A5100438218- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Multiple Myeloma Research and Treatments
- Myxozoan Parasites in Aquatic Species
- Reproductive System and Pregnancy
- Mycobacterium research and diagnosis
- Immune cells in cancer
- Epigenetics and DNA Methylation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
- Cytokine Signaling Pathways and Interactions
- Phagocytosis and Immune Regulation
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Biology and Fertility
- Plant Pathogens and Fungal Diseases
- RNA modifications and cancer
- COVID-19 Clinical Research Studies
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Metal complexes synthesis and properties
- Autophagy in Disease and Therapy
- Toxoplasma gondii Research Studies
Fujian Medical University
2023-2025
Second Affiliated Hospital of Fujian Medical University
2024
Jinan University
2019-2024
Taizhou University
2024
Charité - Universitätsmedizin Berlin
2017-2024
Max Delbrück Center
2010-2024
Stanford University
2024
Shandong University
2012-2023
Insilico Medicine (United States)
2023
First Affiliated Hospital of Guangzhou Medical University
2018-2022
The accumulation of tumour-associated macrophages (TAMs) in the hypoxic tumour microenvironment (TME) is associated with malignant progression cancer. However, mechanisms by which TME facilitates TAM infiltration are not fully understood. This study showed that high ZEB1 expression cervical cancer cell islets was positively correlated CD163
To explore the mechanisms through which hypoxic tumor microenvironment (TME) modulates transition of tumor‐associated macrophages (TAMs). The migration ability RAW264.7 was determined by transwell assay. Flow cytometric, western blot and immunofluorescence analyses CD206 further validated M2 polarization macrophages. Immunofluorescence, qRT‐PCR were performed to detect expression neuropilin‐1 (Nrp‐1) carbonic anhydrase IX (CAIX). An intermittent hypobaric hypoxia (IH) animal model...
Abstract Clinical response to immunotherapy is closely associated with the immunosuppressive tumour microenvironment (TME), and influenced by dynamic interaction between cells lymphatic endothelial (LECs). Here, we show that high levels of miR-142-5p positively correlate indoleamine 2,3-dioxygenase (IDO) expression in tumour-associated vessels advanced cervical squamous cell carcinoma (CSCC). The transferred CSCC-secreted exosomes into LECs exhaust CD8 + T via up-regulation IDO expression,...
Objective.To explore the influence of M2-polarized tumor-associated macrophages (TAMs) on high-risk human papillomavirus (hr-HPV)-related cervical carcinogenesis and metastasis.Methods.CD68+ CD163+ were examined immunohistochemically in a series 130 samples, including 26 cases normal tissues, 59 intraepithelial neoplasia (CIN), 45 squamous cell carcinoma (SCC), results statistically analyzed.The macrophage count was corrected for epithelial stromal compartments respectively.Clinical data...
Tumor metastasis remains a major obstacle for improving overall cancer survival in cervical ( CC ), which may be due to the existence of tumor microenvironment‐related stem cells CSC s) and epithelial–mesenchymal transition EMT ). The mechanism underlying these processes needs further elucidated. Here, we report that TGF ‐β1, one key microenvironmental stimuli, can enhance characteristics, facilitate , induce CK 17. Silencing 17 expression attenuated ‐like properties without affecting...
Abstract Circulating neutrophil extracellular traps (NETs) resistant to t‐PA have not been studied completely although NETs in thrombi may contribute tissue plasminogen activator (t‐PA) resistance. This research intended elucidate whether circulating are associated with resistance and the underlying mechanism. The levels of were detected neutrophils, ischemic brain acute stroke (AIS) patients, transient middle cerebral artery occlusion (tMCAO) models. NET formation blood, thrombi, mice...
Lymphatic remodelling in the hypoxic tumour microenvironment (TME) is critically involved metastasis of cervical squamous cell carcinoma (CSCC); however, its underlying mechanisms remain unclear. Here, we uncovered a novel lymphatic pattern TME, wherein vessels (LVs) are encapsulated by tumour-associated macrophages (TAMs) to form an interconnected network. We describe these aggregates as LVEM (LVs TAMs) considering their advantageous metastatic capacity and active involvement early lymph...
Radioresistance remains a significant obstacle in the therapy of cervical cancer, and mechanism it is still unclear. We aimed to investigate role specificity protein 1 (Sp1) radioresistance cancer.Sp1 was examined immunohistochemically on tissues from 36 human cancer patients. used RT-qPCR Western blot examine expression Sp1 irradiated cell lines SiHa HeLa. The cells assessed by colony-formation assay cycle analysis. Dual-luciferase reporter performed detect downstream Sp1.High positively...
