A5074846688- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- NF-κB Signaling Pathways
- Viral Infections and Immunology Research
- Stock Market Forecasting Methods
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- 3D Printing in Biomedical Research
- Signaling Pathways in Disease
- Gene Regulatory Network Analysis
- Plant Disease Resistance and Genetics
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Energy Load and Power Forecasting
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Genetic Mapping and Diversity in Plants and Animals
- Anesthesia and Neurotoxicity Research
- Forecasting Techniques and Applications
- Connective tissue disorders research
Shanghai Cell Therapy Research Institute
2022-2023
Jinan University
2021-2023
Southern University of Science and Technology
2022-2023
Nanfang Hospital
2023
Sun Yat-sen University
2023
Cell Technology (China)
2021-2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2022
Beijing Anzhen Hospital
2016-2021
Capital Medical University
2016-2021
Beijing Institute of Water
2016-2021
Background: CaMKII (Ca 2+ /calmodulin-dependent kinase II) plays a central role in cardiac ischemia/reperfusion (I/R) injury—an important therapeutic target for ischemic heart disease. In the heart, CaMKII-δ is predominant isoform and further alternatively spliced into 11 variants. humans, CaMKII-δ9 CaMKII-δ3, major splice variants, inversely regulate cardiomyocyte viability with former pro-death latter pro-survival. However, it unknown whether specific inhibition of detrimental prevents I/R...
Cardiac ischemia/reperfusion (I/R) injury has emerged as an important therapeutic target for ischemic heart disease, the leading cause of morbidity and mortality worldwide. At present, there is no effective therapy reducing cardiac I/R injury. CaMKII (Ca2+/calmodulin-dependent kinase II) plays a pivotal role in pathogenesis severe conditions, including Pharmacological inhibition strategy protection against myocardial damage diseases. To date, drug targeting clinical disease. Furthermore, at...
Simple sequence repeats (SSRs) have been widely used in maize genetics and breeding, because they are co-dominant, easy to score, highly abundant. In this study, we whole-genome sequences from 16 inbreds 1 wild relative determine SSR abundance develop a set of high-density polymorphic markers. A total 264 658 SSRs were identified across the 17 genomes, with an average 135 693 per genome. Marker density was one every 15.48 kb. (C/G)n, (AT)n, (CAG/CTG)n, (AAAT/ATTT)n most frequent motifs for...
Exercise-induced physiological hypertrophy provides protection against cardiovascular disease, whereas disease-induced pathological leads to heart failure. Emerging evidence suggests pleiotropic roles of melatonin in cardiac disease; however, the effects on vs remain unknown. Using swimming-induced and pressure overload-induced models, we found that treatment significantly improved hypertrophic responses accompanied by alleviated oxidative stress myocardium but did not affect levels. As an...
Abstract The traditional Chinese herb Lonicerae Japonicae Flos has shown significant clinical benefits in the treatment of heart failure, but mechanism remains unclear. As main active ingredient found plasma after oral administration , chlorogenic acid (CGA) been reported to possess anti‐inflammatory, anti‐oxidant and anti‐apoptosis function. We firstly confirmed cardioprotective effects CGA transverse aortic constriction (TAC)‐induced failure mouse model, through mitigating TNF‐α–induced...
Abstract Muscle LIM protein (MLP, CSRP3 ) is a key regulator of striated muscle function, and its mutations can lead to both hypertrophic cardiomyopathy (HCM) dilated (DCM) in patients. However, due lack human models, mechanisms underlining the pathogenesis MLP defects remain unclear. In this study, we generated knockout MLP/CSRP3 embryonic stem cell (hESC) H9 line using CRISPR/Cas9 mediated gene disruption. disruption had no impact on cardiac differentiation cells led confirmed deficiency...
Ischemic heart disease is the leading cause of morbidity and mortality worldwide. preconditioning (IPC) most powerful intrinsic protection against cardiac ischemia/reperfusion injury. Previous studies have shown that a multifunctional TRIM family protein, MG53 (mitsugumin 53; also called TRIM72), not only plays an essential role in IPC-mediated cardioprotection injury but ameliorates mechanical damage. In addition to its intracellular actions, as myokine/cardiokine, can be secreted from...
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Doxorubicin (DOX) is widely used to treat various cancers affecting adults and children; however, its clinical application limited by cardiotoxicity. Previous studies have shown that children are more susceptible the cardiotoxic effects of DOX than adults, which may be related different maturity levels cardiomyocyte, but underlying mechanisms not fully understood. Moreover, researchers investigating DOX-induced cardiotoxicity caused human-induced pluripotent stem cell-derived cardiomyocytes...
