A5002323402- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Hypothalamic control of reproductive hormones
- Peptidase Inhibition and Analysis
- Hormonal Regulation and Hypertension
- RNA Interference and Gene Delivery
- Neuroendocrine regulation and behavior
- Chemical Synthesis and Analysis
- Cancer, Stress, Anesthesia, and Immune Response
- Evolution and Genetic Dynamics
- Stress Responses and Cortisol
- Corneal Surgery and Treatments
- Mathematical and Theoretical Epidemiology and Ecology Models
- Lipid Membrane Structure and Behavior
- Diabetes Treatment and Management
- Biochemical effects in animals
- Heat shock proteins research
- Connexins and lens biology
- Regulation of Appetite and Obesity
- Bacteriophages and microbial interactions
- Mesenchymal stem cell research
- Eosinophilic Disorders and Syndromes
- Adipokines, Inflammation, and Metabolic Diseases
- Signaling Pathways in Disease
Jinan University
2014-2024
Key Laboratory of Guangdong Province
2021-2024
Wenzhou University
2023-2024
Second Affiliated Hospital of Nanjing Medical University
2023
Chongqing Medical University
2022
University of Jinan
2010-2015
Cell fate and function can be regulated reprogrammed by intrinsic genetic program, extrinsic factors niche microenvironment. Direct reprogramming has shown many advantages in the field of cellular reprogramming. Here we tried possibility to generate corneal endothelia (CE) -like cells from human adipose-derived stem (ADSCs) non-genetic direct recombinant cell-penetrating proteins Oct4/Klf4/Sox2 (PTD-OKS) small molecules (purmorphamine, RG108 other chemical reagents), as well biomimetic...
Small-molecule positive allosteric modulator 1 (SPAM1), which targets pituitary adenylate cyclase-activating polypeptide receptor (PAC1-R), has been found to have a neuroprotective effect, and the underlying mechanism was explored in this study. First, using D-galactose (D-gal)-induced aging mouse model, we confirmed that SPAM1 improves structure of hippocampal dentate gyrus restores number neurons. Compared with D-gal model mice, SPAM1-treated mice showed up-regulated expression Sirtuin 6...
// Rongjie Yu 1, 2 , Hongyu Liu Xinhe Peng Yue Cui Suqin Song Like Wang Huahua Zhang 3 An Hong and Tianhong Zhou 4 1 Institute of Biomedicine, School Life Science Technology, Jinan University, Guangzhou, Guangdong, China National Engineering Research Center Genetic Medicine, Department Medical Genetics, Guangdong Dongguan, Bioengineering, Correspondence to: Yu, email: rongjie_yu1123@163.com Keywords: vasoactive intestinal peptide receptor (VPAC1), cysteine (Cys), palmitoylation, nuclear...
Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase activating polypeptide (PACAP) belong to the same family exert a variety of biological functions. Both PACAP VIP have protective effects in several tissues. While is known be stronger retinoprotective peptide, has very potent anti-inflammatory effects. The need for non-invasive therapeutic approach emerged been shown when administered form eye drops as well. cell penetrating TAT composed 11 amino acids tagging at C-terminus...
To investigate the protective effects of a novel cyclopeptide C*HSDGIC* (CHC) from cyclization Pituitary adenylate cyclase-activating polypeptide (PACAP) (1-5) in cellular and rodent models retinal ganglion cell apoptosis.Double-labeling immunohistochemistry was used to detect expression Thy-1 PACAP receptor type 1 line RGC-5. The apoptosis RGC-5 cells induced by 0.02 J/cm(2) Ultraviolet B irradiation. MTT assay, flow cytometry, fluorescence microscopy were viability, level reactive oxygen...
The high expression of PACAP (pituitary adenylate cyclase-activating polypeptide)-preferring receptor PAC1 is associated with nerve injury and tumors. Our previous report (Yu R, et al. PLoS One 2012; 7: e51811) confirmed the dimerization found that M-PAC1 mutation in N-terminal first Cys/Ala lost ability to form dimers. In this study, Chinese hamster ovary (CHO-K1) cells overexpressing wild-type (PAC1-CHO) had significantly higher anti-apoptotic activities against serum withdrawal-induced...
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a structurally endogenous peptide with many biological roles. Maxadilan, 61-amino acid vasodilatory peptide, specifically activates the PACAP type I receptor (PAC1). Although PAC1 has been identified in embryonic stem cells, little known about its presence or effects human induced pluripotent (iPS) cells. In present study, we investigated expression of iPS cells by reverse transcriptase polymerase chain reaction (RT-PCR) and...
Long non-coding RNAs (lncRNAs) has been proven by many to play a crucial part in the process of sepsis. To obtain better understanding sepsis, molecular biomarkers associated with it, and its possible pathogenesis, we obtained data from RNA-sequencing analysis using serum three sepsis patients healthy controls (HCs). Using edgeR (one Bioconductor software package), identified 1118 differentially expressed mRNAs (DEmRNAs) 1394 long noncoding (DElncRNAs) between HCs. We biological functions...
Abstract Pituitary adenylate cyclase‐activating polypeptide ( PACAP ) is a structurally endogenous peptide with many biological roles. However, little known about its presence or effects in human adipose‐derived stem cells hADSC s). In this study, the expression of type I receptor PAC 1R) was first confirmed s. Maxadilan, specific agonist 1R, could increase proliferation as determined by Cell Counting Kit‐8 and cell cycle analysis promote migration shown wound‐healing assays. Maxadilan also...
PAC1 is PACAP (pituitary adenylate cyclase-activating polypeptide) preferring receptor belonging to class B G protein coupled (GPCR) mediating the most effects of PACAP. The important role homo/heteromerization in folding, maturation, trafficking, and cell surface expression has become increasingly evident. bimolecular fluorescence complementation (BiFC) bioluminescence resonance energy transfer (BRET) assay were used this research confirm dimerization for first time. structure-activity...
Class B G-protein coupled receptors (GPCR) PAC1-R is a neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP)-preferring receptor that mediates the effective neuroprotective activity. Based on our previous data showing doxycycline and minocycline work as positive allosteric modulator (PAM) of PAC1-R, we used computer molecular docking isothermal titration calorimetry assay to further determine bindings doxycycline/minocycline's derivatives including tetracycline/tigecycline...