A5101903768- Genetic Mapping and Diversity in Plants and Animals
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Molecular Biology Techniques and Applications
- Plant Genetic and Mutation Studies
- Cancer-related molecular mechanisms research
- Genetics and Plant Breeding
- Crop Yield and Soil Fertility
- Soybean genetics and cultivation
- Plant Disease Resistance and Genetics
- Rice Cultivation and Yield Improvement
- Biofuel production and bioconversion
- Plant tissue culture and regeneration
- Mycobacterium research and diagnosis
- Wheat and Barley Genetics and Pathology
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Plant Stress Responses and Tolerance
- Genetic diversity and population structure
- CRISPR and Genetic Engineering
- Research in Cotton Cultivation
- Single-cell and spatial transcriptomics
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
Sichuan Agricultural University
2015-2024
Luye Pharma (China)
2019
Potato Research Institute
2017
Swami Keshwanand Rajasthan Agricultural University
2014
Sichuan Normal University
2013
Wuhan University
2005-2008
This paper describes two joint linkage–linkage disequilibrium (LD) mapping approaches: parallel (independent linkage and LD analysis) integrated (datasets analyzed in combination). These approaches were achieved using 2,052 single nucleotide polymorphism (SNP) markers, including 659 SNPs developed from drought-response candidate genes, screened across three recombinant inbred line (RIL) populations 305 diverse lines, with anthesis-silking interval (ASI), an important trait for maize drought...
The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a powerful and widely used technique for the measurement of gene expression. Reference genes, which serve as endogenous controls ensure that results are accurate reproducible, vital data normalization. To bolster literature on reference selection in maize, ten candidate including eight traditionally internal control genes two potential from our microarray datasets, were evaluated expression level maize across...
Drought stress is one of the major limiting factors for maize production. With availability B73 reference genome and whole-genome resequencing 15 inbreds, common variants (CV) clustering analyses were applied to identify non-synonymous SNPs (nsSNPs) corresponding candidate genes drought tolerance. A total 524 nsSNPs that associated with 271 involved in plant hormone regulation, carbohydrate sugar metabolism, signaling molecules redox reaction acclimation photosynthesis environment detected...
Understanding of genetic diversity and linkage disequilibrium (LD) decay in diverse maize germplasm is fundamentally important for improvement. A total 287 tropical 160 temperate inbred lines were genotyped with 1943 single nucleotide polymorphism (SNP) markers high quality compared LD using the SNPs their haplotypes developed from genic intergenic regions. Intronic revealed a substantial higher variation than exonic SNPs. The big window size (3-SNP slide-window covering 2160 kb on average)...
Simple sequence repeats (SSRs) have been widely used in maize genetics and breeding, because they are co-dominant, easy to score, highly abundant. In this study, we whole-genome sequences from 16 inbreds 1 wild relative determine SSR abundance develop a set of high-density polymorphic markers. A total 264 658 SSRs were identified across the 17 genomes, with an average 135 693 per genome. Marker density was one every 15.48 kb. (C/G)n, (AT)n, (CAG/CTG)n, (AAAT/ATTT)n most frequent motifs for...
The tricarboxylic acid (TCA) cycle is crucial for cellular energy metabolism and carbon skeleton supply. However, the detailed functions of maize TCA genes remain unclear.In this study, 91 were identified in by a homology search, they distributed on 10 chromosomes 1 contig. Phylogenetic results showed that almost all could be classified into eight major clades according to their enzyme families. Sequence alignment revealed several same subunit shared high protein sequence similarity....
Gray leaf spot (GLS), caused by the fungal pathogen Cercospora zeina (C. zeina), is one of most destructive soil-borne diseases in maize (Zea mays L.), and severely reduces production Southwest China. However, mechanism resistance to GLS not clear few resistant alleles have been identified. Two inbred lines, which were shown be (R6) susceptible (S8) GLS, injected C. spore suspensions. Transcriptome analysis was carried out with tissue at 0, 6, 24, 144, 240 h after inoculation. Compared 0...
Although C-type cytoplasmic male sterility (CMS-C) is one of the most attractive tools for maize hybrid seed production, detailed regulation network remains unclear. In order to identify CMS-C associated genes and/or pathways, comparison transcriptomes between line C48-2 and its isonuclear-alloplasmic maintainer N48-2 at pollen mother cell stage (PS), an early development microspore, mononuclear (MS), abortive were analyzed. 2,069 differentially expressed (DEGs) two stages detected thought...
C-type cytoplasmic male sterility (CMS-C) is widely utilized for hybrid maize seed production. However, genetic mechanisms underlying the fertility restoration are very complicated. At present, there a divergence on number of restorer genes in inbred line A619 CMS-C. To further elucidate restoring mechanism A619, we used analysis and molecular markers to confirm sterile C48-2 this study. Firstly, segregations (C48-2 × A619)F 2 populations were investigated under three environments during...
Callose contributes to many biological processes of higher plants including pollen development, cell plate and vascular tissue formation, as well regulating the transport function plasmodesmata. The functions callose synthase genes in maize have been little studied. We describe a male-sterile mutant 39 (ms39) characterized by reduced plant height. In this study, we confirmed using CRISPR/Cas9 technology that mutation Zm00001d043909 (ZmCals12), encoding synthase, is responsible for male...
Maize plant architecture influences planting density and, in turn, grain yield. Most of the architecture-related traits can be described as organ size. We describe a miniature maize mutant, Tiny 4 (Tip4), which exhibits reduced size multiple organs and semi-dominant monofactorial inheritance characteristic. Positional cloning confirmed that 4-bp deletion NAC TF with transmembrane motif 1-Like (NTL) gene ZmNTL2, denoted ZmNTL2Δ, confers Tip4 mutation. qRT-PCR showed ZmNTL2 was expressed all...
The transcription factor ZmbHLH16, the maize ortholog of OsTIP2 (OsbHLH142), was isolated in present experiment. Tissue expression analysis showed that ZmbHLH16 is preferentially expressed male reproductive organs. Subcellular location via rice protoplast indicated it located nucleus. Through nucleotide variation analysis, 36 polymorphic sites including 23 SNPs and 13 InDels, were detected among 78 inbred lines. Neutrality tests linkage disequilibrium experienced no significant evolutionary...
Abstract C-type cytoplasmic male sterility (CMS-C), one of the three major CMS types in maize, has a promising application prospect hybrid seed production. However, complex genetic mechanism underlying fertility restoration CMS-C remains poorly understood. The maize inbred line A619 is rare strong restorer lines carrying gene Rf4, but different segregation ratios are found several F2 populations derived from crosses between isocytoplasmic allonucleus and A619. In present study, fertile to...
A nuclear-encoded sigma (σ) factor is essential for the transcriptional regulation of plant chloroplast-encoded genes. Five putative maize σ factors have been identified by database searches, but their functions are unknown. We report a leaf color mutant etiolated/albino 1 (eal1) that was derived from space mutation breeding. The eal1 displays etiolated or albino leaves then gradually turn to normal green at seedling stage. changes in associated with photosynthetic pigment content and...