A5101433646- Genetic and Kidney Cyst Diseases
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Retinal and Macular Surgery
- Mitochondrial Function and Pathology
- Pancreatic function and diabetes
- Hedgehog Signaling Pathway Studies
- Ocular Surface and Contact Lens
- Liver Disease Diagnosis and Treatment
- melanin and skin pigmentation
- Renal and related cancers
- Environmental Changes in China
- Lysosomal Storage Disorders Research
- Proteoglycans and glycosaminoglycans research
- RNA regulation and disease
- Birth, Development, and Health
- Corneal Surgery and Treatments
- Bladder and Urothelial Cancer Treatments
- Atherosclerosis and Cardiovascular Diseases
- Liver physiology and pathology
- Genetic Syndromes and Imprinting
- Vascular Malformations Diagnosis and Treatment
- Impulse Buying and Technology Impacts
- Protist diversity and phylogeny
Shandong Normal University
2020-2025
Chinese People's Liberation Army
2024
Renmin Hospital of Wuhan University
2023-2024
Affiliated Hospital of Guizhou Medical University
2024
Guiyang Medical University
2024
Wuhan University
2023-2024
Zhejiang Chinese Medical University
2022
State Key Laboratory of Membrane Biology
2018-2020
Peking University
2016-2020
Shandong University
2017-2019
The endoplasmic reticulum (ER) is composed of the nuclear envelope, perinuclear sheets and a peripheral tubular network. ER mitochondria form tight contacts at specific subdomains, which coordinate functions two organelles are required for multiple cellular processes such as Ca2+ transfer apoptosis. However, it largely unknown how morphology ER-mitochondria signaling dynamically regulated under different physiological or pathological conditions DNA damage. Here we show that peripheral,...
Abstract Activation of hepatic stellate cells (HSCs) plays a critical role in liver fibrosis. However, the molecular basis for HSC activation remains poorly understood. Herein, we demonstrate that primary cilia are present on quiescent HSCs but exhibit significant loss upon which correlates with decreased levels ciliary protein intraflagellar transport 88 (IFT88). Ift88 -knockout mice more susceptible to chronic carbon tetrachloride-induced Mechanistic studies show X-linked inhibitor...
T cell immunoglobulin and mucin-containing molecule 3 (Tim-3), expressed in dysfunctional exhausted cells, has been widely acknowledged as a promising immune checkpoint target for tumor immunotherapy. Here, using strategy combining virtual functional screening, we identified compound named ML-T7 that targets the FG-CC′ cleft of Tim-3, highly conserved binding site phosphatidylserine (PtdSer) carcinoembryonic antigen–related adhesion 1 (CEACAM1). enhanced survival antitumor activity primary...
Autophagosome and lysosome fusion is an important macroautophagy/autophagy process for cargo degradation, SNARE proteins, including STX17, SNAP29, VAMP7 VAMP8, are key players in this process. However, the manner which precisely regulated poorly understood. Here, we show that VAMP7B, a domain-disrupted isoform of R-SNARE protein VAMP7, competes with domain functional VAMP7A to bind STX17 inhibits autophagosome-lysosome fusion. Moreover, DIPK2A, late endosome- lysosome-localized protein,...
Cardiac involvement, displayed as premature cardiovascular disease (CVD), is one of common clinical symptoms patients with systemic lupus erythematosus (SLE), contributing to mortality the disease. The precise underlying pathological mechanism(s) for cardiac involvement in remains poorly understood. Lipids and their metabolites are directly involved atherosclerosis development, oxidative stress, inflammation, which closely related development CVD. In study, shotgun lipidomics was exploited...
Abstract Urogenital congenital anomalies (UCAs) is defined as “any live-birth with a urinary or genital condition” and affects millions of men women worldwide. However, sex differences related environmental risk factors in UCAs burden on global scale have not been assessed. Using data from the Global Burden Diseases, Injuries, Risk Factors Study (GBD) 2019, we estimated prevalence, incidence, mortality disability-adjusted life years (DALYs) 1990 to 2019 by sex, region, socio-demographic...
