A5025041192- Forensic Entomology and Diptera Studies
- Insect and Pesticide Research
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Advanced Fluorescence Microscopy Techniques
- Insect Utilization and Effects
- Insect Pest Control Strategies
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Streptococcal Infections and Treatments
- Cellular Mechanics and Interactions
- Animal Ecology and Behavior Studies
- Nuclear Structure and Function
- Hippo pathway signaling and YAP/TAZ
- Neurobiology and Insect Physiology Research
- Insect behavior and control techniques
- Heat shock proteins research
- Yersinia bacterium, plague, ectoparasites research
- Bacterial Genetics and Biotechnology
- Species Distribution and Climate Change
- Bacteriophages and microbial interactions
- Elasticity and Material Modeling
- Oral microbiology and periodontitis research
Soochow University
2023-2025
Capital Medical University
2021-2023
Beijing Anzhen Hospital
2021-2023
Peking University
2015-2021
Beijing Shijitan Hospital
2021
Pioneer (United States)
2019
Visualization of chromosomal dynamics is important for understanding many fundamental intra-nuclear processes. Efficient and reliable live-cell multicolor labeling loci can realize this goal. However, the current methods are constrained mainly by insufficient throughput, efficiency, flexibility as well photostability. Here we have developed a new approach to dual-color imaging based on modified single-guide RNA (sgRNA) CRISPR/Cas9 system. The modification sgRNA was optimized structure-guided...
Gene expression in response to stimuli underlies many fundamental processes. However, how transcription is regulated under these scenarios largely unknown. Here, we find a previously unknown role of nuclear actin transcriptional regulation. The RNA-seq data reveal that required for the serum-induced program. Using super-resolution imaging, found remarkable enhancement RNA polymerase II (Pol II) clustering upon serum stimulation, and this requires actin. Pol clusters colocalized with...
The eukaryotic genome is folded into higher-order conformation accompanied with constrained dynamics for coordinated functions. However, the molecular machinery underlying these hierarchically organized three-dimensional (3D) chromatin architecture and remains poorly understood. Here by combining imaging sequencing, we studied role of lamin B1 in dynamics. We found that depletion leads to detachment lamina-associated domains (LADs) from nuclear periphery global redistribution decompaction....
The CRISPR/Cas9 system has made significant contributions to genome editing, gene regulation and chromatin studies in recent years. High-throughput systematic investigations into the multiplexed biological systems require simultaneous expression coordinated functioning of multiple sgRNAs. However, current cotransfection based sgRNA coexpression remain inefficient, virus-based transfection approaches are relatively costly labor intensive. Here we established a vector-independent method...
Abstract Protein-protein interactions (PPIs) are critical for cellular activity regulation. Visualization of PPIs using bimolecular fluorescence complementation (BiFC) techniques helps to understand how implement their functions. However, current BiFC is based on fluorescent proteins and the brightness photostability suboptimal single molecule tracking experiments, resulting in either low spatiotemporal resolution or incapability extended time course. Here, we developed TagBiFC technique...
Liquid-liquid phase separation (LLPS) is an emerging phenomenon involved in various biological processes. The formation of phase-separated condensates crucial for many intrinsically disordered proteins to fulfill their functions. Using the recombinant protein reconstitute vitro has become standard method investigate behavior and function LLPS. Meanwhile, there urgent need characterize LLPS living cells. Importantly, formed through at physical relevant concentrations are often smaller than...
Bacterial binary cell division requires accurate placement of machinery. FtsZ, a vital component the machinery, can assemble into filaments and self-organize ring structure (Z ring) at appropriate site for division. MapZ, recently identified FtsZ regulator in Streptococcaceae, has been found to localize midcell where it helps properly position ring. However, its mechanism is still unclear. Here, by using total internal reflection fluorescence microscopy, super-resolution imaging, single...
A cost-effective and simple method for <italic>in situ</italic> labelling of genomic loci utilizing the DNA-guided DNA binding activity nuclease-deficient Argonaute protein in <italic>Thermus thermophilus</italic> (dTtAgo).
Summary Streptococcus mutans is a primary pathogen responsible for dental caries. It has an outstanding ability to form biofilm, which vital virulence. Previous studies have shown that knockout of Wall‐associated protein A (WapA) affects cell chain and biofilm formation S. . As surface protein, the distribution WapA remains unknown, but it important understand mechanism underlying function WapA. This study applied fluorescence mC herry as reporter gene characterize dynamic in via time‐lapse...
ABSTRACT The CRISPR/Cas9 system has made significant contribution to genome editing, gene regulation and chromatin studies in recent years. High-throughput systematic investigations into the multiplexed biological systems disease conditions require simultaneous expression coordinated functioning of multiple sgRNAs. However, current co-transfection based sgRNA co-expression remain poorly efficient virus-based transfection approaches are relatively costly labor intensive. Here we established a...
AT-hook DNA-binding motif-containing protein 1 (AHDC1) is a causal gene of intellectual disability/developmental delay in humans. The biological role AHDC1 unclear. Recently, some clues from mutation carriers hinted that may participate body-weight regulation. In this first metabolic phenotype study Ahdc1 deficiency, we generated Ahdc1-deficienct mouse line and found deficiency both male female mice led to adiposity weaning obesity characterized by reduced energy expenditure respiratory...
<title>Abstract</title> <italic>Dermestes frischii</italic> Kugelann, 1792 is a storage pest worldwide, and important for estimating the postmortem interval in forensic entomology. However, because of lack transcriptome genome resources, population genetics biological control studies on <italic>D. have been hindered. Here, single-molecule real-time sequencing next-generation were combined to generate full-length five developmental stages frischii</italic>, namely egg, young larva, mature...
Accurate postmortem interval estimation is vital in the investigation of homicides, suicides, and accidental deaths. It key narrowing suspect lists, improving crime-solving efficiency, offering solace to bereaved families. The intra-puparial period, comprising about half a fly's developmental cycle, presents challenges for morphological age estimation. External changes are limited color shifts appearance respiratory horns on puparium only within several hours after pupariation, while...