Human pluripotent stem cells (hPSCs) are a powerful platform for disease modeling and drug discovery. However, the introduction of known pathogenic mutations into hPSCs is time-consuming labor-intensive process. Base editing newly developed technology that enables facile point specific loci within genome living cells. Here, we design an all-in-one episomal vector expresses single guide RNA (sgRNA) with adenine base editor (ABE) or cytosine (CBE). Both ABE CBE can efficiently introduce cells,...
Induction of endogenous cardiomyocyte (CM) proliferation is one the key strategies for heart regeneration. Increasing evidence points to potential role microRNAs (miRNAs) in regulation CM proliferation. Here, we used human embryonic stem cell (hESC)-derived CMs (hESC-CMs) as a tool identify miRNAs that promote We profiled miRNA expression at an early stage differentiation and identified list highly expressed miRNAs. Among these miRNAs, miR-25 was enriched early-stage hESC-CMs, but its...
In this study, we assessed whether the down-regulation of Yes-associated protein (YAP) is involved in pathogenesis extracellular matrix (ECM) mechanical stress-induced Stanford type A aortic dissection (STAAD). Human samples were obtained from heart transplantation donors as normal controls and STAAD patients undergoing surgical replacement ascending aorta. Decreased maximum wall velocity, ECM disorders, increased VSMC apoptosis, YAP identified samples. a mouse model STAAD, was...
Spinal cord injury (SCI) is a neurological, medically incurable disorder. Human pluripotent stem cells (hPSCs) have the potential to generate neural stem/progenitor (NS/PCs), which hold promise in treatment of SCI by transplantation. In our study, we aimed establish chemically defined culture system using serum-free medium and ascorbic acid (AA) expand long-term self-renewing neuroepithelial-like (lt-NES cells) differentiated from hPSCs effectively stably. We induced human embryonic...
Adult mammalian cardiomyocytes (CMs) retain a limited proliferative ability, which is insufficient for the repair of CM loss in ischemic cardiac injury. Regulation Hippo signaling pathway to promote endogenous proliferation has emerged as promising strategy heart regeneration. Previous studies have shown that microRNA cluster miR302–367 negatively regulates pathway, promoting proliferation. In this study, we identified another microRNA, miR-10b, and promotes cell human embryonic stem...
Mutations in Junctophilin-2(JPH2) gene is the cause of hypertrophic cardiomyopathy (HCM) and leading inherited left ventricular hypertrophy myofilaments disarray. JPH2 protein, a member Junctophilin family, mainly expressed heart plays an important role E-C coupling. We have generated homozygous knockout (JPH2-KO) human embryonic stem cell (hESC) line using episomal vector-based CRISPR/Cas9 system. This JPH2-KO hESC maintained like morphology, pluripotency, normal karyotype could...
Ras associated with diabetes (RAD) is a membrane protein that acts as calcium channel regulator by interacting cardiac L-type Ca2 + channels (LTCC). RAD defects can disrupt intracellular dynamics and lead to hypertrophy. However, due the lack of reliable human disease models, pathological mechanism deficiency leading hypertrophy not well understood. In this study, we created RRAD -/- H9 cell line using CRISPR/Cas9 technology. disruption did affect ability efficiency cardiomyocytes...
Abstract Background Long-QT syndrome type 2 (LQT2) is a common malignant hereditary arrhythmia. Due to the lack of suitable animal and human models, pathogenesis LQT2 caused by ether-a-go-go-related gene (hERG) deficiency still unclear. In this study, we generated an hERG-deficient cardiomyocyte (CM) model that simulates ‘human homozygous hERG mutations’ explore underlying impact dysfunction genotype–phenotype relationship deficiency. Methods The KCNH2 was knocked out in embryonic stem cell...
The use of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is limited in drug discovery and cardiac disease mechanism studies due to cell immaturity. Although many approaches have been reported improve the maturation hiPSC-CMs, elucidation process crucial. We applied a small-molecule-based differentiation method generate (CMs) with multiple aggregation forms. motion analysis revealed significant physical differences differently shaped CMs, net-shaped CMs had larger...
Cyclophosphamide (CYP)-induced cardiotoxicity is a common side effect of cancer treatment. Although it has received significant attention, the related mechanisms CYP-induced remain largely unknown. In this study, we used cell and animal models to investigate CYP on cardiomyocytes. Our data demonstrated that prolonged cardiac QT interval electromechanical coupling time courses accompanied by JPH2 downregulation. Moreover, N6-methyladenosine (m6A) methylation sequencing RNA suggested induced...
Abstract Background Pseudoenzymes, catalytically deficient variants of active enzymes, have a wide range regulatory functions. ADP-ribosylhydrolase-like 1 (ADPRHL1), pseudoenzyme belonging to small group ADP-ribosylhydrolase enzymes that lacks the amino acid residues necessary for catalytic activity, may significant role in heart development based on accumulating evidence. However, specific function ADPRHL1 this process has not been elucidated. To investigate heart, we generated first vitro...