A5100732236- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Autophagy in Disease and Therapy
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Skin and Cellular Biology Research
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Vector-Borne Animal Diseases
- 14-3-3 protein interactions
- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Laser-Matter Interactions and Applications
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Silk-based biomaterials and applications
- Plant Reproductive Biology
- Neuroscience and Neuropharmacology Research
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Osteoarthritis Treatment and Mechanisms
- Monoclonal and Polyclonal Antibodies Research
Huashan Hospital
2025
Fudan University
2025
China Aerospace Science and Technology Corporation
2025
Peking University
2015-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2021-2024
Chongqing Medical University
2024
Children's Hospital of Chongqing Medical University
2024
Huazhong Agricultural University
2024
China Electronics Technology Group Corporation
2023
China Institute of Water Resources and Hydropower Research
2023
Impurity doping often alters or improves the properties of materials. In alumina, grain boundaries play a key role in deformation mechanisms, particularly phenomenon boundary sliding during creep at high temperatures. We elucidated atomic-scale structure alumina and its relationship to suppression upon with yttrium by using atomic resolution microscopy high-precision calculations. find that segregates very localized regions along local bonding environment, thereby strengthening against...
The 3C-like proteinase of severe acute respiratory syndrome (SARS) coronavirus has been proposed to be a key target for structural-based drug design against SARS. In order understand the active form and substrate specificity enzyme, we have cloned, expressed, purified SARS proteinase. Analytic gel filtration shows mixture monomer dimer at protein concentration 4 mg/ml mostly 0.2 mg/ml, which correspond used in enzyme assays. linear decrease enzymatic-specific activity with revealed that only...
MAP1B and MAP2 are major members of neuronal microtubule-associated proteins (MAPs). To gain insights into the function in vivo, we generated MAP2-deficient (map2−/−) mice. They developed without any apparent abnormalities, which indicates that is dispensable mouse survival. Because previous reports suggest a functional redundancy among MAPs, next mice lacking both to test their possible synergistic functions vivo. Map2−/−map1b−/− died perinatal period. showed not only fiber tract...
Design and synthesis of basic functional circuits are the fundamental tasks synthetic biologists. Before it is possible to engineer higher‐order genetic networks that can perform complex functions, a toolkit devices must be developed. Among those devices, sequential logic expected foundation information‐processing systems. In this study, we report design construction circuit in Escherichia coli . It generate different outputs response same input signal on basis its internal state, ‘memorize’...
Astrocyte activation is a hallmark of central nervous system injuries resulting in glial scar formation (astrogliosis). The astrocytes involves metabolic and morphological changes with complex underlying mechanisms, which should be defined to provide targets for astrogliosis intervention. Astrogliosis usually accompanied by an upregulation fibrillary acidic protein (GFAP). Using vitro scratch injury model, we scratched primary cultures cerebral cortical observed influx calcium the form waves...
The endoplasmic reticulum (ER) is composed of the nuclear envelope, perinuclear sheets and a peripheral tubular network. ER mitochondria form tight contacts at specific subdomains, which coordinate functions two organelles are required for multiple cellular processes such as Ca2+ transfer apoptosis. However, it largely unknown how morphology ER-mitochondria signaling dynamically regulated under different physiological or pathological conditions DNA damage. Here we show that peripheral,...
During interphase, centrosomes are connected by a proteinaceous linker between the proximal ends of centrioles, which is important for to function as single microtubule-organizing center. However, composition and regulation centrosomal remain largely unknown. Here, we show that LRRC45 centrosome localizes at centrioles forms fiber-like structures them. Depletion results in splitting during interphase. Moreover, interacts with both C-Nap1 rootletin phosphorylated Nek2A S661 mitosis. After...
