A5007802086- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Reproductive Biology and Fertility
- Tissue Engineering and Regenerative Medicine
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- Neurobiology and Insect Physiology Research
- Cancer-related molecular mechanisms research
- Neuroscience and Neuropharmacology Research
- Congenital heart defects research
- RNA Interference and Gene Delivery
- Insect Utilization and Effects
- Neural dynamics and brain function
- Circular RNAs in diseases
- Liver physiology and pathology
- Renal and related cancers
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- BRCA gene mutations in cancer
- COVID-19 Clinical Research Studies
Mayo Clinic in Arizona
2025
Tsinghua University
2015-2024
WinnMed
2024
Mayo Clinic
2019-2024
Shanxi Medical University
2024
Shenyang Normal University
2004-2021
Center for Life Sciences
2016-2021
Liaoning Normal University
2007-2021
Tokyo Medical and Dental University
2001-2021
Hainan Medical University
2017-2020
The pandemic of COVID-19, caused by SARS-CoV-2, is a major global health threat. Epidemiological studies suggest that bats (Rhinolophus affinis) are the natural zoonotic reservoir for SARS-CoV-2. However, host range SARS-CoV-2 and intermediate hosts facilitate its transmission to humans remain unknown. interaction coronavirus with receptor key genetic determinant cross-species transmission. uses angiotensin-converting enzyme 2 (ACE2) as enter cells in species-dependent manner. In this study,...
Histone modifications regulate gene expression and development. To address how they are reprogrammed in human early development, we investigated key histone marks oocytes embryos. Unlike that mouse oocytes, the permissive mark trimethylated H3 lysine 4 (H3K4me3) largely exhibits canonical patterns at promoters oocytes. After fertilization, prezygotic genome activation (pre-ZGA) embryos acquire chromatin widespread H3K4me3 CpG-rich regulatory regions. By contrast, repressive H3K27me3...
Organization of the genome into euchromatin and heterochromatin appears to be evolutionarily conserved relatively stable during lineage differentiation. In an effort unravel basic principle underlying folding, here we focus on itself report a fundamental role for L1 (LINE1 or LINE-1) B1/Alu retrotransposons, most abundant subclasses repetitive sequences, in chromatin compartmentalization. We find that homotypic clustering demarcates grossly exclusive domains, characterizes predicts Hi-C...
A major limitation in developing applications for the use of human embryonic stem cells (HESCs) is our lack knowledge their responses to specific cues that control self-renewal, differentiation, and lineage selection. HESCs are most commonly maintained on inactivated mouse fibroblast feeders medium supplemented with FCS, or proprietary replacements such as knockout serum-replacement together FGF-2. These undefined culture conditions hamper analysis mechanisms HESC behavior. We have now...
With the ability to manipulate cells temporarily and spatially into three-dimensional (3D) tissue-like construct, 3D bioprinting technology was used in many studies facilitate recreation of complex cell niche and/or better understand regulation stem proliferation differentiation by cellular microenvironment factors. Embryonic (ESCs) have capacity differentiate any specialized type animal body, generally via formation embryoid body (EB), which mimics early stages embryogenesis. In this study,...
Abstract Although the overall topography of cerebellar corticonuclear projection formed by Purkinje cell (PC) axons has been described, only a few studies have dealt with organization this at level individual PC axons. Thus, we reconstructed 65 single that were labeled biotinylated dextran amine in rat. We then analyzed relationship between projections these PCs and compartmentalization cortex nuclei based on olivocerebellar aldolase C expression PCs. After giving rise to short local...
3D printing has evolved into a versatile technology for fabricating tissue-engineered constructs with spatially controlled cells and biomaterial distribution to allow biomimicking of in vivo tissues. In this paper, we reported novel study cell lines derived from human embryonic kidney tissue macroporous tissue-like construct. Nozzle temperature, chamber temperature the composition matrix material were studied achieve high viability (>90%) after construct formation. Long-term stability clear...
The molecular mechanism controlling the zygotic genome activation (ZGA) in mammals remains poorly understood. 2-cell (2C)-like cells spontaneously emerging from cultures of mouse embryonic stem (ESCs) share some key transcriptional and epigenetic programs with 2C-stage embryos. By studying transition ESCs into 2C-like cells, we identified developmental pluripotency associated 2 4 (Dppa2/4) as important regulators program through directly up-regulating expression double homeobox (Dux). In...
Timing and trigger of cell polarization During mammalian embryo development, the first fate decision separates progenitors trophectoderm (destined to form placenta) from inner mass (which forms all tissues embryonic yolk sac). A key event for this lineage segregation is establishment apicobasal polarity. This set occur at a fixed developmental stage. The factors that polarity as well its temporal regulation have remained unknown. Zhu et al. show in mouse embryos three molecular...
Abstract The RNA-binding protein QKI belongs to the hnRNP K-homology domain family, a well-known regulator of pre-mRNA alternative splicing and is associated with several neurodevelopmental disorders. Qki found highly expressed in developing adult hearts. By employing human embryonic stem cell (hESC) cardiomyocyte differentiation system generating QKI-deficient hESCs (hESCs- del ) using CRISPR/Cas9 gene editing technology, we analyze physiological role differentiation, maturation,...
Abstract Dysfunctional immune responses contribute critically to the progression of Coronavirus Disease-2019 (COVID-19), with macrophages as one main cell types involved. It is urgent understand interactions among permissive cells, macrophages, and SARS-CoV-2 virus, thereby offering important insights into effective therapeutic strategies. Here, we establish a lung macrophage co-culture system derived from human pluripotent stem cells (hPSCs), modeling host-pathogen interaction in infection....
Background Several methods have been used to induce somatic cells re-enter the pluripotent state. Viral transduction of reprogramming genes yields higher efficiency but involves random insertions viral sequences into human genome. Although induced stem (iPS) can be obtained with removable PiggyBac transposon system or an episomal system, both approaches still use DNA constructs so that resulting cell lines need thoroughly analyzed confirm they are free harmful genetic modification. Thus a...
Abstract Sessile serrated adenomas/polyps ( SSA /Ps) are precursors of colon cancer, particularly those that exhibit microsatellite instability. Distinguishing /Ps from the related, but innocuous, microvesicular hyperplastic polyp MVHP ) can be challenging. In this study seven gastrointestinal pathologists reviewed 109 polyps and identified 60 with histological consensus. Microarray analysis was performed on six distal consensus MVHPs < 9 mm, proximal > normal biopsies (three proximal,...
Circular RNAs (circRNAs) represent a newly identified class of non‑coding RNA molecules, which interfere with gene transcription by adsorbing microRNAs (miRNAs). CircRNAs serve important roles in disease development and have the potential to as novel biomarkers for clinical diagnosis. However, role circRNAs occurrence gastric cancer (GC) remains unclear. In present study, expression profiles were compared between GC adjacent normal tissues using circRNA microarray, following quantitative...
The transcription factor BTB and CNC homology 1 (Bach1) is expressed in the embryos of mice, but whether Bach1 regulates self-renewal early differentiation human embryonic stem cells (hESCs) unknown. We report that deubiquitinase ubiquitin-specific processing protease 7 (Usp7) a direct target Bach1, interacts with Nanog, Sox2, Oct4, facilitates their deubiquitination stabilization via recruitment Usp7, thereby maintaining cell identity self-renewal. also polycomb repressive complex 2 (PRC2)...