Production of melanin pigments is a protective mechanism the skin against ultraviolet (UV)-induced damage and carcinogenesis. However, molecular basis for melanogenesis still poorly understood. Herein, we demonstrate critical interplay between primary cilium melanocortin 1 receptor (MC1R) signaling. Our data show that UV α-melanocyte-stimulating hormone (α-MSH) trigger formation in human melanocytes melanoma cells. Deficiency MC1R or presence its red hair color (RHC) variations significantly...
Abstract The primary cilium is increasingly recognized as a crucial player in the physiology of biliary epithelial cells (BECs). However, precise role cilia development age‐related fibrosis remains unclear. Herein, using cilium‐deficient mice, we demonstrate that disruption ciliary homeostasis BECs aged mice leads to significant bile duct proliferation, augmented fibrosis, and heightened indicators liver injury. Our RNA‐sequencing data revealed dysregulation genes associated with various...
<title>Abstract</title> Aims Female pelvic organ prolapse (POP) is a disease associated with aging and inflammation, though it not determined that inflammation are causative factors. The purpose of this study was to evaluate the causal effects inflammatory factors on female (POP). Methods Significant genetic variables were evaluated by assessing genome-wide association (GWAS) data for POP 5 age biomarkers (GrimAge, HorvathAge, HannumAge, PhenoAge, leukocyte telomere length). Initially,...
Dry eye disease is a prevalent condition affecting 5%-50% of the global population. Animal model investigations play crucial role in understanding its underlying mechanisms. Therefore, we developed mouse dry by surgically removing both extraorbital lacrimal glands (ELG) and intraorbital (ILG) to investigate ocular surface pathology context aqueous deficiency eye. Two weeks post operation, mice exhibited severe manifestations, including reduced tear secretion, corneal epithelial...
<title>Abstract</title> Autophagy is a fundamental process for maintaining cell homeostasis, and STX17-mediated autophagosome-lysosome fusion essential cargo degradation recycling. The translocation of STX17 to the autophagosomal membrane necessary fusion, yet mechanism governing this remains largely unknown. Here, we show that cytosolic, but not membrane-inserted, translocates onto autophagosomes during autophagy. Following starvation, acetylated at lysine 254 (K254) which accessible only...
Neuregulin 4 (Nrg4) is a brown fat-enriched endocrine factor that ameliorates lipid metabolism disorders. Autophagy critical for pancreatic β-cell to counteract lipotoxicity-induced apoptosis. This study aimed at exploring whether Nrg4 attenuates apoptosis by regulating autophagy. The mouse line MIN6 was cultured in palmitic acid (PA) with or without administration. Apoptosis rate, together anti-apoptotic and pro-apoptotic protein levels, investigated. Autophagic flux autophagy-related...
Corneal inflammation, especially severe corneal plays a significant role in the development of limbal stem cell dysfunction. Constructing appropriate animal models can help us focus on effects inflammation cells. A 2 mm rust remover was used to remove central epithelium Sprague Dawley (SD) rats create an injury. Then, stroma cornea sutured with nylon sutures induce persistent inflammation. In this way, model abrasion and suturing constructed, which induced The changes condition cells at 1,...
The orientation and shape of chondrocytes in compressed articular cartilage were studied quantitatively by polarized light microscopy (PLM) at 0.34 μm pixel-resolution. morphological changes the cell shapes, orientation, territorial matrix surrounding quantified.
Objective To observe the different changes of macular microstructure in patients with large idiopathic hole (IMH) treated vitrectomy combined internal limiting membrane (ILM) transplantation or not. Methods Forty eyes 40 consecutive giant IMH (≥500 μm) were included study. Twenty received ILM (ILM group) and others peel off removal group). During operation, a proper size was removed filled bottom hole. The age, duration disease ocular laterality two groups not statistically...
Background Giant idiopathic macular hole (IMH) severely affects visual acuity and increases operative difficulty during the surgery, so modifying or optimizing operation method is helpful for improving prognosis. Objective This study aimed to evaluate efficacy safty of vitrectomy combined with free internal limiting membrane transplantation large IMH. Methods A prospective serial cases-observational was carried out under informed consent each patient.Forty-two eyes consecutive...