A5014095048- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Mathematical and Theoretical Epidemiology and Ecology Models
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Cardiovascular Function and Risk Factors
- Inflammasome and immune disorders
- Cardiac Ischemia and Reperfusion
- Estrogen and related hormone effects
- Nitric Oxide and Endothelin Effects
- Spinal Cord Injury Research
- COVID-19 epidemiological studies
- Adipose Tissue and Metabolism
- Retinoids in leukemia and cellular processes
- Cell death mechanisms and regulation
- Evolution and Genetic Dynamics
- CRISPR and Genetic Engineering
- Fractional Differential Equations Solutions
- ATP Synthase and ATPases Research
- Nuclear Receptors and Signaling
- Cancer, Hypoxia, and Metabolism
- Receptor Mechanisms and Signaling
- Genetics and Neurodevelopmental Disorders
- Peroxisome Proliferator-Activated Receptors
- Signaling Pathways in Disease
Peking University
2016-2025
Capital Medical University
2011-2024
Jilin Agricultural University
2023-2024
Shanghai Pulmonary Hospital
2024
Tongji University
2024
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2020-2023
Wenzhou Medical University
2005-2023
National Clinical Research Center for Digestive Diseases
2022-2023
Xi'an Polytechnic University
2007-2022
State Key Laboratory of Natural and Biomimetic Drugs
2022
Spinal cord injury (SCI) results in a wide range of disabilities.Its complex pathophysiological process limits the effectiveness many clinical treatments.Betulinic acid (BA) has been shown to be an effective treatment for some neurological diseases, but it not studied SCI.In this study, we assessed role BA SCI and investigated its underlying mechanism.We used mouse model SCI, functional outcomes following were assessed.Western blotting, ELISA, immunofluorescence techniques employed analyze...
Background: CaMKII (Ca 2+ /calmodulin-dependent kinase II) plays a central role in cardiac ischemia/reperfusion (I/R) injury—an important therapeutic target for ischemic heart disease. In the heart, CaMKII-δ is predominant isoform and further alternatively spliced into 11 variants. humans, CaMKII-δ9 CaMKII-δ3, major splice variants, inversely regulate cardiomyocyte viability with former pro-death latter pro-survival. However, it unknown whether specific inhibition of detrimental prevents I/R...
Abstract Cachexia affects 50–80% of patients with cancer and accounts for 20% cancer-related death, but the underlying mechanism driving cachexia remains elusive. Here we show that circulating lactate levels positively correlate degree body weight loss in male female suffering from cachexia, as well clinically relevant mouse models. Lactate infusion per se is sufficient to trigger a cachectic phenotype tumour-free mice dose-dependent manner. Furthermore, demonstrate adipose-specific...
AMP activated protein kinase (AMPK) plays an important role in regulating myocardial metabolism and synthesis. Activation of AMPK attenuates hypertrophy cultured cardiac myocytes, but the development response to chronic pressure overload is not known. To test hypothesis that AMPKalpha2 protects heart against systolic overload-induced ventricular dysfunction, we studied gene deficient (knockout [KO]) mice wild-type subjected 3 weeks transverse aortic constriction (TAC). Although KO had no...
Objective— The objective of this study was to identify the role dimethylarginine dimethylaminohydrolase-1 (DDAH1) in degrading endogenous nitric oxide synthase inhibitors asymmetrical (ADMA) and N g -monomethyl- l -arginine ( -NMMA). Methods Results— We generated a global-DDAH1 gene–deficient (DDAH1 −/− ) mouse strain examine DDAH1 ADMA -NMMA degradation physiological consequences loss DDAH1. Plasma tissue levels mice were several folds higher than wild-type mice, but growth development...
Diabetic cardiomyopathy, which contributes to >50% diabetic death, is featured by myocardial lipid accumulation, hypertrophy, fibrosis, and cardiac dysfunction. The mechanism underlying cardiomyopathy poorly understood. Recent studies have shown that a striated muscle-specific E3 ligase Mitsugumin 53 (MG53, or TRIM72) constitutes primary causal factor of systemic insulin resistance metabolic disorders. Although it most abundantly expressed in myocardium, the biological pathological roles...
Mitsugumin 53 (MG53 or TRIM72), a striated muscle-specific E3 ligase, promotes ubiquitin-dependent degradation of the insulin receptor and substrate-1 subsequently induces resistance, resulting in metabolic syndrome type 2 diabetes mellitus (T2DM). However, it is unknown how MG53 from muscle regulates systemic response energy metabolism. Increasing evidence demonstrates that secretes proteins as myokines cardiokines regulate processes. We hypothesize may act myokine/cardiokine, contributing...