The precise recognition of specific peptide-MHC (pMHC) complexes by T-cell receptors (TCRs) plays a key role in infectious disease, cancer and autoimmunity. A critical step many immunobiological studies is the identification T-cells expressing TCRs to given pMHC antigen. However, intrinsic instability empty class-I MHCs limits their soluble expression Escherichia coli (E. coli) makes it very difficult characterize even small fraction possible pMHC/TCR interactions. To overcome this...
To investigate the determinants affecting live birth outcomes in fresh embryo transfer among polycystic ovary syndrome (PCOS) patients using various machine learning (ML) algorithms and to construct predictive models, offering novel insights for enhancing rates this specific group. A sum of 1,062 cycles involving PCOS were analyzed, with 466 resulting births. The dataset was split randomly into training testing subsets at a 7:3 ratio. Least absolute shrinkage selection operator recursive...
Adoptive transfer of T cell receptor–engineered (TCR-engineered) cells is a promising approach in cancer therapy but needs improvement for more effective treatment solid tumors. While most clinical approaches have focused on CD8+ cells, the importance CD4+ mediating tumor regression has become apparent. Regarding shared (self) antigens, it unclear whether human repertoire been shaped by tolerance mechanisms and lacks highly functional TCRs suitable therapy. Here, against tumor-associated...
Objective: The industrial production and combustion of coal can produce silica nanoparticles (nano-SiO2). It enters the human body mainly through respiratory tract exerts a toxic effect. However, whether nano-SiO2 increase IL-1β-induced inflammatory expression in A549 cells has not been tested. Therefore, synergistic toxicity IL-1β to was observed our study. Materials methods: We exposed (0, 100, 500, 1000 μg/ml) for 12 24 h. effect on viability by CCK-8 method. were (1 mg/mL) cytokine (10...
Abstract Background Systemic lupus erythematosus (SLE) is a complex systemic autoimmune disease characterized by the presence of numerous autoantibodies. The interaction infectious agents (viruses, bacteria and parasites) genetically susceptible host may be key mechanism for SLE. Toxoplasma gondii widespread intracellular parasite that has been implicated in pathogenesis diseases. However, relationship between T. infection increased risk SLE Chinese populations remains unclear. Methods...
Ferroptosis is a novel form of programmed cell death and considered to be druggable target for colorectal cancer (CRC) therapy. However, the role ferroptosis in CRC its underlying mechanism are not fully understood. In present study we found that protein enriched Golgi apparatus, phosphoprotein 3 (GOLPH3), was overexpressed human tissue several lines. The expression GOLPH3 significantly correlated with ferroptosis-related genes CRC. overexpression Erastin-induced Caco-2 cells reduced...
For thymic selection and responses to pathogens, T cells interact through their αβ cell receptor (TCR) with peptide–major histocompatibility complex (MHC) molecules on antigen-presenting cells. How the diverse TCRs a multitude of MHC is unresolved. It also unclear how humans generate larger TCR repertoires than mice do. We compared repertoire CD4 selected from single mouse or human class II (MHC II) in containing gene loci. Human yielded greater output more repertoire. The complementarity...
The aim of this study was to investigate the therapeutic effects and mechanism Wharton jelly-derived mesenchymal stem cells (WJ-MSC) in a spontaneous-abortion rat model.An abortion model established using intravenous injection bromocriptine. WJ-MSC were isolated cultured from jelly human umbilical cord. pathologic changes placenta decidual tissues observed after hematoxylin-eosin staining. embryo absorption rate counted. protein mRNA levels interleukin (IL)-10, interferon (IFN)-γ IL-17 each...
Bronchopulmonary dysplasia (BPD) is the most common type of chronic lung disease in infancy; however, there no effective treatment for it. In present study, a neonatal mouse BPD model was established by continuous exposure to high oxygen (HO) levels. Mice were divided randomly into 5 groups: control, BPD, EPO, MSCs, and MSCs+EPO. At 2 weeks post-treatment, vessel density expression levels endothelial growth factor (VEGF), stromal cell-derived factor-1 (SDF-1), its receptor C-X-C chemokine 4...
Multiple myeloma is a hematologic malignancy of monoclonal plasma cells that accumulate in the bone marrow. Despite their clinical and pathophysiologic relevance, roles marrow-infiltrating T treatment-naïve patients are incompletely understood. We investigated whether clonally expanded (i) were detectable multiple marrow, (ii) showed characteristic immune phenotypes, (iii) dominant clones recognized antigens selectively presented on cells. Single-cell index sorting T-cell receptor (TCR) αβ...
Generation of high avidity T cell receptors (TCRs) reactive to tumor-associated antigens (TAA) is impaired by tolerance mechanisms, which an obstacle effective therapies for cancer treatment. NY-ESO-1, a human cancer-testis antigen, represents attractive target such due its broad expression in different types and the restricted normal tissues. Utilizing transgenic mice with diverse TCR repertoire, we isolated TCRs against NY-ESO-1 157-165 HLA-A*02:01. We compared functions murine-derived...