A5100353352- Hearing, Cochlea, Tinnitus, Genetics
- Monoclonal and Polyclonal Antibodies Research
- Sleep and Wakefulness Research
- Glycosylation and Glycoproteins Research
- Circadian rhythm and melatonin
- Genetic Neurodegenerative Diseases
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Vestibular and auditory disorders
- Receptor Mechanisms and Signaling
- Advanced Radiotherapy Techniques
- Sleep and related disorders
- Head and Neck Cancer Studies
- Cardiac electrophysiology and arrhythmias
- T-cell and B-cell Immunology
- Effects of Radiation Exposure
- Cerebrospinal fluid and hydrocephalus
- Ear Surgery and Otitis Media
- Advanced MRI Techniques and Applications
- Ion Channels and Receptors
- Neurobiology and Insect Physiology Research
- Advanced Neuroimaging Techniques and Applications
- RNA and protein synthesis mechanisms
- Multimodal Machine Learning Applications
Johns Hopkins Medicine
2015-2025
Johns Hopkins University
2013-2025
Peking University
2010-2023
Eye & ENT Hospital of Fudan University
2023
State Key Laboratory of Medical Neurobiology
2023
Peking University Cancer Hospital
2023
Xinjiang University
2023
Soochow University
2023
Indiana University School of Medicine
2022
Jilin University
2022
Design and synthesis of basic functional circuits are the fundamental tasks synthetic biologists. Before it is possible to engineer higher‐order genetic networks that can perform complex functions, a toolkit devices must be developed. Among those devices, sequential logic expected foundation information‐processing systems. In this study, we report design construction circuit in Escherichia coli . It generate different outputs response same input signal on basis its internal state, ‘memorize’...
Significance Painfully loud sound causes protective or withdrawal responses, rather than continued listening. This differential behavior invites comparison with somatic pain responses driven by the anatomically distinct subset of small-diameter, unmyelinated afferents—C fibers. Like C fibers, type II cochlear afferents differ in size, number, and innervation pattern from I that encode sound. Here, we show are excited during tissue damage part algogenic cytoplasmic metabolite adenosine...
Blood-brain barrier (BBB) plays a critical role in protecting the brain from toxins and pathogens. However, vivo tools to assess BBB permeability are scarce often require use of exogenous contrast agents. In this study, we aimed develop non-contrast arterial-spin-labeling (ASL) based MRI technique estimate water mice. By determining relative fraction labeled spins that were exchanged into tissue as opposed those remained cerebral veins, estimated indices global including extraction (E)...
The distinctive planar polarity of auditory hair cells is evident in the polarized organization stereociliary bundle. Mutations core cell gene Van Gogh-like 2 (<i>Vangl2</i>) result that fail to properly orient their bundles along mediolateral axis cochlea. severity this phenotype graded length cochlea, similar differentiation gradient, suggesting an active refinement process corrects phenotypes <i>Vangl2</i> knock-out (KO) mice. Because deletions are lethal, conditional knock-outs (CKOs)...
The accumulation of mutant huntingtin protein aggregates in neurons is a pathological hallmark Huntington's disease (HD). glymphatic system, brain-wide perivascular network, facilitates the exchange interstitial fluid (ISF) and cerebrospinal (CSF), supporting solute clearance brain wastes. In this study, we employed dynamic glucose-enhanced (DGE) MRI to measure D-glucose from CSF as tool predict function mouse model HD. We found significantly diminished efficiency HD mice prior phenotypic...
Abstract Mechanosensory hair cells release glutamate at ribbon synapses to excite postsynaptic afferent neurons, via AMPA-type ionotropic receptors (AMPARs). However, type II neurons contacting outer in the mammalian cochlea were thought differ this respect, failing show GluA immunolabeling and with many “ribbonless” contacts. Here it is shown that antibodies AMPAR subunit GluA2 labeled contacts below inner rat cochlea, synaptic currents afferents had AMPAR-specific pharmacology. Only half...
Huntington's disease (HD) is a progressive neurodegenerative disorder caused by CAG-repeat expansion in exon-1 of the huntingtin gene. Currently, no disease-modifying therapies are available, with significant challenge evaluating therapeutic efficacy before clinical symptoms emerge. This highlights need for early biomarkers and intervention strategies. Therefore, it essential to develop characterize accurate mouse models identify preclinical development. In this study, we characterized...
Significance Subtype-selective modulation of ion channels is often important, but extremely difficult to achieve for drug development. Using Nav1.7 as an example, we show that this challenge could be attributed poor design in channel assays, which fail detect most potent and selective compounds are biased toward nonselective mechanisms. By exploiting different binding sites modes gating, successfully direct a membrane potential assay non–pore-blocking mechanisms identify Nav1.7-selective...