Disturbance of endoplasmic reticulum (ER) homeostasis causes ER stress and leads to activation the unfolded protein response, which reduces promotes cell survival at early stage stress, or triggers death apoptosis when is not restored under prolonged stress. Here, we report that Cab45S, a member CREC family, inhibits stress-induced apoptosis. Depletion Cab45S increases inositol-requiring kinase 1 (IRE1) activity, thus producing more spliced forms X-box-binding mRNA phosphorylation c-Jun...
Abstract The primary cilium is elongated from the mother centriole and has diverse signaling roles during development disease. CP110-CEP97 complex functions as a negative regulator of ciliogenesis, although mechanisms regulating its localization are poorly understood. Here we show that M-Phase Phosphoprotein 9 (MPP9) recruited by Kinesin Family Member 24 (KIF24) to distal end where it forms ring-like structure recruits directly binding CEP97. Loss MPP9 causes abnormal cilia formation in...
Abstract In animal cells, the centrosome is main microtubule-organizing centre where microtubules are nucleated and anchored. The centriole subdistal appendages (SDAs) key structures that anchor in interphase but composition assembly mechanisms of SDAs not well understood. Here, we reveal centrosome-binding proteins, coiled-coil domain containing (CCDC) 120 CCDC68 two novel SDA components required for hierarchical human cells. CCDC120 anchored to by ODF2 recruits CEP170 Ninein through...
Significance AMPA receptors (AMPARs) are major postsynaptic that mediate fast excitatory neurotransmission and synaptic plasticity. The proper functioning of AMPARs is essential for brain function; AMPAR dysfunction can cause multiple neurologic disorders, including autism. Native macromolecular complexes associated with a variety auxiliary proteins, α/β-hydrolase domain-containing 6 (ABHD6), which was recently identified. However, the physiological significance ABHD6–AMPAR association has...
Abstract Three diazafluorene derivatives triphenylamine (TPA)(PDAF) n ( = 1, 2, 3) serving as small molecular elements are designed and synthesized via concentrated sulfuric acid mediated Friedel–Crafts reaction. With highly nonplanar topological configuration, TPA(PDAF) 3 shows weaker intermolecular interaction in the solid states thus exhibits single nanomolecular behavior, which is crucial for charge stored retained an organic field‐effect transistor (OFET) memory device. Furthermore,...
The ER tethers tightly to mitochondria and the mitochondrial protein FUNDC1 recruits Drp1 ER-mitochondria contact sites, subsequently facilitating fission preventing from undergoing hypoxic stress. However, mechanisms by which modulates hypoxia-induced are poorly understood. Here, we show that USP19, an ER-resident deubiquitinase, accumulates at sites under hypoxia promotes division. In response hypoxia, USP19 binds deubiquitinates facilitates oligomerization GTP-binding hydrolysis...
Neurofilaments are the major cytoskeletal elements in axon that take highly ordered structures composed of parallel arrays 10-nm filaments linked to each other with frequent cross-bridges, and they believed maintain a polarized neuronal cell shape. Here we report function rat NF-M this characteristic neurofilament assembly. Transfection experiments were done an insect Sf9 line lacking endogenous intermediate filaments. NF-L coassemble form bundles packed manner cross-bridges resembling...
Endoplasmic reticulum (ER) stress is caused by the disturbance of ER homeostasis and leads to activation unfolded protein response (UPR), which alleviates at an early stage triggers apoptosis if fails over a prolonged timeframe. Here, we report that reticulocalbin 1 (RCN1), member CREC family, transactivated nuclear factor kappa B (NF-κB) during inhibits stress-induced apoptosis. The depletion RCN1 increases UPR drug-induced activating PRKR-like kinase-CCAAT/enhancer-binding...
Decreasing the position error and control torque is important for coordinate of a modular unmanned system with less communication burden between sensor actuator. Therefore, this paper proposes event-trigger reinforcement learning (ETRL)-based (MUS) via nonzero-sum game (NZSG) strategy. The dynamic model MUS established joint feedback (JTF) technology. Based on NZSG strategy, existing problem transformed into an RL issue. With help ET mechanism, periodic mechanism avoided. ET-critic neural...