A5065208116- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Congenital heart defects research
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Protein Degradation and Inhibitors
- Genetics and Neurodevelopmental Disorders
- Bone health and treatments
- Bone Metabolism and Diseases
- Cardiac Fibrosis and Remodeling
- Histone Deacetylase Inhibitors Research
- Fault Detection and Control Systems
- Prenatal Screening and Diagnostics
- Alkaline Phosphatase Research Studies
- Graph Theory and Algorithms
- Receptor Mechanisms and Signaling
- Tissue Engineering and Regenerative Medicine
- Nuclear Structure and Function
- Signaling Pathways in Disease
- Cancer Genomics and Diagnostics
- Real-time simulation and control systems
- Pregnancy and preeclampsia studies
- Control Systems and Identification
Southern Medical University
2023-2024
Nanfang Hospital
2024
Peking University
2017-2020
Center for Life Sciences
2017-2019
Boston Children's Hospital
2017
Baylor College of Medicine
2017
Harvard Stem Cell Institute
2017
Harvard University
2017
Institute of Molecular Medicine
2017
Beijing Institute of Technology
2013
The eukaryotic genome is folded into higher-order conformation accompanied with constrained dynamics for coordinated functions. However, the molecular machinery underlying these hierarchically organized three-dimensional (3D) chromatin architecture and remains poorly understood. Here by combining imaging sequencing, we studied role of lamin B1 in dynamics. We found that depletion leads to detachment lamina-associated domains (LADs) from nuclear periphery global redistribution decompaction....
Rationale: Polycomb repressive complex 2 is a major epigenetic repressor that deposits methylation on histone H3 lysine 27 (H3K27me) and controls differentiation function of many cells, including cardiac myocytes. EZH1 EZH2 are alternative catalytic subunits with partial functional redundancy. The relative roles in heart development regeneration unknown. Objective: We compared the versus neonatal regeneration. Methods Results: Heart was normal Ezh1 −/− ( Ezh 1 knockout) Ezh2 f/f ::cTNT −Cre...
Rationale: We hypothesized that the differentiation processes of cardiac progenitor cell (CP) from first and second heart fields (FHF SHF) may undergo unique instructive gene regulatory networks or signaling pathways, precise SHF progression is contingent on FHF developmental cues. Objective: investigated how intraorgan communications control sequential building discrete anatomic regions at single-cell resolution. Methods Results: By transcriptomic analysis Nkx2-5 (NK2 homeobox 5) Isl1 (ISL...
ABSTRACT Exploration and dissection of potential actions effects long noncoding RNA (lncRNA) in animals remain challenging. Here, using multiple knockout mouse models single cell sequencing, we demonstrate that the divergent lncRNA Hand2os1/Uph has a key complex modulatory effect on expression its neighboring gene HAND2 subsequently heart development function. Short deletion Hand2os1 promoter diminishes transcription to ∼8-32%, but fails affect yields no discernable phenotypes....
In proliferating cells, where most Polycomb repressive complex 2 (PRC2) studies have been performed, gene repression is associated with PRC2 trimethylation of H3K27 (H3K27me3). However, it uncertain whether writing H3K27me3 mechanistically required for silencing. Here, we studied function in postnatal mouse cardiomyocytes, the paucity cell division obviates bulk rewriting after each cycle. EED (embryonic ectoderm development) inactivation heart (EedCKO) caused lethal dilated cardiomyopathy....
Cardiac macrophages/monocytes participate in maintaining homeostasis and orchestrating cardiac responses upon injury. However, the function of specific macrophage/monocyte subtypes related cell fate commitment mechanisms remain elusive regenerative nonregenerative hearts due to their cellular heterogeneities. Using spatiotemporal single-cell epigenomic analysis (P1) (P10) mouse post injury, we found that P1 accumulate reparative Arg1+ macrophages, while proinflammatory S100a9+Ly6c+ monocytes...
Rationale: Replication-independent histone turnover has been linked to cis-regulatory chromatin domains in cultured cell lines, but its molecular underpinnings and functional relevance adult mammalian tissues remain yet be defined. Objective: We investigated regulatory functions of replication-independent states postmitotic cardiomyocytes from mouse heart. Methods Results: used H2B-GFP (histone 2B-green fluorescent protein) fusion protein pulse-and-chase approaches measure rate...
Cell type-specific expression of the developmental gene is conferred by distinct enhancer elements. Current knowledge about mechanisms in Nkx2-5 transcriptional regulation and its specific roles multistage heart morphogenesis limited. We comprehensively interrogate enhancers U1 U2 controlling transcription during development. Serial genomic deletions mice reveal function redundantly to confer at early stages, but instead supports later stages. Combined markedly reduce dosage as E7.5, despite...
The control of the speed diesel engine is key to guarantee AC output quality DGS (Diesel Generator Set).Because heavy noise and pollution physical DGS, it a good way build mathematical model for research on speed.But theoretical models often were not accurate their algorithms had poor usability due nonlinearity soft parameters engine.To solve these problems, Hardware-in-the-Loop (HIL) simulation system has been built in this paper.In HIL system, power unit (includes engine, generator load...
ABSTRACT An efficient, generalizable method for genome-wide mapping of single-cell histone modifications or chromatin-binding proteins is so far lacking. Here we develop CoBATCH, co mbinatorial b arcoding a nd t argeted ch romatin release, profiling genomic distribution in cell culture and tissue. Protein A fusion to Tn5 transposase enriched through specific antibodies regions generates indexed chromatin fragments ready the library preparation sequencing. Importantly, combinatorial barcoding...
Osteoclast is critical in skeletal development and fracture healing, yet the impact underlying mechanisms of their metabolic state on these processes remain unclear. Here, by using osteoclast-specific small GTPase Rheb1-knockout mice, we reveal that mitochondrial respiration, rather than glycolysis, essential for cathepsin K (CTSK) production osteoclasts regulated Rheb1 a mechanistic target rapamycin complex 1 (mTORC1)-independent manner. Mechanistically, find coordinates with acetyl-CoA...
Abstract Single-cell measurement of chromatin states, including histone modifications and non-histone protein binding, remains challenging. We present a low-cost, efficient ChIP-seq (simultaneous indexing tagmentation-based ChIP-seq, itChIP), compatible to both low-input single cells for profiling states. This single-cell itChIP approach combines opening, simultaneous cellular tagmentation in tube, processing samples with tens rarity or thousands per assay, the entire procedure can be...
Large-scale network models based on transformer architecture have strong versatility in many fields. Due to the computation-intensive and large-scale characteristics of model, training domestic heterogeneous accelerators is restricted by aspects such as computing communication efficiency, resulting poor performance. Aiming at this problem, hot functions performance bottlenecks process are studied analyzed, corresponding optimization methods proposed hardware accelerators. In order solve...
Abstract Rigorous exploration and dissection of potential actions effects long noncoding RNA (lncRNA) in animals remain challenging. Here using multiple knockout mouse models single- cell sequencing, we demonstrate that the divergent lncRNA Hand2as has a key, complex modulatory effect on expression its neighboring gene HAND2 subsequently heart development function, largely independent transcription transcripts. Full-length deletion causes moderate yet prevalent upregulation hundreds cardiac...