The molecular mechanism controlling the zygotic genome activation (ZGA) in mammals remains poorly understood. 2-cell (2C)-like cells spontaneously emerging from cultures of mouse embryonic stem (ESCs) share some key transcriptional and epigenetic programs with 2C-stage embryos. By studying transition ESCs into 2C-like cells, we identified developmental pluripotency associated 2 4 (Dppa2/4) as important regulators program through directly up-regulating expression double homeobox (Dux). In...
The impairment of mitochondrial bioenergetics, often coupled with exaggerated reactive oxygen species (ROS) production, is a fundamental disease mechanism in organs high demand for energy, including the heart. Building more robust and safer cellular powerhouse holds promise protecting these stressful conditions. Here, we demonstrate that NADH:ubiquinone oxidoreductase subunit AB1 (NDUFAB1), also known as acyl carrier protein, acts powerful cardio-protector by conferring greater capacity...
Cardiac ischemia/reperfusion (I/R) injury has emerged as an important therapeutic target for ischemic heart disease, the leading cause of morbidity and mortality worldwide. At present, there is no effective therapy reducing cardiac I/R injury. CaMKII (Ca2+/calmodulin-dependent kinase II) plays a pivotal role in pathogenesis severe conditions, including Pharmacological inhibition strategy protection against myocardial damage diseases. To date, drug targeting clinical disease. Furthermore, at...
Abstract Intervertebral disc degeneration (IVDD) is a chronic age-related degenerative disease accompanied by complex pathophysiological mechanisms. Increasing evidence indicates that NLRP3 inflammasome mediated pyroptosis of nucleus pulposus (NP) cells displays an important role in the pathological progression IVDD. Milk fat globule-EGF factor-8 (MFG-E8) endogenously secreted glycoprotein with beneficial effects anti-inflammatory, antioxidant, and modulation inflammasome. However, effect...
Necroptosis and pyroptosis, two types of proinflammatory programmed cell death, were recently found to play important roles in spinal cord injury (SCI). Moreover, cyclic helix B peptide (CHBP) was designed maintain erythropoietin (EPO) activity protect tissue against the adverse effects EPO. However, protective mechanism CHBP following SCI is still unknown. This research explored necroptosis- pyroptosis-related underlying neuroprotective effect after SCI. Gene Expression Omnibus (GEO)...
Receptor-interacting protein 140 (RIP140) encodes a histone deacetylase (HDAC) inhibitor-sensitive repressive activity. Direct interaction of RIP140 with HDAC1 and HDAC3 occurs <i>in vitro</i> vivo</i> as demonstrated in co-immunoprecipitation glutathione <i>S</i>-transferase pull-down experiments. The HDAC-interacting domain is mapped to its N-terminal domain, between amino acids 78 303 based upon In chromatin immunoprecipitation assays, it that deacetylation at the region Gal4 binding...
Inducible nitric oxide synthase (iNOS) protein is expressed in cardiac myocytes of patients and experimental animals with congestive heart failure (CHF). Here we show that iNOS expression plays a role pressure overload-induced myocardial chamber dilation hypertrophy. In wild-type mice, chronic transverse aortic constriction (TAC) resulted expression, hypertrophy, ventricular dysfunction, fibrosis, whereas iNOS-deficient mice displayed much less dilation, dysfunction. Consistent these...
Extracellular superoxide dismutase (SOD) contributes only a small fraction to total SOD activity in the normal heart but is strategically located scavenge free radicals extracellular compartment. To examine physiological significance of response hemodynamic stress, we studied effect deficiency on transverse aortic constriction (TAC)-induced left ventricular remodeling. Under unstressed conditions had no myocardial activity, ratio glutathione:glutathione disulfide, nitrotyrosine content, or...
The normal expression of myocardial mitochondrial enzymes is essential to maintain the cardiac energy reserve and facilitate responses stress, but molecular mechanisms enzyme have been elusive. Here we report that congestive heart failure associated with a significant decrease estrogen-related receptor-α (ERRα), not peroxisome proliferator-activated receptor-γ coactivator 1α, in human samples. In addition, chronic pressure overload mice caused ERRα was significantly correlated degree left...
Activation of AMP-activated protein kinase (AMPK)-α2 protects the heart against pressure overload–induced failure in mice. Although metformin is a known activator AMPK, it unclear whether its cardioprotection acts independently an AMPKα2-dependent pathway. Because role AMPKα1 stimulation on remodeling failing hearts poorly defined, we first studied effects disruption both and AMPKα2 genes response to transverse aortic constriction–induced left ventricular (LV) hypertrophy dysfunction gene...
Rationale: We hypothesized that the differentiation processes of cardiac progenitor cell (CP) from first and second heart fields (FHF SHF) may undergo unique instructive gene regulatory networks or signaling pathways, precise SHF progression is contingent on FHF developmental cues. Objective: investigated how intraorgan communications control sequential building discrete anatomic regions at single-cell resolution. Methods Results: By transcriptomic analysis Nkx2-5 (NK2 homeobox 5) Isl1 (ISL...