The transient receptor potential ankyrin 1 (TRPA1) channel functions as an irritant sensor and is a therapeutic target for treating pain, itch, respiratory diseases. As ligand-gated channel, TRPA1 can be activated by electrophilic compounds such allyl isothiocyanate (AITC) through covalent modification or noncovalent agonists ligand binding. However, how leads to opening and, importantly, binding activates are not well-understood. Here we report class of piperidine carboxamides (PIPCs)...
Due to their important physiological functions, ion channels are key therapeutic targets for a variety of disorders. However, electrophysiological assessment channel activity is technically challenging and has been bottleneck in the discovery drugs that modulate function. To address this issue, automated patch clamp platforms have developed with improved throughput broader applications. An overview current status high-throughput electrophysiology its applications drug provided. © 2019 The Authors.
Ocular vascular diseases are the major causes of visual impairment, which characterized by retinal dysfunction and robust inflammatory responses. Traditional anti-angiogenic or anti-inflammatory drugs still have limitations due to short-acting effects. To improve efficiency, a dual-drug nanocomposite formulation was proposed for combined treatment ocular diseases.CBC-MCC@hMSN(SM) complex nanoformulation prepared integrating conbercept (CBC, an drug) MCC950 (MCC, inhibitor inflammation) into...
Cisplatin is one of the most widely used chemotherapeutic drugs across world. However, serious ototoxic effects, leading to permanent hair cell death and hearing loss, significantly limit utility cisplatin. In zebrafish, functional mechanotransduction channel required for cisplatin ototoxicity. it still unclear extent which involved in uptake ototoxicity mammalian cells. Herein, we show that genetically disrupting mouse partially protects cells from cisplatin-induced death. Using a...
ABSTRACT Huntington’s disease (HD) is a neurodegenerative disorder that presents with progressive motor, mental, and cognitive impairment leading to early disability mortality. The accumulation of mutant huntingtin protein aggregates in neurons pathological hallmark HD. glymphatic system, brain-wide perivascular network, facilitates the exchange interstitial fluid (ISF) cerebrospinal (CSF), supporting solute clearance including abnormal proteins from mammalian brains. In this study, we...
Epoxyeicosatrienoic acids (EETs), angiogenic mediators degraded by soluble epoxide hydrolase (sEH), have been shown to exert beneficial effects on the cardiovascular system. The current study assessed impact of increased EETs with an sEH inhibitor, t-AUCB, two-kidney-one-clip (2K1C)-induced renovascular endothelial dysfunction, associated hypertension, in rats. hypertensive rats exhibited systolic blood pressure, reduced renal flow, impaired endothelium-dependent relaxation and eNOS...
Hearing loss (HL) is a common sensory disorder. More than half of HL cases can be attributed to genetic causes. There no effective therapy for at present, early diagnosis reduce the incidence important clinical intervention in HL. Previous studies have identified 111 nonsyndromic hearing genes. The most frequently mutated genes NSHL patients China include GJB2, SLC26A4, and mitochondrial gene MT-RNR1. It develop panels Chinese population, which allow etiologic both SHL NSHL. In this study,...
Sialic acid (SA) is crucial for protecting glycoproteins from clearance. Efmarodocokin alfa (IL-22Fc), a fusion protein agonist that links IL-22 to the crystallizable fragment (Fc) of human IgG4, contains 8 N-glycosylation sites and exhibits heterogeneous variable terminal sialylation biodistribution. This presents unique challenge Pharmacokinetic (PK) Pharmacodynamic (PD) analysis cross-species translation. In this study, we sought understand how varying SA levels distribution contribute...
In this work we formulate the problem of image captioning as a multimodal translation task. Analogous to machine translation, present sequence-to-sequence recurrent neural networks (RNN) model for caption generation. Different from most existing where whole is represented by convolutional network (CNN) feature, propose represent input sequence detected objects which feeds source RNN model. way, sequential representation an can be naturally translated words, target To in extract features and...
The objective of this study was to evaluate the anatomical changes and associated dosimetric consequences pharyngeal constrictor muscles (PCMs) that occur during head neck (H N) radiotherapy (RT).A cohort 13 oropharyngeal cancer patients with daily cone beam computed tomography (CBCT) retrospectively studied. On every 5th CBCT image, PCM manually delineated by a radiation oncologist. anterior-posterior thickness measured at midline level C3 vertebral body. Delivered dose estimated...