorescence, approximately 90% GFP cells RP3V females, ARN both sexes, are shown authentic Kiss-synthesizing adult mice....
1. Intracellular recording revealed a population of myenteric neurons with electrical behavior that appeared to be equivalent the tonic-type mechanosensitive found in earlier studies utilized extracellular single units. 2. Electrical stimulation interganglionic fiber tracts evoked slowly rising excitatory postsynaptic potential (slow EPSP) was prolonged for several seconds after termination stimulus these cells. The slow EPSP associated increased input resistance and augmented excitability...
Abstract Aim The intricate puzzle‐like geography of the western Mediterranean is a product long‐term tectonic and orogenic events, supplemented by repeated climatic oscillations since Pliocene. It offers numerous vicariance events that may be invoked to explain speciation in amphibians. We test for plausibility two mutually exclusive Iberian–African hypotheses basal split within newts genus Pleurodeles : (1) disconnection Betic arch c. 14 Ma (2) end Messinian salinity crisis 5.33 Ma....
Abstract Density has been suggested to affect variation in extra‐pair paternity ( EPP ) avian mating systems, because increasing density promotes encounter rates and thus opportunities. However, the significance of affecting intra‐ interspecific comparisons remained controversial, with more support from intraspecific comparisons. Neither experimental nor empirical studies have consistently provided for hypothesis. Testing hypothesis is challenging measures may not necessarily reflect...
The neuropeptide kisspeptin is a potent stimulator of GnRH neurons and has been implicated as major regulator the hypothalamus-pituitary-gonadal axis. There are mainly two anatomically segregated populations that express in female hypothalamus: one anteroventral periventricular nucleus (AVPV) other arcuate (ARC). Distinct roles have proposed for AVPV ARC during reproductive maturation mediating estrogen feedback on axis adults. Despite their pivotal role regulation physiology, little known...
In August 2019, three dogs died after bathing in or drinking from Mandichosee, a mesotrophic reservoir of the River Lech (Germany). The showed symptoms neurotoxic poisoning and intoxication with cyanotoxins was considered. Surface blooms were not visible at time incidents. Benthic Tychonema sp., potential anatoxin-a (ATX)-producing cyanobacterium, detected mats growing on banks, as biofilm macrophytes later aggregations floating lake surface. dogs’ pathological examinations lung liver...
The central amygdala (CeA) consists of numerous genetically defined inhibitory neurons that control defensive and appetitive behaviors including feeding. Transcriptomic signatures cell types their links to function remain poorly understood. Using single-nucleus RNA sequencing, we describe nine CeA clusters, which four are mostly associated with two aversive behaviors. To analyze the activation mechanism neurons, characterized serotonin receptor 2a (Htr2a)-expressing (CeAHtr2a) comprise three...
Insulin in the brain plays an important role regulating reproductive function, as demonstrated via conditional brain-specific insulin receptor (Insr) deletion (knockout). However, specific neuronal target cells mediating central effects of on axis remain unidentified. We first investigated whether can act direct gonadotrophin-releasing hormone (GnRH) neurones. After clearly detecting Insr mRNA immunopurified GnRH cell fraction, we confirmed presence protein (InsR) approximately 82% neurones...
Species often occur in subdivided populations as a consequence of spatial heterogeneity the habitat. To describe organization subpopulations, existing theory proposes three main population models: patchy population, metapopulation and isolated populations. These models differ their predicted levels connectivity among risk that subpopulation will go extinct. However, spatially discrete subpopulations are commonly considered to be organized metapopulations, even though explicit tests...